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Desmoplakin I affinity mutant (F40-based tension sensor)

Plasmid

#119190

Purpose

The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.

Depositor

Alex Dunn

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)

Use

Transposon

Tags

mTFP1-F40-mEYFP

Expression

Mammalian

Mutation

inserted F40-based tension sensor module after aa…

Promoter

TCE

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pDONOR 6colors

Plasmid

#229974

Purpose

bigMamAct assembled plasmid for 6colors cellular labelling

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

mito-mCherry, EYFP-tubulin, H2B iRFP720, GTS-mTagBFP, mTFP1-actin

Use

CRISPR

Tags

Expression

Mammalian

Mutation

See Depositor Comments

Promoter

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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NK591

Plasmid

#109080

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mTFP1

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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dBrainbow

Plasmid

#29327

Depositor

Julie Simpson

Article

Hampel et al Nat Methods. 2011 Feb 6. ():.

Insert

UAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

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LV-Camk2-ChroMEd15-mTFP

Plasmid

#184240

Purpose

channelrhodopsin ChroME fused to mTFP

Depositor

Yiyang Gong

Article

Beck et al Biophys J. 2022 Mar 21. pii: S0006-3495(

Insert

ChroMEd15-mTFP

Use

Lentiviral

Tags

mTFP1

Expression

Mammalian

Mutation

deleted ChroME amino acids 317-331

Promoter

Camk2

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWC

Plasmid

#112903

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pNH043

Plasmid

#42155

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr_2pabPAC

Plasmid

#158971

Purpose

2P-activatable bPAC for lentivirus

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pNH058

Plasmid

#42161

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

TAP Tag::mTFP1

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pTORKm43GWC

Plasmid

#112898

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to geneticin (G418). Contains additional cassette for constitutive expression of mTFP1.

Depositor

Sebastian Schornack

Article

Evangelisti et al MBio. 2019 Oct 1;10(5). pii: mBio.01516-

Type

Empty backbone

Use

Oomycete expression

Tags

Expression

Mutation

Promoter

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pNH082

Plasmid

#42165

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2pabPAC

Plasmid

#158968

Purpose

2P-activatable bPAC for transient expression

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pNH013

Plasmid

#42147

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV_2pabPAC

Plasmid

#158969

Purpose

2P-activatable bPAC for AAV

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pNH078

Plasmid

#42164

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pNH030

Plasmid

#42149

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::linker::2xNLS

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pNH026

Plasmid

#42148

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::linker::TAP TAG

Use

Tags

TAP-Tag

Expression

Mutation

Promoter

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pNH066

Plasmid

#42162

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

N-TAP Tag-RT-::mTFP1

Use

Tags

TAP-TAg

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH094

Plasmid

#42167

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1-RT-::[(G4S)3]::C-TAP Tag

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pNH090

Plasmid

#42166

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::[(G4S)3]::C-TAP Tag

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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