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Showing: 1381 - 1400 of 13298 results

  7. pBS SK mCherryROSAegfp

    Plasmid
    #54322
    Purpose
    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.
    Depositor
    Silvestro Conticello
    Article
    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.
    Insert
    mCherry and EGFP
    Use
    CRISPR, Mouse Targeting, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    54322-plasmid-map-sequence-id-84459

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  8. pCas3

    Plasmid
    #229538
    Purpose
    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.
    Depositor
    Kevin Verstrepen
    Article
    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:
    Insert
    Cas3
    Use
    CRISPR
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    codon optimized for S.cerevisiae
    Promoter
    Available since
    Jan. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    229538-plasmid-map-sequence-id-459007

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  9. EB3-mNeonGreen

    Plasmid
    #98881
    Purpose
    In vivo visualization of microtuble plus ends (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    EB3
    Use
    Tags
    mNeonGreen
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    98881-plasmid-map-sequence-id-190942

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  10. 4xmt-mTurquoise2

    Plasmid
    #98819
    Purpose
    In vivo visualization of mitochondria (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    mts
    Use
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    98819-plasmid-map-sequence-id-214508

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  13. pQE30del

    Plasmid
    #106902
    Purpose
    pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106902-plasmid-map-sequence-id-327626

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  14. mScarlet-I-LaminB

    Plasmid
    #98831
    Purpose
    In vivo visualization of the nuclear envelope (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    CytERM
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    *(see below)
    Promoter
    CMV
    Available since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98831-plasmid-map-sequence-id-190908

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  19. pC0051-W85X REPAIR targeting guide cloned into pC0043

    Plasmid
    #103867
    Purpose
    Guide RNA expression plasmid for dPspCas13b that can be used to direct ADAR deaminase domain activity to the W85X mutation in the luciferase reporter plasmid pC0038
    Depositor
    Feng Zhang
    Article
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Insert
    W85X targeting guide RNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    103867-plasmid-map-sequence-id-199148

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  20. EB3-mScarlet-I

    Plasmid
    #98826
    Purpose
    In vivo visualization of microtuble plus ends (can be used for colocalization studies)
    Depositor
    Dorus Gadella
    Article
    Chertkova et al bioRxiv 160374
    Insert
    EB3
    Use
    Tags
    mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    98826-plasmid-map-sequence-id-190905

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Showing: 1381 - 1400 of 13298 results