We narrowed to 2,171 results for: Xpa

1. pKan-PCUP1-9myc-AID*(N)

   Plasmid

   #99527

   Purpose

   N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

   Depositor

   Helle Ulrich

   Article

   Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

   Insert

   IAA17(71-117) (AXR3 Mustard Weed)
   

   Use

   Pcr template for yeast tagging cassette

   Tags

   9xMyc

   Promoter

   CUP1

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC3111 (pSpy1C-Plac(3)_ffluc)

   Plasmid

   #218778

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Expression

   Bacterial

   Mutation

   deletion of mrfp cassette, mutagenesis of the RBS…

   Promoter

   Plac(3)

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJW1836

   Plasmid

   #154337

   Purpose

   Promoter reporter

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR
   

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJW1347

   Plasmid

   #154310

   Purpose

   Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa Linker for CT slot
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYSD313

   Plasmid

   #212931

   Purpose

   Encodes the Aga2 signal peptide, mRuby2 fluorescent protein HA-tagged Sed1p yeast surface display anchor protein with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   Sed1 (SED1 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT130_GalL_nCDA1-NGBE3

   Plasmid

   #145094

   Purpose

   Expressing base editor nCDA1-NGBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-NGBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAS_4xEcoLeuT(CUA)_EF1_sfGFP150TAG

   Plasmid

   #174892

   Purpose

   amber suppression reporter sfGFP150TAG with EcoLeuT(CUA) amber suppressor tRNA cassette

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG in sfGFP

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT2-7xTcf-NLS-CNL

   Plasmid

   #65712

   Purpose

   Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   7xTcf, minCMV, 3xNLS, CNL
   

   Use

   Luciferase

   Available Since

   Feb. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHJ4: LexA-VHH-Caffeine

   Plasmid

   #108242

   Purpose

   LexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.

   Depositor

   Jerome Bonnet

   Article

   Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

   Insert

   LexA DNA binding domain fusing with VHH-Caffeine antibody
   

   Use

   Synthetic Biology

   Promoter

   pLacO1

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-Gata2

    Plasmid

    #60665

    Purpose

    PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Gata2 (Gata2 Rat)
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-Tight-Puro_Olig1

    Plasmid

    #64841

    Purpose

    A lentiviral vector for the expression of mouse Olig1 under doxycyline control

    Depositor

    Paul Tesar

    Article

    Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

    Insert

    Olig1 (Olig1 Mouse)
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

    Plasmid

    #174891

    Purpose

    amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUDE1093

    Plasmid

    #204227

    Purpose

    Episomal yeast plasmid expressing the Ercas12a

    Depositor

    Jean-Marc Daran

    Article

    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

    Insert

    Eubacterium rectale cas12a (Ercas12a)
    

    Expression

    Yeast

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    Saccharomyces cerevisiae PGK1

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYPQ166-iSpyMac

    Plasmid

    #149553

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 genome editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    iSpyMacCas9
    

    Use

    CRISPR; Gateway compatible zispymaccas9 entry clo…

    Tags

    3X FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    R221K, N394K, Protospacer interacting domain form…

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA1 Flag ATXN1L

    Plasmid

    #33242

    Depositor

    Huda Zoghbi

    Article

    Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

    Insert

    ATXN1L (ATXN1L Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pTorPE-GEM-GECO1

    Plasmid

    #32463

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO1
    

    Use

    Lab constructed

    Tags

    6x His and modified TorA MNNNDLFQASRRRFLAQLG[G to…

    Expression

    Bacterial

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-Lmo2

    Plasmid

    #60666

    Purpose

    PiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoter

    Depositor

    James Thomson

    Article

    Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

    Insert

    Lmo2 (Lmo2 Mouse)
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TAC-OKMS-Cas9

    Plasmid

    #120353

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OKMS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR-SUMO1

    Plasmid

    #114388

    Purpose

    pDONR vector with SUMO1 gene

    Depositor

    Vincent Dion

    Article

    Aeschbach et al Sci Rep. 2017 Dec 21;7(1):18026. doi: 10

    Insert

    SUMO1 (SUMO1 Human)
    

    Expression

    Mammalian

    Mutation

    Q29H compared with NCBI reference NP_003343.1

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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