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We narrowed to 241 results for: Xpa

Showing: 1 - 20 of 241 results

  1. Genetic Code Expansion

    Type
    Collection
    ...Code Expansion Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian cells.... Yet in the past two decades, biologists have expanded the genetic code by redirecting specific codons...amino acids beyond the 20 standard amino acids. Expanding the Genetic Code In protein translation, an aminoacyl-tRNA...incorporated into the growing peptide chain. To expand the genetic code, modified tRNAs, codons, and tRNA... the existing protein translation machinery. To expand the genetic code, 4 major changes to the standard...stimuli, such as light. On a broader scale, the expanded genetic code can help us understand and evolve...

  3. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...  Second, expansion introduces distortions. The goal in expansion is for your sample to expand evenly in...equipment needed for EM or SRM? Enter Expansion Microscopy. What is Expansion Microscopy?  ExM provides a straightforward...the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules...allow for even expansion.  In some cases, labels are added after this stage.  Step 4: Expand! (Typically...are typically easier to expand than mouse brain slices, which are easier to expand than, say, a C. elegans...and after expansion and measure the degree of agreement between them to ensure the expanded images are...lines in pre-expanded samples can sometimes take on a fragmented appearance post-expansion, but that doesn...

  5. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...REC3, and PAM interacting domains and allows for expanded PAM recognition as well as increased specificity...synthetic gRNA) are derived from the same species. Expanding the CRISPR toolbox The isolation of novel CRISPR...

  6. Addgene is Expanding Our Viral Vector Service!

    Type
    Blog Post
    ...We’re very excited to announce that we are expanding our viral vector service to include a Packaged on... on Request option. Through this expansion, scientists will be able to request an AAV composed of an AAV... vector prep.    Excited? So are we! This expansion will give researchers increased flexibility when...when planning their research and expanded choice when it comes to ordering high-quality materials from...overview in this blog post.     When will the expansion happen?  You will be able to request Packaged ... their material page. Interested scientists can expand the banner to select their serotype of choice, ...resources.  We hope you’re as excited about this expansion as we are! Happy packaging! ...

  7. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical amino...introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and techniques...number of experiments with. The catch The rapid expansion of GCE led to the creation of many new tools and...Tan, Z., & Cornish, V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry...

  12. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... confluent, expand into larger vessels. A confluent well of a 6-well dish can be expanded into a 10 cm...reagent Day 3–14: Change media as needed Day 14–18: Expand and harvest stable cell lines Equipment Class II...undergo additional selection while the population expands. At this time, some researchers reduce the concentration...cm dish. A confluent 10 cm dish can be expanded into two 75 cm 2 flasks, etc. Pro-Tip This selection method...populations are growing well and have been sufficiently expanded, prepare cell stocks and/or harvest to test for...will have higher levels of expression. Consider expanding populations transduced with a variety of dilutions...Figure 1: Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9...

  13. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ... Monitor cells for growth and expand cells Day 14–30: Analyze and expand monoclonal lines of interest ...cells have expanded but before they become over-confluent, trypsinize the cells and expand them to larger...After the monoclonal lines have been sufficiently expanded, screen the lines for transgene expression and...Figure 1: Generation of monoclonal cell lines from expansion of individual A549 cells stably expressing Cas9... in individual wells of a 96-well plate after expansion of single cells for 13 days. Note that growth ...Single cells from a stable cell pool were then expanded for an additional 3 weeks under blasticidin selection...stable cell pool (pool) and from individual cell expansions (monoclonal, 1-5). Wild-type A549 cells do not...

  14. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...protein function and signaling networks continues to expand. The Rinehart and Söll labs at Yale University ...Noren, C. J., Rinehart, J., & Söll, D. (2011). Expanding the Genetic Code of Escherichia coli with Phosphoserine...Additional Resources See our dedicated Genetic Code Expansion collection page Read about Using Phosphoserine...Phosphorylation Read about GCE4All: Making Genetic Code Expansion Accessible...

  16. p53 Pathway

    Type
    Collection
    ...The list of p53 transcriptional targets is ever-expanding; the best-characterized effects of p53 are in ... have been characterized as gain-of-function, expanding the mechanisms by which mutant p53 is known to...Oct;9(10):701-13. PubMed PMID: 19693097 . The expanding universe of p53 targets. Menendez D, Inga A, Resnick...

  17. Video Library

    Type
    Protocol
    ... offers career advice for scientists looking to expand their international reach. Joel McDade Joel McDade...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...blue, green, red, and ratiometric indicators An Expanded Palette of Genetically Encoded Ca2+ Indicators...Genetically-encoded yellow fluorescent cAMP indicator with an expanded dynamic range for dual-color imaging. PLoS One...(cGull) Generation of a cGMP Indicator with an Expanded Dynamic Range by Optimization of Amino Acid Linkers...encoded dopamine sensor YdLight1 and RdLight1 An expanded palette of dopamine sensors for multiplex imaging...

  19. CRISPR Guide

    Type
    Collection
    ...effects xCas9 3.7 - mutations in multiple domains to expand PAM recognition, increase specificity, and decrease...recognizes NNTA, a PAM sequence often found in plants, expanding the targeting range for plant genome engineering...cleave ssDNA non-specifically. Cas12a also has an expanded targeting range to AT-rich regions, as it recognizes.... (2018). Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science , 361 (6408), 1259–1262...
Showing: 1 - 20 of 241 results