...  Second, expansion introduces distortions. The goal in expansion is for your sample to expand evenly in...equipment needed for EM or SRM? Enter Expansion Microscopy. What is Expansion Microscopy?  ExM provides a straightforward...the protocol, samples can be expanded to ~4-10x linearly! This expansion physically separates molecules...allow for even expansion.  In some cases, labels are added after this stage.  Step 4: Expand! (Typically...are typically easier to expand than mouse brain slices, which are easier to expand than, say, a C. elegans...and after expansion and measure the degree of agreement between them to ensure the expanded images are...lines in pre-expanded samples can sometimes take on a fragmented appearance post-expansion, but that doesn...

...may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical amino...introduced to the bacteria using plasmids. Rapid expansion occurred, with hundreds of new ncAAs and techniques...number of experiments with. The catch The rapid expansion of GCE led to the creation of many new tools and...Tan, Z., & Cornish, V. W. (2021). Genetic Code Expansion: A Brief History and Perspective. Biochemistry...

...REC3, and PAM interacting domains and allows for expanded PAM recognition as well as increased specificity...synthetic gRNA) are derived from the same species. Expanding the CRISPR toolbox The isolation of novel CRISPR...

...et al., 2016) and since then has been trying to expand their precision editing capabilities. Base editors...with many Cas domains beyond SpCas9. In order to expand the gene-editing applications and targeting scope...

...scientific sharing The EDGE Program is designed to expand the number of systems that can be studied by providing...

...connections. Expanding the viral vector knowledge base: You can help! We’ve been expanding our viral vector...ready-to-use viral preps and we’ll be continually expanding our collection. We’ll provide a brief rundown ...find our updated viral vector pages as well as expanded virus protocols, information on how we produce...email to [email protected]. Your questions help us expand and improve our resources for future users. We’...

...merely 20. Expanding RNA Editing Capabilities through RESCUE Two years later, the Zhang lab expanded the RNA...With the expanded targeting ability using both systems, the authors look forward to the expanded use of ...precise RNA editing. The addition of RESCUE further expands the RNA editing toolkit and sequences that can ...

...distribution was limited to academic scientists. Expanding plasmid sharing during COVID-19 Realizing this...to industry scientists  While we’ve focused on expanding COVID-19 plasmids to industry scientists in the...that support us in our mission.  While rapidly expanding our COVID-19 resources has been our focus for ...transfer office to discuss the possibilities for expanded sharing. Is there a plasmid you're hoping will...

... lab cooler boxes Some vendors take back their expanded polystyrene (a.k.a. Styrofoam or cooler boxes)...when receiving temperature-controlled reagents. Expanded polystyrene will likely end up in a landfill, ...associated with CO2 emissions. NEB takes back expanded polystyrene boxes for free (in the U.S.). Their... program in 1976! Millipore Sigma takes back expanded polystyrene boxes for free (in the U.S.). After...

...they meet other Mentors and Singletons, and vastly expand their networks as they naturally find people with...briannadoesbio) June 22, 2019 Favorable conditions - Expansion of networks The last stage is Exponential Growth...mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons to other scientific conferences...such mentoring programs, scientists can build and expand their networks and further their career development...

...Seeding Labs, a nonprofit founded in 2007, strives to expand the opportunities and scientific resources available...first step; going forward, Seeding Labs hopes to expand its activities by facilitating connections to mentorship...regions like South America and Africa, but we need to expand our efforts to find new and creative ways to reach...

...physiology, development, and disease. In order to expand their model’s impact, organoid users have recently...months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9... INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON...

...for light mediated neuronal control and we are expanding our viral service to make these readily available... experiment. The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes... is vital in studying neural networks. We have expanded our calcium sensor collection to include our first...

...hundreds of scientists about various ways we might expand Addgene’s services. There was an overwhelmingly... to facilitate the formation of partnerships to expand the service and figure out what we can afford to...educational content via the Addgene Blog and our expanding website resources. I hope this gave you the impression...

...Our previous blog post The PAM Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity...toolkit available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to ..., et al. "Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259...

...smartphone that can now run several more apps. The expanded functions of this “CRISPR 2.0” include gene regulation...multiple functions using a single dCas9 and an expandable library of PUF-effector modules. The components...will be available at Addgene soon. We will also expand the collection and a database of orthogonal Casilio...

...).This toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst...Podcast Segment    Photocleavable protein PhoCl expands optogenetics toolbox Increasingly popular Optogenetics...proteins (FP) for cellular imaging is constantly expanding. Case in point - check out the growing list of...new base editors with distinct PAM sequences, expanding the number of available target sites for base ...

...enzyme. Fast facts – PAM requirement: NGG and an expanding number of others. Best for: a wide range of DNA...scientist toolbox. If your genome engineering goals expand beyond generating frameshift mutations or single...my CRISPR experiment? The PAM requirement and expanding CRISPR beyond spCas9 Cas proteins More resources...