We narrowed to 4,170 results for: spl
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Plasmid#118655PurposeTransient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway DonorDepositorInsertSMN2-DN-RG6-SA
UseCRISPRExpressionMammalianPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1808_pmax-RBFOX1N-FRB-C
Plasmid#118641PurposeTransient transfection; Expresses RBFOX1N-FRB-C; CAGGS promoterDepositorInsertRBFOX1N-FRB-C
UseCRISPRExpressionMammalianPromoterCAGGSAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1816_pCR8-gSMN2-DN4
Plasmid#118649PurposeTransient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway DonorDepositorInsertgSMN2-DN4
UseCRISPRExpressionMammalianPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
BAP1-010
Plasmid#68366Purposestable expression of BAP1 delta 221-238 in mammalian cellsDepositorInsertBAP1 (BAP1 Human)
ExpressionMammalianMutationdelta 221-238, splice variant found in human meso…PromoterCMVAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
TagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-KRAS-G12V
Plasmid#179654PurposeKRAS active mutant (G12V mutation)DepositorInsertKRAS G12V
ExpressionMammalianMutationG12VAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBFv-UAS3-GFP1-10
Plasmid#138398PurposeExpresses split GFP fragment (GFP1-10)DepositorInsertGFP1-10
ExpressionInsectAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
V143 pLP-HA SD - Acceptor
Plasmid#11710DepositorTypeEmpty backboneUseCre/Lox; Acceptor vectorTagsHAExpressionMammalianAvailable sinceJune 22, 2006AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMS59
Plasmid#215680PurposeCas9 + guide plasmid targeting ChrI split hygromycinR landing padDepositorInserteef-1A.1p::Cas9 + U6p::GTTTGAGTAGAGCACTCAGG
UseCRISPRExpressionWormAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYSD085
Plasmid#212922PurposeEncodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)DepositorInsert649 Stalk
ExpressionBacterialAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
SPDK3182
Plasmid#97404Purposebinary construct of ER-targeted sfYFP1-10 for plant expressionDepositorInsertER-sfYFP1-10
TagsER targetExpressionPlantAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
SPDK3184
Plasmid#97405Purposebinary construct of cytosolic sfCFP1-10 tag for plant expressionDepositorInsertsfCFP1-10
TagscytosolExpressionPlantAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CMVTO-SEAP-26Sfur-B2AD-tevs-KKMP
Plasmid#179636PurposeTEVP-inducible single TM SEAP RELEASE constructDepositorInsertSEAP RELEASE
ExpressionMammalianAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD101
Plasmid#131402PurposeE. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD101
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBS956 IBB-GFP-mCherry3E
Plasmid#107859PurposeFlow cytometry-compatible reporter for TDP43 function in splicingDepositorInsertSynthetic splicing reporter (TARDBP Synthetic)
ExpressionMammalianAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PEP114E
Plasmid#97400Purposebinary construct of ER-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
TagsER targetExpressionPlantAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CTE-Luc
Plasmid#60881PurposeReporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTEDepositorInsertLuc-CTE
ExpressionMammalianAvailable sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMS-dsRed2-C1824U LMNA-GFP minigene
Plasmid#128231PurposeC1824U mutation Lamin A splicing reporterDepositorInsertlamin A (LMNA Human)
TagsGFP and SV40-driven dsRed2 to identify transfecte…ExpressionMammalianMutationC1824UPromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(WT)
Plasmid#137826PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(WT)
Tags3x FLAGExpressionMammalianPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNL(CMV)Fluc/CMV/WPREdU3
Plasmid#136881PurposeReporter vector expression firefly luciferaseDepositorInsertFirefly luciferase
UseLentiviralAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only