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pET28-hisSumo-KE-Des27.11

Plasmid

#248006

Purpose

Expression of synthetic Kemp eliminase (Des27.11)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.11

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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mCerulean-T-SpyTag

Plasmid

#243787

Purpose

Expresses multifunctional construct enabling the YES-gated release of mCerulean from materials in response to TEV treatment. Construct contains a SpyTag for material tethering.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

mCerulean

Tags

6x His tag

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pPD0053_pIVTRup_EBFP2

Plasmid

#242544

Purpose

IVT

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

EBFP2

Use

Other

Available Since

Nov. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7

Plasmid

#248003

Purpose

Expression of synthetic Kemp eliminase (Des27.7)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.9

Plasmid

#248004

Purpose

Expression of synthetic Kemp eliminase (Des27.9)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.9

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.13

Plasmid

#248008

Purpose

Expression of synthetic Kemp eliminase (Des27.13)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.13

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pIVT-mRuby2-P2A-PuroR_pKG02755

Plasmid

#246371

Purpose

IVT template for making modRNA encoding mRuby2-P2A-PuroR

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

mRuby2-P2A-PuroR

Use

Synthetic Biology; In vitro transcription

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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Multi-Janus-Spacer-1x_pKG1126

Plasmid

#246375

Purpose

Multi-Janus spacer for Galloway Lab Golden Gate cloning scheme. Used as a "filler" plasmid in transfection experiments.

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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TET-ZF37-mCherry-BGH_pKG01502

Plasmid

#246377

Purpose

ZFa Plasmid expressing VP16-ZF37-2A-mCherry from the TET promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF37-2A-mCherry

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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TET-ZF43-TagBFP-BGH_pKG01531

Plasmid

#246378

Purpose

ZFa Plasmid expressing VP16-ZF43-2A-TagBFP from the TET promoter

Depositor

Kate Galloway

Article

Kabaria et al Nat Biotechnol. 2025 Oct 13. doi: 10.103

Insert

VP16-ZF43-2A-TagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

none

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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