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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Article

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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SunTag-FWAgRNA-4-22aa-TET1cd

Plasmid

#106435

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-SOUL-P2A-TdTomato-WPRE-hGH-polyA

Plasmid

#177576

Purpose

Expresses SOUL in neuronal cell types

Depositor

Guoping Feng

Article

Gong et al Neuron. 2020 Jul 8;107(1):197. doi: 10.1

Insert

SOUL

Use

AAV

Tags

tdTomato

Expression

Mutation

Promoter

hSyn

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_NLS-SaCas9-NLS-VPR

Plasmid

#68496

Purpose

AAV vector containing SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SaCas9-VPR

Use

AAV and CRISPR

Tags

VPR

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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SunTagng14aa

Plasmid

#106439

Purpose

Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.2 (20-362)

Plasmid

#177845

Purpose

Bacterial Expression of SnRK2.2

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2 (SNRK2.2 Mustard Weed)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-p21_T145A/S146A

Plasmid

#42623

Depositor

Philipp Mergenthaler

Article

Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

Insert

p21 (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

T145A/S146A

Promoter

CAG

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pMDC32B-AtmiR173aTS-B/c

Plasmid

#227965

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
AtmiR173aTS

Use

Tags

Expression

Plant

Mutation

Promoter

35S and None

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-DHFR-3xFLAG

Plasmid

#217428

Purpose

Lentiviral overexpression of human DHFR

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert sequence is a codon-optimized gBLOCK (IDT)

Promoter

CMV

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgABCC4_1

Plasmid

#217433

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 1 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgABCC4_3

Plasmid

#217434

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 3 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AtSLAC1 WT

Plasmid

#212946

Purpose

Vector used for expression of AtSLAC1 WT in mammalian cell

Depositor

Sangho Lee

Article

Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

Insert

Slow anion channel-associated 1 (OZS1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-AtSLAC1 6D

Plasmid

#212947

Purpose

Vector used for expression of AtSLAC1 6D in mammalian cell

Depositor

Sangho Lee

Article

Lee et al Nat Commun. 2023 Nov 14;14(1):7345. doi:

Insert

Slow anion channel-associated 1 (OZS1 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

T62D/S65D/S107D/S124D/S146D/S152D

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.3 (19-361)

Plasmid

#177846

Purpose

Bacterial Expression of SnRK2.3

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.3 (SNRK2.3 Mustard Weed)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-5'Luciferase(split CAGGT)_BDlacZ-SV40polyA

Plasmid

#216320

Purpose

Split luciferase assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Luciferase + splice donor site

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSUMO His6 SUMO SnRK2.6 (11-362)

Plasmid

#177847

Purpose

Bacterial Expression of SnRK2.6

Depositor

Karsten Melcher

Article

Ng et al Proc Natl Acad Sci U S A. 2011 Dec 27;10

Insert

SNF1-related protein kinase 2.6 (OST1 Mustard Weed)

Use

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVneo-DEST

Plasmid

#119746

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

Depositor

Andrew Jackson, Martin Reijns

Article

Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pK18-hACE2

Plasmid

#149449

Purpose

Expresses human ACE2 driven by the K18 promoter

Depositor

Paul McCray

Article

McCray PB et al J Virol. 2007 Jan;81(2):813-21. doi: 10.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

K18

Available since

June 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

Plasmid

#216319

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cerulean + splice acceptor site

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

Plasmid

#216318

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

split cerulean fluorescent protein + splice donor site

Use

AAV

Tags

Expression

Mutation

Promoter

CMV

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge