We narrowed to 5,102 results for: codon optimized

1. pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His

   Plasmid

   #218198

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOM

   Depositor

   Florence Pojer

   Insert

   EndoCOM
   

   Tags

   3C-CBD-His

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 19-315

   Promoter

   T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. m.3047-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #211339

   Purpose

   Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

   Depositor

   Monkol Lek

   Insert

   m.3047-Left TALE-G1397-N-dead-mCherry
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. m.3075-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #211340

   Purpose

   Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

   Depositor

   Monkol Lek

   Insert

   m.3075-Left TALE-G1397-N-dead-mCherry
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. m.5147-Left TALE-G1397-N-dead-mCherry

   Plasmid

   #211341

   Purpose

   Expression of codon-optimized dead mitochondrial base editor in human cells; cell sorting; blasticidin drug selection

   Depositor

   Monkol Lek

   Insert

   m.5147-Left TALE-G1397-N-dead-mCherry
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET3-ARL1-His6 (CO)

   Plasmid

   #201866

   Purpose

   Used for bacterial expression of human protein ARL1

   Depositor

   Richard Kahn

   Insert

   ARL1 (ARL1 Human)
   

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hPhyB621(Y276H)-P2A-m1V

   Plasmid

   #197377

   Purpose

   The AAV vector for PhyB621(Y276H) and m1V

   Depositor

   Michiyuki Matsuda

   Insert

   Phytochrome B Y276H(1-621 aa)
   

   Use

   AAV

   Tags

   P2A-m1V

   Mutation

   Human codon optimized PhyB (1-621 aa), Y276H muta…

   Promoter

   CMV

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET3-ARF3-His6(CO)

   Plasmid

   #201884

   Purpose

   Used for bacterial expression of human protein ARF3

   Depositor

   Richard Kahn

   Insert

   ARF3 (ARF3 Human)
   

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHH0103 TCERG1L(ORF-2) WW domain #1

   Plasmid

   #104294

   Purpose

   Bacterial expression of WW domain #1 from TCERG1L(ORF-2)

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   TCERG1L(ORF-2) WW domain #1 (TCERG1L Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHH0103 HYPB WW domain

   Plasmid

   #104267

   Purpose

   Bacterial expression of WW domain from HYPB

   Depositor

   Sachdev Sidhu, Marius Sudol

   Insert

   HYPB WW domain (SETD2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   codon optimized for expression in bacteria

   Promoter

   tac

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103 HYPC WW domain #2

    Plasmid

    #104232

    Purpose

    Bacterial expression of WW domain #2 from HYPC

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    HYPC WW domain #2 (PRPF40B Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid

    #107941

    Purpose

    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized enAsCas12a (E174R/S542R/K548R)
    

    Tags

    3x HA and NLS (nucleoplasmin)

    Expression

    Mammalian

    Mutation

    E174R, S542R and K548R

    Promoter

    CAG

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas12Max-ABE

    Plasmid

    #195335

    Purpose

    vector for encoding a human codon-optimized dCas12Max-ABE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized dCas12Max-ABE
    

    Expression

    Mammalian

    Mutation

    N243R, E336R, D1049A

    Promoter

    CBh, CMV, hU6

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCJ190

    Plasmid

    #162667

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTol2-UAS-zmiRFP

    Plasmid

    #108415

    Purpose

    Codon-optimized monomeric near-infrared fluorescent protein miRFP in zebrafish expression plasmid

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

    Insert

    zmiRFP
    

    Use

    Zebrafish

    Promoter

    UAS

    Available Since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. hfCas12Max

    Plasmid

    #195336

    Purpose

    vector for encoding a human codon-optimized hfCas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized hfCas12Max
    

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    N243R, E336R, D892R

    Promoter

    CBh, CMV, hU6

    Available Since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid

    #112203

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W159H, S238F

    Promoter

    N/A

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-21b(+)-Is-PETase-W185A

    Plasmid

    #112204

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W185A

    Promoter

    N/A

    Available Since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid

    #208336

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CAG

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCJ189

    Plasmid

    #162666

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. dCas12Max-CBE

    Plasmid

    #195337

    Purpose

    vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized dCas12Max-CBE
    

    Expression

    Mammalian

    Mutation

    N243R, E336R, D1049A

    Promoter

    CBh, CMV, hU6

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge