We narrowed to 10,315 results for: plasmids 101

1. SP_A1

   Plasmid

   #228145

   Purpose

   Plasmid contains the secretion signal peptide sequence of the yeast MEL1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   sp_P04824_MEL1_YEASX:0-18
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP_A2

   Plasmid

   #228146

   Purpose

   Plasmid contains the secretion signal peptide sequence of the yeast (S. uvarum) SPR1 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   sp_Q876J2_SPR1_SACU7:0-20
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. SP_A3

   Plasmid

   #228147

   Purpose

   Plasmid contains the secretion signal peptide sequence of the human ECM33 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   sp_P38248_ECM33_YEAST:0-19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SP_A4

   Plasmid

   #228148

   Purpose

   Plasmid contains the secretion signal peptide sequence of the human CHADL gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   sp_Q6NUI6_CHADL_HUMAN:0-30
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SP_A5

   Plasmid

   #228149

   Purpose

   Plasmid contains the secretion signal peptide sequence of the human GDF8 gene for use in Combinatorial Golden Gate Assembly (BsaI restriction sites).

   Depositor

   Joseph Brock

   Article

   Cleaver et al Trends Biotechnol. 2025 Apr;43(4):838-86

   Insert

   sp_O14793_GDF8_HUMAN:0-18
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-Syn1-DIO-Flpo

   Plasmid

   #237222

   Purpose

   For Cre-dependent expression of Flp recombinase

   Depositor

   Thomas Hnasko

   Article

   Oriol et al bioRxiv [Preprint]. 2025 Jan 17:2024.06.

   Insert

   Flpo recombinase
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL4.11_Nued2_E1_dAP1-2

   Plasmid

   #203837

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 1 with both AP-1 sites knocked out

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dAP1-2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   KO of both AP1 sites

   Promoter

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL4.11_Nued2_E1_dAP1

   Plasmid

   #203835

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 1 containing only the downstream AP1 site

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dAP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   KO of AP1 upstream

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL4.11_Nued2_E1_dAP2

   Plasmid

   #203836

   Purpose

   Luciferase reporter plasmid for Igf1 enhancer 1 containing only the upstream AP1 site

   Depositor

   Ivo Spiegel

   Article

   Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

   Insert

   dAP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   KO of AP1 downstream

   Promoter

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pgTS40

    Plasmid

    #169630

    Purpose

    pX330 derived vector for PCAG driven expression of SpCas9 and PU6 driven expression of guide RNA OGTS40 (5' GGGGCCACTAGGGACAGGAT 3') targeting position 55115755 of chromosome 19.

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    U6-driven gRNA expression and PCAG-driven SpCas9 expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 / PCAG

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTS1041

    Plasmid

    #169619

    Purpose

    Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::A2-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pVH228-Tier1-OVanO2-PCMVmin2-SEAP

    Plasmid

    #169564

    Purpose

    Tier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    vanillic acid-controlled SEAP production
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    VanO2-PCMVmin

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTS1040

    Plasmid

    #169618

    Purpose

    Tier-2 vector encoding PPGK-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (A1-pA::PPGK-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven expression of secreted Nluc and mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PPGK

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTS1039

    Plasmid

    #169617

    Purpose

    Tier-2 vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 (PhCMV-SEAP-p2A-iRFP670-pA::A2-pA::A3-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven expression of SEAP and iRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP

    Plasmid

    #169588

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-2

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTS2366-Tier1-PhCMV_F-type

    Plasmid

    #169567

    Purpose

    Tier-1 vector encoding PhCMV-driven F-type transactivator domain (PhCMV-F-type-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven F-type VP16
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pVH255-Tier1-OTetO7-PCMVmin1-SEAP

    Plasmid

    #169587

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-1-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TetO7-PCMVmin-1

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVH321-1-Tier1-PhCMV-dCas9-3xNLS

    Plasmid

    #169595

    Purpose

    Tier-1 vector encoding PhCMV-driven dCas9-3xNLS expression (PhCMV-dCas9-3xNLS-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    dead S.pyogenes Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTS2385-Tier1-PhCMV-fastFT

    Plasmid

    #169518

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "fast" maturation fastFT (PhCMV-fastFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven fluorescent timer, fast maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTS2384-Tier1-PhCMV-medFT

    Plasmid

    #169517

    Purpose

    Tier-1 vector encoding PhCMV-driven delayed-maturing fluorescent protein fluorescent timer (FT) with "medium" maturation medFT (PhCMV-medFT-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PCMV-driven fluorescent timer, medium maturation time
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PhCMV

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge