We narrowed to 10,315 results for: plasmids 101
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Plasmid#169503PurposeTier-1 vector encoding PhCMV-controlled tabacco etch virus protease recognition site (PhCMV-TEVsite-pA).DepositorInsertPCMV-driven tobacco etch virus protease cleavage site
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2405-Tier1-PhCMV-TEV
Plasmid#169500PurposeTier-1 vector encoding PhCMV-driven tabacco etch virus protease TEV (PhCMV-TEV-pA).DepositorInsertPCMV-driven tobacco etch virus protease
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1023-Tier1-PhCMV-p2A-PuroR
Plasmid#169497PurposeTier-1 vector encoding PhCMV-driven p2A sequence fused to a marker for puromycine selection (PhCMV-p2A-PuroR-pA).DepositorInsertPCMV-driven P2A-PuroR
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH9-Tier1-PhCMV-3xNLS
Plasmid#169478PurposeTier-1 vector encoding PhCMV-driven triple nuclear localization signal expression (PhCMV-3xNLS-pA).DepositorInsertPCMV-driven nuclear localization signal
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable SinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PLXNC1
Plasmid#25312PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPLXNC1:T1198-K1305 (PLXNC1 Human)
UseTagsHisExpressionBacterialMutationContains amino acids T1198-K1305PromoterAvailable SinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTS2387-Tier1-PhCMV-p2a_mTagBFP2
Plasmid#169524PurposeTier-1 vector encoding PhCMV-driven blue fluorescent protein mTagBFP2 fused to a "self-cleaving" peptide p2a (PhCMV-p2a_mTagBFP2-pA).DepositorInsertPCMV-driven P2A-fused monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
UseTagsExpressionBacterialMutationPromoterAvailable SinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BLU-VIPR
Plasmid#220497PurposeRetroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBLU-VIPR
Plasmid#220498PurposePlasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).DepositorTypeEmpty backboneUseCRISPR; TransposonTagsExpressionMammalianMutationPromoterAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
gWiz_BM40-vGAT-VL-hIgG1-CL
Plasmid#218437Purposemammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domainDepositorInsertvGAT-VL-hIgG1-CL
UseAffinity Reagent/ AntibodyTagsBM40ExpressionMammalianMutationPromoterCMVAvailable SinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 rmtB
Plasmid#112880PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 ermC
Plasmid#112884PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInsertermC (ermM Staphylococcus epidermis)
UseTags6xHisExpressionBacterialMutationPromoterPlacAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 cat
Plasmid#112888PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertcat (Brevibacillus brevis) (cat Brevibacillus brevis)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVH64-Tier1-PSV40-Citrine_p2a_SEAP
Plasmid#169558PurposeTier-1 vector encoding PSV40-driven Citrine-p2A-SEAP expression (PSV40-Citrine-p2A-SEAP-pA).DepositorInsertPSV40-driven citrine-P2A-SEAP fusion construct
UseTagsExpressionMammalianMutationPromoterPSV40Available SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Chx10BP-LCL-EGFP
Plasmid#73992PurposeCre-dependent reporter plasmid for bipolar cells in the retinaDepositorInsertChx10BP-loxP-mCherry-loxP-EGFP
UseCre/LoxTagsExpressionMammalianMutationPromoterChx10BP (chx10 enhancer)Available SinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-Otx2-IRES-GFPd2
Plasmid#73996PurposePlasmid expressing Otx2(isoform b)-IRES-GFPd2DepositorAvailable SinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2396-Tier1-PhCMV-4xCOX8_Ypet
Plasmid#169534PurposeTier-1 vector encoding PhCMV-driven yellow fluorescent protein YPet localized to the mitochondria (PhCMV-4xCOX8-YPet-pA).DepositorInsertPCMV-driven miochondria-localized yellow fluorescent protein Ypet
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
Oct4-Cr1
Plasmid#66989PurposeExpresses gRNA that targets stop codon for OCT4DepositorInsertgRNA for Crispr targeting the OCT4 stop codon
UseTagsExpressionMammalianMutationPromoterAvailable SinceJuly 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK3-SDHAF3
Plasmid#136013PurposeSDHAF3 3'UTR (NM_020186.2) inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmidDepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only