Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,049 results for: golden gate

Clear keywords
Showing: 1481 - 1500 of 5049 results

  1. T17m

    Plasmid
    #121036
    Purpose
    MoClo golden gate assembly DE part L3S2P11 (262x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S2P11 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121036-plasmid-map-sequence-id-245428

    Enlarge

  2. T12m

    Plasmid
    #121031
    Purpose
    MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120029600 (strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121031-plasmid-map-sequence-id-249041

    Enlarge

  3. (P4)RBSS2

    Plasmid
    #251377
    Purpose
    RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S2
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251377-plasmid-map-sequence-id-503740

    Enlarge

  4. (P6)RBSS2

    Plasmid
    #251387
    Purpose
    RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S2
    Expression
    Bacterial
    Available Since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    251387-plasmid-map-sequence-id-503742

    Enlarge

  5. (P3)Adh

    Plasmid
    #251375
    Purpose
    Gene encoding aldehyde reductase slr1192 (Synechocystis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    adh
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251375-plasmid-map-sequence-id-503747

    Enlarge

  6. (P2)RBSS2

    Plasmid
    #251367
    Purpose
    RBS from C-phycocyanin beta gene in Synechococcus sp. PCC 7002
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS S2
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251367-plasmid-map-sequence-id-503730

    Enlarge

  7. (P5)pdc

    Plasmid
    #251385
    Purpose
    Gene encoding pyruvate decarboxylase (Zymomonas mobilis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    pdc
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251385-plasmid-map-sequence-id-503707

    Enlarge

  8. (P7)Pk

    Plasmid
    #251395
    Purpose
    Gene encoding pyruvate kinase (Enterococcus faecalis)
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    Pyruvate kinase
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251395-plasmid-map-sequence-id-503705

    Enlarge

  9. (P4)RBSB

    Plasmid
    #251383
    Purpose
    RBS designed in silico [75] for high expression in E. coli
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS B
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251383-plasmid-map-sequence-id-503702

    Enlarge

  10. (P2)RBSB

    Plasmid
    #251373
    Purpose
    RBS designed in silico [75] for high expression in E. coli
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS B
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251373-plasmid-map-sequence-id-503677

    Enlarge

  11. (P6)RBSB

    Plasmid
    #251393
    Purpose
    RBS designed in silico [75] for high expression in E. coli
    Depositor
    Kate Adamala, Pauli Kallio
    Insert
    RBS B
    Expression
    Bacterial
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    251393-plasmid-map-sequence-id-503674

    Enlarge

  12. pKS1603

    Plasmid
    #239527
    Purpose
    Express CpPT1(52-408) membrane enzyme cargo and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    2xCrk_SH3-mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239527-plasmid-map-sequence-id-479035

    Enlarge

  13. pVL1173

    Plasmid
    #239536
    Purpose
    Express CpPT1 membrane protein cargo with N-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker
    Depositor
    John Dueber
    Insert
    mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239536-plasmid-map-sequence-id-479033

    Enlarge

  14. pKS1616

    Plasmid
    #239547
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239547-plasmid-map-sequence-id-479031

    Enlarge

  15. pKS1615

    Plasmid
    #239546
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available Since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239546-plasmid-map-sequence-id-478363

    Enlarge

  16. pLII-CRISPR for CKA4

    Plasmid
    #238521
    Purpose
    Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha4
    Depositor
    Michael Hothorn
    Insert
    AtCK2alpha4 (AT2G23070 Mustard Weed)
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238521-plasmid-map-sequence-id-477173

    Enlarge

  17. pVL1172

    Plasmid
    #239535
    Purpose
    Express CpPT1 membrane protein cargo with C-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker
    Depositor
    John Dueber
    Insert
    CpPT1(52-408)-mTurquoise2
    Expression
    Yeast
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239535-plasmid-map-sequence-id-476352

    Enlarge

  18. pKS1575

    Plasmid
    #239523
    Purpose
    Express Vba2p membrane protein cargo fused directly to Pex22 TMD, peroxisomal Ynd1p truncation, and peroxisomal mRuby2
    Depositor
    John Dueber
    Insert
    ScPex22(1-36)-ScVba2p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239523-plasmid-map-sequence-id-475887

    Enlarge

  19. pKS1613

    Plasmid
    #239531
    Purpose
    Express CpPT1 membrane protein cargo fused to PTS1 and Pex22(1-36)-mRuby2; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    mTurquoise2-CpPT1(52-408)-SKL
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239531-plasmid-map-sequence-id-475886

    Enlarge

  20. pVL1281

    Plasmid
    #239540
    Purpose
    Express CDT1 membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    NcCDT1-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    239540-plasmid-map-sequence-id-475863

    Enlarge

Showing: 1481 - 1500 of 5049 results