We narrowed to 13,017 results for: SHI

1. WT1 KI gRNA2

   Plasmid

   #92313

   Purpose

   CRISPR-GFP-gRNA for cutting WT1

   Depositor

   Sean Palecek

   Article

   Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

   Insert

   WT1 (WT1 Human)
   

   Use

   Tags

   CRISPR GFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV_Actb MMEJ donor

   Plasmid

   #97315

   Purpose

   MMEJ donor for fusing a p2A-mCherry reporter to mouse Actb.

   Depositor

   Hui Yang

   Article

   Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

   Insert

   Actb MMEJ donor
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-BRD9_dBD-PGK-Puro-IRES-GFP

   Plasmid

   #75115

   Purpose

   Retroviral expression plasmid encoding a human BRD9 mutant lacking the bromodomain

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   deleted bromodomain

   Promoter

   Available since

   May 19, 2016

   Availability

   Academic Institutions and Nonprofits only

4. MSCV-Cterm_3xFL_BRD9-PGK-Puro-IRES-GFP

   Plasmid

   #75122

   Purpose

   Retroviral expression plasmid encoding wild type human BRD9 with a C terminal 3x FLAG-tag

   Depositor

   Christopher Vakoc

   Article

   Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

   Insert

   BRD9 (BRD9 Human)
   

   Use

   Retroviral

   Tags

   3x FLAG-tag

   Expression

   Mutation

   Promoter

   Available since

   May 23, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pET22-His-TEV-DOT1L-MM

   Plasmid

   #89611

   Purpose

   Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

   Depositor

   Leemor Joshua-Tor

   Article

   Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

   Insert

   Dot1L VVEL293MM Mutant (DOT1L Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA 3.3 ss-V5-hFZD4 (aa 37-537)

   Plasmid

   #115787

   Purpose

   Expresses human FZD4 with N-terminal V5-tag in mammalian cells, contains artificial signal sequence upstream of tag.

   Depositor

   Harald Junge

   Article

   Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

   Insert

   FZD4 (FZD4 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

   Plasmid

   #176459

   Purpose

   Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV

   Depositor

   Hui Yang

   Article

   Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/

   Insert

   HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEN25-Ub Hs

   Plasmid

   #83232

   Purpose

   pHis-par2-Ub Hs

   Depositor

   Gali Prag

   Article

   Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

   Insert

   Ubiqutin (UBC Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-Myc-PGK-Puro-IRES-GFP

    Plasmid

    #75124

    Purpose

    Retroviral expression plasmid encoding wild type murine Myc

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    myelocytomatosis oncogene (Myc Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only