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EGFP-NIP45 delta-SLD1

Plasmid

#210264

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD1. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-SLD1 (delta aa 260-335) (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

delta-SLD1

Promoter

CMV/TetO2

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 D394R

Plasmid

#210265

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N1

Plasmid

#210268

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N1 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N1 (aa 208-419) siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

delta-N1, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-N2

Plasmid

#210269

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-N2 resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-N2 (aa 261-419) siRES (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

delta-N2, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-NIP45 D394R

Plasmid

#210267

Purpose

Inducible expression of N-terminally mCherry-tagged NIP45 D394R resistant to siRNA. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 D394R siRES (NFATC2IP Synthetic)

Tags

mCherry

Expression

Mammalian

Mutation

D394R, siRES

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-NIP45 delta-SLD2

Plasmid

#210263

Purpose

Inducible expression of N-terminally EGFP-tagged NIP45 delta-SLD2. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Insert

NIP45 delta-SLD2 (aa 1-343) (NFATC2IP Synthetic)

Tags

EGFP

Expression

Mammalian

Mutation

delta-SLD2

Promoter

CMV/TetO2

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-AMOT p130∆PPxY2

Plasmid

#166712

Purpose

expresses AMOT with inactivating mutation in second PPxY motif in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 P262A P263A (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

changed proline 262 to alanine, proline 263 to al…

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3HA-AMOT p130∆PPxY3

Plasmid

#166713

Purpose

expresses AMOT with inactivating mutation in third PPxY motif in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

Angiomotin p130 P3072A P308A (AMOT Human)

Tags

2xHA

Expression

Mammalian

Mutation

changed proline 307 to alanine, proline 308 to al…

Promoter

CMV promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-Lyn-AktAR2

Plasmid

#125197

Purpose

SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2 variant of FRET-based AKT activity reporter

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

Lyn-AktAR2

Use

Transposon

Tags

Lyn tag (GCIKSKRKD), mCerulean3 and cpVenus[E172]

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSIREN-RetroQ-HSPE-sh1

Plasmid

#92033

Purpose

pSIREN-RetroQ vector containing shRNA sequence to HPSE

Depositor

Joe Landry

Article

Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

Insert

shRNA #1 to HPSE (Hpse Mouse)

Use

Retroviral

Available Since

July 19, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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