We narrowed to 19,449 results for: Tec

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SpCas9-NRRH

Plasmid

#198540

Purpose

Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRRH

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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SpCas9-NRCH

Plasmid

#198542

Purpose

Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NRCH

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-NS3/4A S139A

Plasmid

#186885

Purpose

Doxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

HCV NS3/4A protease with S139A mutation

Use

Retroviral

Mutation

S139A

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pTRE3G-WHG cGAS-HA

Plasmid

#186867

Purpose

Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

White-handed gibbon cGAS with C-terminal HA tag

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pYAMTr2GCsgSc-SeLEU2

Plasmid

#224871

Purpose

Disruption of LEU2 genes in the Saccharomyces sense strict group species

Depositor

Katsunori Suzuki

Article

Kiyokawa et al Appl Microbiol Biotechnol. 2024 Jul 12;1

Insert

pYAMTr2GC having the guide sequence Sc-SeLEU2 (LEU2, DI49_0736 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_CBS-FRT-OCS-BeYDVRepRepA-tNos (GB4646)

Plasmid

#225438

Purpose

Transcriptional unit for copper- and flipase-regulated BeYDV Rep/RepA. OCSt, flanked by FRT sites, is embedded in the 5'UTR of miniDFR.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

CBS-FRT-OCS-BeYDVRepRepA-tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha2_BeYDV geminino 1.0 (GB4480)

Plasmid

#225436

Purpose

BeYDV LIR1:2nd intron+eGFP CDS+Ter35S+p35S+1st intron:LIR1 in alpha2. Deconstructed replicon that needs BeYDV Rep for circularization and, thus, replication and eGFP transcription.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

BeYDV geminino 1.0

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)

Plasmid

#225437

Purpose

Transcriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

CBS-att-OCS-BeYDVRep/RepA-tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR-FRT-OCSterm-FRT-miniDFR (GB4642)

Plasmid

#225430

Purpose

OCS terminator flanked by two flippase recognition target (FRT) sites located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-FRT-OCSterm-FRT-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUPD2_miniDFR-att-OCSterm-att-miniDFR (GB4641)

Plasmid

#225431

Purpose

OCS terminator flanked by two att sites of PhiC31 integrase located at position 112 of the SlDFR minimal promoter

Depositor

Diego Orzaez

Article

Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

Insert

miniDFR-att-OCSterm-att-miniDFR

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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