Addgene

We narrowed to 10,494 results for: nar;

Showing: 1521 - 1540 of 10494 results

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pET42a-lambdaN+-L+-GSH

Plasmid

#98894

Purpose

Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method

Depositor

Pascale Legault

Article

Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

Insert

lambdaN+-L+

Use

Tags

GST tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pRRL-GFPGO2(NGG)-PGK-Neo

Plasmid

#136891

Purpose

Lentivirus for base editing activatable GFP expression in mammalian cells

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

GFPGO2(NGG)

Use

Lentiviral

Tags

2xNLS

Expression

Mutation

ATG>ACG

Promoter

SFFV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJK506

Plasmid

#155382

Purpose

dCas12a (D917A) + PA4-mVenus. (removed crRNAs)

Depositor

Pamela Silver

Article

Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

Inserts

dCas12a (F. novicida)
PA4-mVenus

Use

CRISPR

Tags

Expression

Bacterial

Mutation

D917A (nuclease-deactivating)

Promoter

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pRG710_lexO-HO_LexA-TF_HIS3MX

Plasmid

#154819

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pNG1

Plasmid

#30985

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat NG1

Use

Tags

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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SGL40C.EFS.E2Crimson

Plasmid

#100894

Purpose

Lentiviral gRNA delivery

Depositor

Dirk Heckl

Article

Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

Insert

E2Crimson

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pFUS_B10

Plasmid

#31027

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(0)_T2AGAL4

Plasmid

#125211

Purpose

Artificial phase 0 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Expression

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(2)_T2AGAL4

Plasmid

#125213

Purpose

Artificial phase 2 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Expression

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGAL4

Plasmid

#125212

Purpose

Artificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Expression

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGIGYF_AH

Plasmid

#148918

Purpose

Insect Expression of DmGIGYF

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

DmGIGYF (Gyf Fly)

Use

Tags

Expression

Insect

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pNUT3

Plasmid

#175744

Purpose

Single-component photo-inducible (CRY2-based) nucleolus-targeting tool 3

Depositor

Yubin Zhou

Article

Tan et al Nucleic Acids Res. 2022 Mar 23. pii: 655

Insert

mCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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PBDGTV

Plasmid

#100859

Purpose

A piggyBac transposon based dual directional gene trap vector, in which, the mutagens are two non-selectable gene-trap cassettes in opposite orientations.

Depositor

Yue Huang

Article

Liu et al Nucleic Acids Res. 2017 Sep 28. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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LoriT

Plasmid

#163691

Purpose

Modified L4440 containing now CamR and oriT for conjugation

Depositor

Baris Tursun

Article

Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

Insert

oriT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pHD1

Plasmid

#30974

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

repeat HD1

Use

Tags

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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AB65_pCAG.Cas9-D10A.rBGpA

Plasmid

#199256

Purpose

Expression construct encoding the SpCas9-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SpCas9.D10A nickase

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pFUS_A

Plasmid

#31028

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Use

Tags

Expression

Mutation

Promoter

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–CgRNA

Plasmid

#64955

Purpose

Control gRNA (GTCAAGGCACTCTTGCCTA)

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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pJEC581

Plasmid

#174369

Purpose

RFP reporter used to evaluate CRISPRa in bacteria, contains gRNA target sites every 10 bp upstream of the promoter.

Depositor

James Chappell

Article

Villegas Kcam et al Nucleic Acids Res. 2021 May 7;49(8):4793

Insert

RFP with PAM rich sequence upstream promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23117

Available since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCas9–mKate2ps–T1gRNA

Plasmid

#62717

Purpose

t1 gRNA (ATGAGAATCAAGGCGGTCGA)

Depositor

Leonidas Bleris

Article

Moore et al Nucleic Acids Res. 2015 Jan 30;43(2):129

Insert

Cas9–mKate2ps

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 29, 2015

Availability

Academic Institutions and Nonprofits only

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