Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 6,904 results for: crispr cas9 plasmids

Showing: 771 - 780 of 6904 results

  1. pCMV-T7-SpCas9(MRKCLN)-P2A-EGFP (AHK130)

    Plasmid
    #223091
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRKCLN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MRKCLN)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MRKCLN)=D1135M, S1136R, G1218K, E1219C, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223091-plasmid-map-sequence-id-447668

    Enlarge

  2. pCMV-T7-SpCas9(LWKFEG)-P2A-EGFP (RTW3931)

    Plasmid
    #223092
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKFEG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(LWKFEG)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWKFEG)=D1135L, S1136W, G1218K, E1219F, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223092-plasmid-map-sequence-id-451167

    Enlarge

  3. pCMV-T7-SpCas9(CWNWNS)-P2A-EGFP (RAS3011)

    Plasmid
    #223093
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with CWNWNS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(CWNWNS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(CWNWNS)=D1135C, S1136W, G1218N, E1219W, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223093-plasmid-map-sequence-id-447647

    Enlarge

  4. pCMV-T7-SpCas9(LWRYEK)-P2A-EGFP (RAS2986)

    Plasmid
    #223094
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(LWRYEK)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWRYEK)=D1135L, S1136W, G1218R, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223094-plasmid-map-sequence-id-447661

    Enlarge

  5. pCMV-T7-SpCas9(LWKYSS)-P2A-EGFP (RAS3024)

    Plasmid
    #223080
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWKYSS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(LWKYSS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWKYSS)=D1135L, S1136W, G1218K, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223080-plasmid-map-sequence-id-447659

    Enlarge

  6. pCMV-T7-SpCas9(MWKYQA)-P2A-EGFP (RAS2965)

    Plasmid
    #223081
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MWKYQA amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MWKYQA)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MWKYQA)=D1135M, S1136W, G1218K, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223081-plasmid-map-sequence-id-447674

    Enlarge

  7. pCMV-T7-SpCas9(LWQYQH)-P2A-EGFP (RAS2885)

    Plasmid
    #223082
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWQYQH amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(LWQYQH)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWQYQH)=D1135L, S1136W, G1218Q, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223082-plasmid-map-sequence-id-447660

    Enlarge

  8. pCMV-T7-SpCas9(RQMYQG)-P2A-EGFP (RAS3009)

    Plasmid
    #223083
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with RQMYQG amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(RQMYQG)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(RQMYQG)=D1135R, S1136Q, G1218M, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223083-plasmid-map-sequence-id-447686

    Enlarge

  9. pCMV-T7-SpCas9(MWKYQS)-P2A-EGFP (RAS2898)

    Plasmid
    #223084
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(MWKYQS)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(MWKYQS)=D1135M, S1136W, G1218K, E1219Y, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223084-plasmid-map-sequence-id-447682

    Enlarge

  10. pCMV-T7-SpCas9(LWSSLY)-P2A-EGFP (RAS2997)

    Plasmid
    #223085
    Purpose
    pCMV and pT7 Human expression plasmid for SpCas9 enzyme with LWSSLY amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(LWSSLY)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(LWSSLY)=D1135L, S1136W, G1218S, E1219S, R1…
    Promoter
    CMV and T7
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223085-plasmid-map-sequence-id-447663

    Enlarge

Showing: 771 - 780 of 6904 results