Addgene

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pDL6

Plasmid

#175888

Purpose

pENTRY for Gateway LR reaction

Depositor

Nemo Peeters

Article

Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

Insert

ID#9

Use

Pentry clone

Tags

Expression

Mutation

Promoter

Available since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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CDS_mTurqcp_chlo

Plasmid

#163936

Purpose

L0 part - CDS

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

FP optimised for Marchantia polymorpha chloroplast expression, from Genbank ID KT364744

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pFLinkC-AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV

Plasmid

#137117

Purpose

Protease Switch Clone ID: 2-A5

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMV

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFLinkC-AI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV

Plasmid

#137115

Purpose

Protease Switch Clone ID: 1-A9

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFLinkC-AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV

Plasmid

#137116

Purpose

Protease Switch Clone ID: 1-C9

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-SnoopCatcher

Plasmid

#72322

Purpose

Bacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a-SnoopCatcher

Use

Tags

His6 tag

Expression

Bacterial

Mutation

changed G842T and D848G of S. pneumoniae adhesin …

Promoter

T7

Available since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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mc2 puro

Plasmid

#206215

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon hPGK

Plasmid

#206230

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR15.2-2H12.D11opt

Plasmid

#221185

Purpose

cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter (Internal Lab ID: UJ11239)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2.1

Use

Tags

Expression

Bacterial

Mutation

Promoter

Constitutive

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-GEMSgRNA2

Plasmid

#204768

Purpose

pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

guide RNA targeting GEMS m6A sensor mRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pQFmcs-2H12.D11

Plasmid

#221183

Purpose

cdGreen2 expressed from a cumate-inducible promoter (Internal Lab ID: UJ11216)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Use

Tags

Expression

Bacterial

Mutation

Promoter

PQ5 (cumate-inducible)

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-2H12

Plasmid

#221186

Purpose

His6-tagged cdGreen expressed from T7 promoter (Internal Lab ID: UJ11308)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEM047 [T7-assisted CROPseq sgRNA expression vector]

Plasmid

#224899

Purpose

Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA

Depositor

Thomas Norman

Article

Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.

Inserts

eGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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mc2 puro EF1a

Plasmid

#206221

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon EF1a

Plasmid

#206224

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p2H12Matry

Plasmid

#221143

Purpose

cdGreen-Matry expressed from an aTc-inducible promoter (Internal Lab ID: UJ11208)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen-Matry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-2H12.D11

Plasmid

#221145

Purpose

His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon

Plasmid

#206218

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 blast

Plasmid

#206217

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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p2H12Matry-blind

Plasmid

#221181

Purpose

cdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Insert

cdGreen-Matry(blind)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Ptet

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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