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pLNHA-C1-HsNot1iso1-del1097-1110-shRNAres_W

Plasmid

#147882

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110-shRNAres

Depositor

Elisa Izaurralde

Publication

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-ATG101-W110D/F112D

Plasmid

#188106

Purpose

Expresses human ATG101 HORMA W110D/F112D mutant in mammalian cells

Depositor

James Hurley

Publication

Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

Insert

ATG101 (ATG101 Human)

Tags

GST tag

Expression

Mammalian

Mutation

codon-optimized ATG101-W110D/F112D

Promoter

CMV

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPAN2-F1103A-dsRNAres_Y

Plasmid

#148075

Purpose

Insect Expression of DmPAN2-F1103A-dsRNAres

Depositor

Elisa Izaurralde

Publication

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-F1103A-dsRNAres (PAN2 Fly)

Expression

Insect

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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PCx35-pcDNA-S110A, S276A, S298ter

Plasmid

#42927

Depositor

John O'Brien

Publication

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Expression

Mammalian

Mutation

Ser110 to Ala, Ser276 to Ala, and Ser298 to stop …

Promoter

CMV

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-delta848-918

Plasmid

#238313

Purpose

ScVip1-536-1107-delta848-918 protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-delta848-918

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-ScEap1p_107-122-Y107IA108KS110T-GB1_AG

Plasmid

#148813

Purpose

Bacterial Expression of ScEap1p_107-122-Y107IA108KS110T

Depositor

Elisa Izaurralde

Publication

Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

Insert

ScEap1p_107-122-Y107IA108KS110T (EAP1 Budding Yeast)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmUPF1_1-1106-siRNAres-V5His6_N

Plasmid

#146985

Purpose

Insect Expression of DmUPF1_1-1106-siRNAres

Depositor

Elisa Izaurralde

Insert

DmUPF1_1-1106-siRNAres (Upf1 Fly)

Expression

Insect

Mutation

1 non silent mutations: G995E compared to the seq…

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmUPF1_92-1106-siRNAres-V5His6_N

Plasmid

#146988

Purpose

Insect Expression of DmUPF1_92-1106-siRNAres

Depositor

Elisa Izaurralde

Insert

DmUPF1_92-1106-siRNAres (Upf1 Fly)

Expression

Insect

Mutation

1 non silent mutations: G995E compared to the seq…

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA_X

Plasmid

#147999

Purpose

Mammalian Expression of HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA

Depositor

Elisa Izaurralde

Publication

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_840-1605-del1097-1110-IKGRQtoDSYAA (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1-del1097-1110-R1138A-shRNAres_W

Plasmid

#147881

Purpose

Mammalian Expression of HsNot1iso1-del1097-1110-R1138A-shRNAres

Depositor

Elisa Izaurralde

Publication

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-del1097-1110-R1138A-shRNAres (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HsNot1iso1_840-1605-del1097-1110-RNFtoAAA_X

Plasmid

#148000

Purpose

Mammalian Expression of HsNot1iso1_840-1605-del1097-1110-RNFtoAAA

Depositor

Elisa Izaurralde

Publication

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1_840-1605-del1097-1110-RNFtoAAA (CNOT1 Human)

Expression

Mammalian

Mutation

1 silent mutation compared to the sequence of iso…

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pKP110 [pRS416-YBR139Wp-YBR139W(N163,242Q)-PA-ADH1t]

Plasmid

#106462

Purpose

Expresses Atg42/Ybr139w(N163,242Q) with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Publication

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(N163,242Q)

Tags

PA

Expression

Bacterial and Yeast

Mutation

changed Asparagines at positions 163 and 242 to G…

Promoter

YBR139W

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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Plasmid 1 (AAV-Hpd1-GOI, VectorBuilder, VB211001-1008sqe)

Plasmid

#258683

Purpose

knockout GOI and overexpress another GOI in cells/animals

Depositor

Stan van de Graaf

Publication

Wu et al Mol Ther Adv. 2026 Mar 18;34(2):201720.

Insert

4-hydroxyphenylpyruvate dioxygenase (Hpd Mouse)

Use

AAV and CRISPR

Promoter

U6

Available since

Oct. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pMW96_6xHis-Sumo-TEV-HsKIF1C(886-1103)-mNG

Plasmid

#253319

Purpose

bacterial expression and purification of mNG-tagged IDR domain of human KIF1C (aa 886-1103)

Depositor

Kristen Verhey

Publication

Geng et al EMBO J. 2024 Aug;43(15):3192-3213. doi:

Insert

HsKIF1C(886-1103) (KIF1C Human)

Tags

His-SUMO and mNeonGreen

Expression

Bacterial

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pMW96_6xHis-Sumo-TEV-HsKIF1C(785-1103)-mNG

Plasmid

#253318

Purpose

bacterial expression and purification of mNG-tagged CC4+IDR domains of human KIF1C (aa 785-1103)

Depositor

Kristen Verhey

Publication

Geng et al EMBO J. 2024 Aug;43(15):3192-3213. doi:

Insert

HsKIF1C(785-1103) (KIF1C Human)

Tags

His-SUMO and mNeonGreen

Expression

Bacterial

Available since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-SpCas9-VRQR(+G1104K)-P2A-EGFP (LRF54)

Plasmid

#242671

Purpose

CMV promoter expression plasmid for human codon optimized SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Depositor

Benjamin Kleinstiver

Publication

Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415

Insert

pCMV-SpCas9-VRQR(G1140K)-BPNLS(SV40)-3xFLAG-P2A-EGFP

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

VRQR + G1104K mutations in SpCas9(G1104K/D1135V/G…

Promoter

CMV and T7

Available since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A-H548A-R551A

Plasmid

#238317

Purpose

ScVip1-536-1107-R547A-H548A-R551A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A-H548A-R551A

Available since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K554A-K556A-K644A

Plasmid

#238328

Purpose

ScVip1-536-1107-K554A-K556A-K644A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A-K556A-K644A

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-P553V-G646V-G647A

Plasmid

#238322

Purpose

ScVip1-536-1107-P553V-G646V-G647A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-P553V-G646V-G647A

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K817S-D819A-S821A

Plasmid

#238326

Purpose

ScVip1-536-1107-K817S-D819A-S821A protein expression in insect cells

Depositor

Michael Hothorn

Publication

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K817S-D819A-S821A

Available since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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