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PMT-mApple_2

Plasmid

#203772

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Publication

Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

Insert

PMT-mApple dimer

Expression

Mammalian

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pWT007a

Plasmid

#107889

Purpose

W1.0.1 plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.1

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-Cas9, PLac-sgRNA1

Expression

Bacterial

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pWT047c

Plasmid

#107903

Purpose

W2.7-1 plasmid. Contains PR-sd5-BE2, PLac-sgRNA1 and is part of CAMERA 2.7

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PR-sd5-BE2, PLac-sgRNA1

Expression

Bacterial

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT045c

Plasmid

#107902

Purpose

W2.6 plasmid. Contains PTetO-SD8-BE2, PPSP-sgRNA1 and is part of CAMERA 2.6

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PPSP-sgRNA1

Expression

Bacterial

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT007g

Plasmid

#107890

Purpose

W1.0.2 plasmid. Contains PTetO-sd2-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.2

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2-Cas9, PLac-sgRNA1

Expression

Bacterial

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT007h

Plasmid

#107892

Purpose

W1.0.1c plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA(con.) and is part of CAMERA 1.0.1c

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-Cas9, PLac-sgRNA(con.)

Expression

Bacterial

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT060b

Plasmid

#107904

Purpose

W2m.0-2 plasmid. Contains U6-sgRNA A (CCR5) and is part of a system for CAMERA 2.5

Depositor

David Liu

Publication

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

U6-sgRNA A (CCR5) (CCR5 Human)

Expression

Mammalian

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pPV-EF1a-EGxxFP-iP-A

Plasmid

#204624

Purpose

Cloning vector for SSA vector (EGxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

Depositor

Akitsu Hotta

Publication

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Expression

Mammalian

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_C2517

Plasmid

#91086

Purpose

Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

TaU3

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_C2520

Plasmid

#91089

Purpose

Module C, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Promoter

OsU6

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2515b

Plasmid

#91073

Purpose

Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

AtU6

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRoTnsABCDQ

Plasmid

#178772

Purpose

Expression of RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

Depositor

Chase Beisel

Publication

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

RotnsAB, RotnsC, RotnsD, RotniQ from Rippkaea orientalis

Expression

Bacterial

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRoDonor

Plasmid

#178773

Purpose

Expression of RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

Depositor

Chase Beisel

Publication

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

RoCAST left end, cam, RoCAST right end from Rippkaea orientalis

Expression

Bacterial

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-PacI

Plasmid

#170100

Purpose

deGFP reporter plasmid with unique PacI restriction enzyme recognition site

Depositor

Chase Beisel

Publication

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEc-crRNA1

Plasmid

#170088

Purpose

encodes E. coli type I-E single spacer array

Depositor

Chase Beisel

Publication

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E single spacer array

Use

CRISPR and Synthetic Biology

Promoter

J23119

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas7

Plasmid

#170092

Purpose

expresses E. coli type I-E Cas7

Depositor

Chase Beisel

Publication

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas7

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCas8

Plasmid

#170093

Purpose

expresses E. coli type I-E Cas8

Depositor

Chase Beisel

Publication

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cas8

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pEcCse2

Plasmid

#170094

Purpose

expresses E. coli type I-E Cse2

Depositor

Chase Beisel

Publication

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

E. coli type I-E Cse2

Tags

His

Expression

Bacterial

Promoter

T7

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_dimer_mKate2

Plasmid

#167333

Purpose

Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells

Depositor

Tom Misteli

Publication

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Changed K95A, Y99F, E106A, E112A and R116A

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH_CMV_HMGA_mKate2

Plasmid

#167334

Purpose

Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells

Depositor

Tom Misteli

Publication

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

TFAM (TFAM Human)

Use

Lentiviral

Tags

mKate2

Expression

Mammalian

Mutation

Truncation mutant containing MTS (1-42) and HMGA …

Promoter

CMV

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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