We narrowed to 9,789 results for: Coli

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hSRAP.TWIN1.a13-215

Plasmid

#49817

Purpose

expression vector for SRAP(13-215) in pTWIN1 vector

Depositor

Thomas Cech

Article

McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

Insert

steroid receptor RNA activator protein aa 13-215 (SRA1 Human)

Tags

intein & chitin binding domain

Expression

Bacterial

Mutation

wildtype codon-optimized for E. coli expression

Promoter

T7

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pSTTa-GPA1-S52N

Plasmid

#248649

Purpose

Vector for protein expression of 2x StrepII-tagged Arabidopsis heterotrimeric G protein GPA1-S52N ("inactive" mutant) in E. coli.

Depositor

Sarah Assmann

Article

Gookin et al Front Mol Biosci. 2025 Apr 7;12:1513660.

Insert

GPA1 (GP ALPHA 1 Mustard Weed)

Tags

2x StrepII, Thrombin site, TEV site

Expression

Bacterial

Mutation

S52N

Promoter

tac

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pSTTa-GPA1-Q222L

Plasmid

#248650

Purpose

Vector for protein expression of 2x StrepII-tagged Arabidopsis heterotrimeric G protein GPA1-Q222L ("constitutively active" mutant) in E. coli.

Depositor

Sarah Assmann

Article

Gookin et al Front Mol Biosci. 2025 Apr 7;12:1513660.

Insert

GPA1 (GP ALPHA 1 Mustard Weed)

Tags

2x StrepII, Thrombin site, TEV site

Expression

Bacterial

Mutation

Q222L

Promoter

tac

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pD441-H6-SUMO-HyPerFLEX

Plasmid

#216676

Purpose

Plasmid with cleavable His-tag for expression of the HyPer-FLEX sensor for hydrogen peroxide in E. coli

Depositor

Joris Messens

Article

Potekhina et al Nat Chem Biol. 2025 Oct 16. doi: 10.1038

Insert

HyPerFLEX

Tags

6xHis-SUMO

Expression

Bacterial

Promoter

T5

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLIC-His6-MBP-TEV-Rgs14

Plasmid

#236395

Purpose

Expresses His6-MBP-TEV-Rgs14 in E. coli

Depositor

John Hepler

Article

Brown et al J Biol Chem. 2015 Apr 3;290(14):9037-49.

Insert

Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)

Tags

hexahistidine (H6) tag and maltose-binding protei…

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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WT USP7 UBL-12

Plasmid

#227230

Purpose

Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Tags

6His

Expression

Bacterial

Mutation

aa 535-775

Promoter

T7-promoter

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(7CNW)RS

Plasmid

#222874

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pQE30-DARPin-E11

Plasmid

#226784

Purpose

Expression of His-tagged DARPin E11 in E. coli

Depositor

Sebastian Mathea

Article

Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi

Insert

DARPin E11

Tags

FLAG and His

Expression

Bacterial

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanIV-Streptomyces

Plasmid

#226250

Purpose

Expresses precursor peptide and lanthipeptide synthetase from Streptomyces LanIV (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanIV-Streptomyces)

Tags

His6

Expression

Bacterial

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET28:LanII-2C

Plasmid

#225070

Purpose

Expresses precursor peptide and lanthipeptide synthetase from LanII-2C (FAST-RiPPs) in E. coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

Insert

LanA (LanII-2C)

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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