We narrowed to 2,687 results for: GUS

1. MOBE4

   Plasmid

   #219944

   Purpose

   Mammalian expression of MOBE4 protein (apt-CBE-SL3_PT2A_apt-ABE8.20)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-SL3_PT2A_apt-ABE8.20
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. apt-ABE8.20

   Plasmid

   #219938

   Purpose

   Mammalian expression of apt-ABE8.20 protein (TadA8.20_32aa-linker_Com)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   TadA8.20_32-aa_Com
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MOBE2

   Plasmid

   #219942

   Purpose

   Mammalian expression of MOBE2 protein (apt-CBE-3'end_PT2A_apt-ABE8.20)

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   apt-CBE-3'end_PT2A_apt-ABE8.20
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. nCas9-SpRY_P2A-mCherry

   Plasmid

   #219935

   Purpose

   Mammalian expression of SpRY-nCas9 with P2A-mCherry marker for use with MOBE systems

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Sp-nCas9(D10A)-SpRY_P2A-mCherry
   

   Use

   CRISPR

   Tags

   SV40 bpNLS and SV40 bpNLS-P2A-mCherry

   Expression

   Mammalian

   Mutation

   nCas9(D10A)

   Promoter

   CMV

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. apt-CBE-3'end

   Plasmid

   #219939

   Purpose

   Mammalian expression of apt-CBE-3'end protein (MCP_32aa-linker_evoAPOBEC1) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   MCP_32-aa_evo-rA1_2xUGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. nCas9-NG_P2A-mCherry

   Plasmid

   #219934

   Purpose

   Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Sp-nCas9(D10A)-NG_P2A-mCherry
   

   Use

   CRISPR

   Tags

   SV40 bpNLS and SV40 bpNLS-P2A-mCherry

   Expression

   Mammalian

   Mutation

   nCas9(D10A)

   Promoter

   CMV

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. apt-CBE-SL3

   Plasmid

   #219940

   Purpose

   Mammalian expression of apt-CBE-SL3 protein (MCP_16aa-linker_evoAPOBEC1) with P2A-EGFP marker

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   MCP_16-aa_evo-rA1_2xUGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HiFi-nCas9-NG_P2A-mCherry

   Plasmid

   #219936

   Purpose

   Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
   

   Use

   CRISPR

   Tags

   SV40 bpNLS and SV40 bpNLS-P2A-mCherry

   Expression

   Mammalian

   Mutation

   HiFi-nCas9(D10A/R691A)

   Promoter

   CMV

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SL3-MS2-gRNA GG backbone (MOBE3-4)

   Plasmid

   #219949

   Purpose

   Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   gRNA-MS2-SL3 (S. pyogenes Cas9)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 3'-com-gRNA GG backbone (MOBE1-4)

    Plasmid

    #219947

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-com-3'end (S. pyogenes Cas9)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 3'MS2-gRNA GG backbone (MOBE1-2)

    Plasmid

    #219948

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-3'end (S. pyogenes Cas9)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CROP-seq-puro-v2-no-scaffold

    Plasmid

    #228521

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Inserts

    Use

    Lentiviral

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CROP-seq-mTurquoise2-v2-no-scaffold

    Plasmid

    #228522

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Insert

    mTurquoise2
    

    Use

    Lentiviral

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CROP-seq-copGFP-v2-no-scaffold

    Plasmid

    #228520

    Purpose

    CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using copGFP as selection marker

    Depositor

    Jellert Gaublomme

    Article

    Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Fluorescent reporter for MOBE1-2

    Plasmid

    #219945

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE1-2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-3'end
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Fluorescent reporter for MOBE3-4

    Plasmid

    #219946

    Purpose

    Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    EGFP(A111V/L202S)

    Promoter

    CMV, U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PCS2+ Cas9

    Plasmid

    #122948

    Purpose

    For in vitro transcription to produce Cas9 mRNA for microinjection into mouse embryos

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Cas9
    

    Use

    Synthetic Biology

    Promoter

    SP6

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBSDONR P4r-P2

    Plasmid

    #121527

    Purpose

    Gateway Entry Vector for Multisite Gateway Cloning

    Depositor

    Roger Innes

    Article

    Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Sept. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EasyFusion Halo

    Plasmid

    #112850

    Purpose

    General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    HaloTag
    

    Use

    Synthetic Biology

    Promoter

    No

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EasyFusion mCherry

    Plasmid

    #112849

    Purpose

    General donor vector with mCherry coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Promoter

    No

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge