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Showing: 141 - 159 of 159 results

  5. FUW-crRNA-scrambled-U6_TRE-dLbCpf1-NLS-hCTCF_WT-HA-P2A-tagBFP

    Plasmid
    #194897
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-WT and scrambled crRNA without target in human genome.
    Depositor
    Shawn Liu
    Article
    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd
    Inserts
    dLbCpf1-NLS-hCTCF_WT
    U6-crRNA-scramble
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Availability
    Academic Institutions and Nonprofits only
    194897-plasmid-map-sequence-id-382810

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  6. TFORF2986

    Plasmid
    #144462
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MECP2 (MECP2 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    144462-plasmid-map-sequence-id-407343

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  7. TFORF0931

    Plasmid
    #143243
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MECP2 (MECP2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    143243-plasmid-map-sequence-id-396155

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  13. pX551-CMV-SpCas9

    Plasmid
    #107024
    Purpose
    AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    107024-plasmid-map-sequence-id-208524

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  15. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid
    #158576
    Purpose
    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.
    Depositor
    Rudolf Jaenisch
    Article
    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii
    Insert
    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    Use
    Tags
    V5 and HA on the endogenous KCC2 protein product
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    158576-plasmid-map-sequence-id-311917

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  16. dCas9-RPT6-FLAG

    Plasmid
    #205416
    Purpose
    Expresses dCas9-RPT6 with FLAG label
    Depositor
    Timothy Jarome
    Insert
    dCas9-RPT6 (Psmc5 Rat)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    205416-plasmid-map-sequence-id-405405

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Showing: 141 - 159 of 159 results