...mutations in the MECP2 gene leading to Rett Syndrome (Amir et al., 1999). We now know that MECP2 binds to methylated.... Loss of MECP2 in adult animal models results in rapid symptom onset, suggesting that MECP2 is also essential...parts of MECP2 influence Rett Syndrome. Zoghbi’s lab found that a small DNA binding motif in MECP2 interacts...different MECP2 mutations. More recently, the Bird lab found that only a portion of the MECP2 protein is... Rett Syndrome MECP2 follows the “Goldilocks” principle, in which too much of the MECP2 protein is as ... Figure 1: Schematic of the human MECP2 locus and MECP2 protein, showing mutations corrected by ...system to both precisely insert the MECP2-R270X mutation into the MECP2 gene in wild-type human iPSCs and...

...Publication PI Mecp2 NLucTom Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato...) OMIM MECP2 - MECP2 curated literature information (Link opens in a new window) UniProt MECP2 - MECP2... in the gene methyl-CpG binding protein 2 ( MECP2 ). MECP2 Rett syndrome is an X-linked disorder caused...half of cells expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these X inactivation...major function of MECP2 is to regulate gene expression either locally or globally. The MECP2 protein contains...include MECP2 null alleles or common mutations that recapitulate Rett syndrome in humans. MECP2 knockout...Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the MECP2 gene. Use...

... make dual guide expressing plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence.... Plasmids 175573 and 175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express... pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2 ( Addgene #110821 ) VPR ( Addgene #63798) Plasmids...

...codon-optimized SpCas9, driven by the truncated MeCP2 promoter (pAAV-pMecp2-SpCas9-spA) for expressing Cas9 in neurons...PX600; CMV-driven SaCas9 60957 : PX551; Truncated MeCP2 promoter-driven SpCas9; for neuronal expression ...

.../17R] TrpV1 Rat Mouse IgG2a 177518 Anti-MECP2 [N227/21R] MECP2 Human Mouse IgG2a 177519 Anti-Shank2 [N23B...