We narrowed to 211 results for: nano bret

1. pRset-ARSeNL

   Plasmid

   #207872

   Purpose

   Bacterial expression of the BRET ATP sensor

   Depositor

   Mathew Tantama

   Article

   Min et al Sensors (Basel). 2019 Aug 10;19(16). pii

   Insert

   ARSeNL
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GW1-mScarlet-Epac-GeNL

   Plasmid

   #207874

   Purpose

   Mammalian expression of the FRET/BRET cAMP sensor

   Depositor

   Mathew Tantama

   Article

   French et al ACS Omega. 2019 Sep 12;4(13):15504-15511

   Insert

   mScarlet-hEpac1(149-881)-mNeonGreen-NanoLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT7-CalfluxVTN

   Plasmid

   #83926

   Purpose

   Bioluminescent Reporter of Intracellular Calcium

   Depositor

   Carl Johnson

   Article

   Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

   Insert

   CalfluxVTN
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET20b-NLuc-MBP-6xHis

   Plasmid

   #135931

   Purpose

   Expresses NanoLuciferase in bacterial cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Use

   Tags

   MBP-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEF1a-T2-NLuc

   Plasmid

   #135938

   Purpose

   Expresses NanoLuciferase targeted to the mitochondira in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Use

   Tags

   T2 mitochondrial localization sequence

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEF1a-2xSV40_NLS-NLuc

   Plasmid

   #135953

   Purpose

   Expresses the NanoLuciferase targeted to the nucleus in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

   Insert

   NanoLuciferase
   

   Use

   Tags

   2xSV40-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AIMTOR T757

   Plasmid

   #140828

   Purpose

   AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells

   Depositor

   Anne Bonnieu

   Article

   Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

   Insert

   AIMTOR T757 (ULK1 Human)
   

   Use

   Tags

   NES Nuclear export signal

   Expression

   Mammalian

   Mutation

   The insert comprises only a small sequence of hum…

   Promoter

   Available since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRetroX-Tight-MCS_PGK-GpNLuc

   Plasmid

   #70185

   Purpose

   Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRetroX-Tight-MCS_PGK-OgNLuc

   Plasmid

   #70186

   Purpose

   Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HiBiT-FZD1

    Plasmid

    #195839

    Purpose

    Expresses human FZD1 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD1 (FZD1 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. G15-CASE

    Plasmid

    #168126

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein G15. Composed of the subunits G alpha 15 (GNA15) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNA15, GNB3, GNG9 (GNA15, GNB3, GNGT2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG9

    Expression

    Mammalian

    Mutation

    NLuc is inserted at E244/N245 within GNA15

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Gi3-CASE

    Plasmid

    #168122

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A114/E115 within GNAI1

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Gi1-CASE

    Plasmid

    #168120

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG2

    Expression

    Mammalian

    Mutation

    NLuc is inserted at A121/E122 within GNAI1

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HiBiT-FZD8

    Plasmid

    #195846

    Purpose

    Expresses human FZD8 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD8 (FZD8 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HiBiT-FZD7

    Plasmid

    #195845

    Purpose

    Expresses human FZD7 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD7 (FZD7 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HiBiT-FZD10

    Plasmid

    #195848

    Purpose

    Expresses human FZD10 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD10 (FZD10 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HiBiT-FZD5

    Plasmid

    #195843

    Purpose

    Expresses human FZD5 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD5 (FZD5 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HiBiT-FZD3

    Plasmid

    #195841

    Purpose

    Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD3 (FZD3 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    I292V and V495I

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HiBiT-FZD4

    Plasmid

    #195842

    Purpose

    Expresses human FZD4 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD4 (FZD4 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HiBiT-FZD2

    Plasmid

    #195840

    Purpose

    Expresses human FZD2 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD2 (FZD2 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge