Skip to main content
Addgene

We narrowed to 8,358 results for: BLI;

Showing: 3161 - 3200 of 8358 results
  1. phCMV-229E-D15

    Plasmid
    #188908
    Purpose
    express truncated 229E spike for pseudovirus production
    Depositor
    Insert
    229E spike (S 229E)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    c-terminal 15aa deletion
    Promoter
    CMV
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.3_SARS2_XBB.1.5

    Plasmid
    #196585
    Purpose
    expressing SARS-CoV-2 XBB.1.5 spike protein for pseudovirus production
    Depositor
    Insert
    Spike (XBB.1.5)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    G252V, F486P on the basis of XBB
    Promoter
    CMV
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. lenti-UCOE-dCas9-BFP-VP64

    Plasmid
    #196717
    Purpose
    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.
    Depositor
    Insert
    dCas9-TagBFP-VP64
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    D10A, N863A
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pRRlsinPGK_H2BGFP_WPRE

    Plasmid
    #91788
    Purpose
    Constitutive expression of H2BGFP fusion using PGK promoter in a lentiviral backbone.
    Insert
    Human histone H2B (H2BC11 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    PGK
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. PGAM5

    Plasmid
    #38976
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    PGAM5 (PGAM5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  6. pAAV-hSyn-DO-ChroME2f-ST-P2A-GCaMP8s

    Plasmid
    #227786
    Purpose
    Cre off - Cation channelrhodopsin ChroME2f fused to GCaMP8m and targeted to the neuronal soma and proximal dendrites
    Depositor
    Insert
    DO-ChroME2f-ST-P2A-GCaMP8s
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pAAV-hSyn-fDIO-mCherry-WPREpA

    Plasmid
    #177328
    Purpose
    Control vector for Flp-dependent DREADD constructs
    Depositor
    Insert
    mCherry
    Use
    AAV; Flp-frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human Synapsin-1
    Available since
    Dec. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pGL003

    Plasmid
    #215335
    Purpose
    amyE integration vector for IPTG-inducible expression in Bacillus subtilis (Pspank promoter)
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pSpank
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. USP2

    Plasmid
    #36894
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    USP2 (USP2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits
  10. pET-Impact-T4gp43(exo-)

    Plasmid
    #228202
    Purpose
    Expression in BL21(DE3) of 3' to 5' exonuclease-deficient T4 DNA polymerase D219A
    Depositor
    Insert
    43
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated aspartate 219 to alanine (D219A), elimina…
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  11. BirA(R118G)-HA destination vector

    Plasmid
    #53581
    Purpose
    To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)
    Depositor
    Type
    Empty backbone
    Use
    Tags
    BirA(R118G) and HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  12. pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro

    Plasmid
    #28289
    Purpose
    3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 20, 2011
    Availability
    Academic Institutions and Nonprofits only
  13. pCAGGs-Venus

    Plasmid
    #127346
    Purpose
    Expresses Venus constitutively via the CAGGS promoter in mammalian cells
    Depositor
    Insert
    Venus Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAGGS Promoter
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. pSB700-mouse-TTN-Cerulean

    Plasmid
    #128325
    Purpose
    SP-dCas9 compatible gRNA to be used as a positive control for activation experiments (mouse cells)
    Depositor
    Insert
    gRNA against mouse TTN for activation
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
  15. AAV-CAG-jGCaMP8m-WPRE

    Plasmid
    #179255
    Purpose
    AAV-mediated expression of GCaMP8m under the CAG promoter
    Depositor
    Has Service
    AAV Retrograde, AAV1, and AAV9
    Insert
    jGCaMP8m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Feb. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  16. pBS L30 mRuby3-Rab18

    Plasmid
    #166431
    Purpose
    Rab18 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Insert
    Rab18
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    L30 weak promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  17. pQCXIP-VEGFA121

    Plasmid
    #73017
    Purpose
    Retroviral expression of human VEGFA121
    Depositor
    Insert
    VEGFA (VEGFA Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  18. pAAV-tMCK-mem-TurboID

    Plasmid
    #149410
    Purpose
    Muscle-specific expression of secretory pathway membrane-localized TurboID, N-term HA tag, C-term V5 tag, in AAV expression plasmid. Amp selection
    Depositor
    Insert
    mem-TurboID
    Use
    AAV
    Tags
    HA and V5
    Expression
    Mutation
    Promoter
    tMCK
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. ORFtag_TetR-SD_Frame2

    Plasmid
    #220982
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame2
    Depositor
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  20. ORFtag_TetR-SD_Frame3

    Plasmid
    #220983
    Purpose
    ORFtag viral retroviral vector containing a constitutively active promoter and a TetR tag followed by a splice donor sequence in Frame3
    Depositor
    Insert
    TetR repressor
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. pET28-3'TwStrep

    Plasmid
    #208053
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag (with TAA) between HindII and XhoI site of pET28a (+), both HindIII and XhoI are retained. HindIII in-frame with Twin Strep tag.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Cbh and U6
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
  23. pET-MBP-NLS-Geo_st

    Plasmid
    #87703
    Purpose
    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS
    Depositor
    Insert
    GeoCas9
    Use
    Tags
    10xHis-MBP-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
  24. pcDNA3.1-hSyncytin-1(del480)

    Plasmid
    #182441
    Purpose
    hSyncytin-1, deletion 481-538
    Depositor
    Insert
    ERVW-1 (ERVW-1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deletion 481-538
    Promoter
    CMV
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
  25. LentiCROP-PuroR-P2A-GFP-U6

    Plasmid
    #200029
    Purpose
    Lentiviral CROP-seq vector backbone with PuroR-P2A-GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    PuroR-P2A-GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
  26. pET28-5'TwStrep

    Plasmid
    #208620
    Purpose
    Empty vector for prokaryotic expression, insert a Twin Strep tag between NdeI and BamHI site of pET28a (+), both site retained, T7 tag replaced, BamHI site in-frame with Twin Strep tag.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  27. PYCR1

    Plasmid
    #38842
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    PYCR1 (PYCR1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  28. pBS L30 mRuby3-Rab6b

    Plasmid
    #166427
    Purpose
    Rab5C fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Insert
    Rab6B
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    L30 weak promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  29. TTC5

    Plasmid
    #39064
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    TTC5 (TTC5 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  30. pBS-L1PA1CH_blast rescue

    Plasmid
    #69611
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts
    Depositor
    Insert
    L1PA1
    Use
    Tags
    blast rescue cassette
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
  31. pEGFP-N2-GRASP65

    Plasmid
    #137709
    Purpose
    Eukaryotic expression of rat GRASP65 full-length
    Depositor
    Insert
    GRASP65 (Gorasp1 Rat)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 2, 2020
    Availability
    Academic Institutions and Nonprofits only
  32. DFNB31-TwinStrepII

    Plasmid
    #83196
    Purpose
    Expression of tagged protein in mammalian cells
    Insert
    DFNB31 (Whrn Mouse)
    Use
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  33. pLIX-REST

    Plasmid
    #91896
    Purpose
    Doxycycline inducible expression of mouse REST
    Depositor
    Insert
    REST (Rest Mouse)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE promoter, Tet ON
    Available since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  34. pBS L30 mRuby3-Rab10

    Plasmid
    #166429
    Purpose
    Rab10 fused to mRuby3 fluorescent protein (ex558/em592) tag in N-ter under control of a weak L30 promoter.
    Depositor
    Insert
    Rab10
    Use
    Tags
    mRuby3
    Expression
    Mammalian
    Mutation
    Promoter
    L30 weak promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
  35. pCMV-Tag4-OMA1

    Plasmid
    #61719
    Purpose
    mammalian expression of human Oma1 with C-terminal FLAG tag
    Depositor
    Insert
    OMA1 (OMA1 Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
  36. Halo-Sec61-C-18

    Plasmid
    #123285
    Purpose
    Localization: Endoplasmic Reticulum
    Depositor
    Insert
    Sec61 (SEC61B Human)
    Use
    Tags
    HaloTag7
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 24, 2019
    Availability
    Academic Institutions and Nonprofits only
  37. PTGR1

    Plasmid
    #38874
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    PTGR1 (PTGR1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  38. p-dCas9-VP64-Hygro

    Plasmid
    #104405
    Purpose
    transient expression of dCas9-VP64 fusion protein
    Depositor
    Insert
    VP64
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
Showing: 3161 - 3200 of 8358 results