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pIDTsmart-RC2-delVP12-VP12-454TAG

Plasmid

#240061

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP1 and VP2

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP12
VP1-454-TAG
VP2-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-KO-delVP1-VP1-454TAG

Plasmid

#240062

Purpose

Express AAV2-KO (R585A,R588A) capsid with ncAA mutant at site 454 of VP1

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP1-R585,588A
VP1-454-TAG-R585,588A

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-H548A

Plasmid

#238319

Purpose

ScVip1-536-1107-H548A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-H548A

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF5x1-mRuby2-miRE.FF5-bGH

Plasmid

#235259

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5 design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF6x1-mRuby2-miRE.FF5-bGH

Plasmid

#235258

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF5 design 3

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 16, 2025

Availability

Academic Institutions and Nonprofits only

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pALN132

Plasmid

#239799

Purpose

Express mEGFP tagged mouse CENPT chimeric protein containing the rat CENPT histone fold domain

Depositor

Iain Cheeseman, Ally Nguyen

Article

Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

Insert

MmCENPT--Rn-Histone Fold Domain

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Promoter

Moloney Murine Leukemia Virus (MMLV) LTR promoter

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107

Plasmid

#238312

Purpose

ScVip1-536-1107 protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-K554A

Plasmid

#238329

Purpose

ScVip1-536-1107-K554A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-K554A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R547A

Plasmid

#238318

Purpose

ScVip1-536-1107-R547A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R547A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBB3-ScVip1-536-1107-R551A

Plasmid

#238320

Purpose

ScVip1-536-1107-R551A protein expression in insect cells

Depositor

Michael Hothorn

Article

Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

Insert

VIP1 (VIP1 Budding Yeast)

Tags

His10-Tag, Twin-Strep-tag, TEV

Expression

Insect

Mutation

ScVip1-536-1107-R551A

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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