Addgene

We narrowed to 14,720 results for: nans

Showing: 1601 - 1620 of 14720 results

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pCDNA-FRT-FAK-FERM0

Plasmid

#59125

Purpose

FAK fret control sensor without kinase domain with no linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-deltaPYD-GST

Plasmid

#51534

Purpose

Express GST-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

GST and T7

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pUHD Cdk2 DN HA

Plasmid

#27651

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Use

Tags

HA (YDVPDYA)

Expression

Mammalian

Mutation

D146N (Dominant Negative)

Promoter

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
pET21a-hAIM2-deltaPYD

Plasmid

#51522

Purpose

Express His6-tagged HIN domain of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

His6 and T7

Expression

Bacterial

Mutation

deletion of pyrin domain

Promoter

lac operator

Available since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-kinase10

Plasmid

#59123

Purpose

FAK fret control sensor without FERM domain with 10 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-PYD-GST

Plasmid

#51531

Purpose

Express GST-tagged PYD of hAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Tags

GST and T7

Expression

Bacterial

Mutation

deletion of amino acids 102-343

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pre_IV_CS6_4323_v1

Plasmid

#126859

Purpose

4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS6_4323_v1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5 KSR1 T274V

Plasmid

#25975

Depositor

Rob Lewis

Article

Brennan et al J Biol Chem. 2002 Feb 15. 277(7):5369-77

Insert

Kinase suppressor of Ras 1 (Ksr1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

changed threonine 274 to valine

Promoter

Available since

Aug. 24, 2010

Availability

Academic Institutions and Nonprofits only
Prp2-pRSETA

Plasmid

#61042

Purpose

Encodes the yeast Prp2 protein for overexpression and purification using a C-terminal hexahistidine tag.

Depositor

Nils Walter

Article

Krishnan et al Nat Struct Mol Biol. 2013 Dec;20(12):145

Insert

Prp2

Use

Tags

Histidine Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 27, 2015

Availability

Academic Institutions and Nonprofits only
pRSETb-rsGreen0.8

Plasmid

#78177

Purpose

Expresses rsGreen0.8 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.8

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-FERM10

Plasmid

#59126

Purpose

FAK fret control sensor without kinase domain with 10 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Use

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pmEmerald_mEmerald_I3

Plasmid

#231681

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with two tandem mEmeralds in mammalian cells. Assembles into nanocages tagged with 120 mEmerald FPs.

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

Insert

I3-01

Use

Tags

mEmerald

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pmEmerald_I3

Plasmid

#231680

Purpose

Expresses the I3-01 nanocage subunit N-terminally tagged with one mEmerald in mammalian cells. Assembles into nanocages tagged with 60 mEmerald FPs.

Depositor

Torsten Wittmann

Article

Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

Insert

I3-01

Use

Tags

mEmerald

Expression

Mammalian

Mutation

K129A

Promoter

CMV

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20-HA-TEADiv2

Plasmid

#228877

Purpose

Tet-inducible lentiviral vector for expression of optimized 2xHA-TEAD inhibitor (TEADiv2)

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Lentiviral

Tags

2xHA

Expression

Mutation

Promoter

Available since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET29_anti-SARS-CoV-2-NM1226

Plasmid

#209422

Purpose

Express anti-SARS-CoV-2-Nanobody NM1226 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1226

Use

Tags

Myc-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pET29_anti-SARS-CoV-2-NM1230

Plasmid

#209423

Purpose

Express anti-SARS-CoV-2-Nanobody NM1230 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-SARS-CoV-2-NM1230

Use

Tags

Myc-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR-HA-TEADiv2

Plasmid

#228875

Purpose

Gateway entry vector for optimized 2xHA-TEAD inhibitor (TEADiv2)

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Gateway entry vector

Tags

2xHA

Expression

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-EGFP-TEADiv2

Plasmid

#228874

Purpose

Gateway entry vector for optimized GFP-TEAD inhibitor (TEADiv2)

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Gateway entry vector

Tags

GFP

Expression

Mutation

Promoter

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAF201

Plasmid

#222376

Purpose

Recombinant Tasmanian devil IgG antibody (1F8) that binds to Tasmanian devil PDL1

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAF200

Plasmid

#222375

Purpose

Recombinant Tasmanian devil IgG antibody (3D5) that binds to 6xHis at the C-terminus of proteins

Depositor

Andrew S. Flies

Article

Slyp et al Methods Mol Biol. 2024;2826:231-249. doi

Insert

IGHG

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge