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DCT.VV.Orf164

Plasmid

#12852

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf164

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf270

Plasmid

#12958

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf270

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf159

Plasmid

#12847

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf159

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf95

Plasmid

#12783

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf95

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf71

Plasmid

#12759

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf71

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf187

Plasmid

#12875

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf187

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
DCT.VV.Orf74

Plasmid

#12762

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf74

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pRSF-G1(7CNW)RS

Plasmid

#222874

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Cyano-L-Tryptophan (7-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(5CNW)RS

Plasmid

#222872

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1_dCas9-XTEN-KRAB-p2A-mCherry

Plasmid

#222107

Purpose

Donor vector for CRISPRi integration at AAVS1 with an mCherry fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)

Use

AAV and CRISPR

Tags

HA and P2A-mCherry

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Available since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAVS1_dCas9-XTEN-KRAB-p2A-GFP

Plasmid

#222108

Purpose

Donor vector for CRISPRi integration at AAVS1 with a GFP fluorophore

Depositor

Jonathan Weissman

Article

She et al Cell. 2023 Jul 6;186(14):2977-2994.e23.

Insert

CRISPRi-kox1(KRAB) (cas9 S. pyogenes, Human)

Use

AAV and CRISPR

Tags

HA and P2A-GFP

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Available since

Oct. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM-U6-reRNA-mmSCN9A

Plasmid

#205636

Purpose

reRNA guide targeting Mus Musculus SCN9A

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

reRNA-mmSCN9A (Scn9a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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PGK-LCB3_v1.2-NotchGal4VP64-T2A-mCherry (pZYW047)

Plasmid

#194230

Purpose

Lentiviral expression vector expressing LCB3 SynNotch and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

PGK-LCB3-SynNotch-T2A-mCherry

Use

Lentiviral

Tags

Expression

Mutation

E45D mutation in LCB3

Promoter

PGK

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-LCB1_v1.3-PDGFR-T2A-mCherry (pZYW048)

Plasmid

#194231

Purpose

Lentiviral expression vector expressing LCB1 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a-LCB1-PDGFR TMD-T2A-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-LCB3_v1.2-PDGFR-T2A-mCherry (pZYW049)

Plasmid

#194232

Purpose

Lentiviral expression vector expressing LCB3 on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction marker

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv 2021.04.20.440678

Insert

EF1a-LCB3-PDGFR TMD-T2A-mCherry

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSUMO2_Ideonella-bGSDM-WELQ

Plasmid

#222098

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Ideonella species with a WELQut protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Ideonella-bGSDM-WELQ ORF

Use

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSUMO2_Vitiosangium-bGSDM-thrombin

Plasmid

#222099

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Vitiosangium species with a thrombin protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Vitiosangium-bGSDM-thrombin ORF

Use

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSUMO2_Nostoc-bGSDM-WELQ

Plasmid

#222100

Purpose

For expression of a mutant bacterial gasdermin (bGSDM) encoded by a Nostoc species with a WELQut protease cleavage site for controlled activation.

Depositor

Philip Kranzusch

Article

Johnson et al Nature. 2024 Apr;628(8008):657-663. doi:

Insert

Nostoc-bGSDM-WELQ ORF

Use

Tags

6xHis-SUMO2

Expression

Bacterial

Mutation

N-terminal methionine deleted and replaced by sin…

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAL4-DBD-NFAT5-WT

Plasmid

#215597

Purpose

For overexpression of Gal4-DBD NFAT5 WT

Depositor

Denes Hnisz

Article

Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

Insert

NFAT5 (NFAT5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
sgVRK2-puro

Plasmid

#199636

Purpose

sgRNA guide against VRK2

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge