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pFB-CG-LYCHOS WT-HA-GFP

Plasmid

#199687

Purpose

Expression of LYCHOS WT in insect cells

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

LYCHOS (GPR155 Human)

Use

Tags

10xHis, EGFP, HA, HRV 3C, and His

Expression

Insect

Mutation

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) ∆DEP-HA

Plasmid

#199683

Purpose

Transient expression of LYCHOS (GPR155) ∆DEP-HA

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

DEP domain deleted

Promoter

CMV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 LYCHOS (GPR155) Y551A-HA

Plasmid

#199676

Purpose

Transient expression of LYCHOS (GPR155) Y551A mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Y551 mutated to A

Promoter

CMV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ LED WT-FLAG

Plasmid

#199679

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED WT (GPR155 Human)

Use

Tags

6xHis and Flag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) P44A-HA

Plasmid

#199675

Purpose

Transient expression of LYCHOS (GPR155) P44A mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

P44 mutated to A

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) E48Q-HA

Plasmid

#199662

Purpose

Transient expression of LYCHOS (GPR155) E48Q mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

E48 mutated to Q

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) F43I-HA

Plasmid

#199674

Purpose

Transient expression of LYCHOS (GPR155) F43I mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

F43 mutated to I

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCPF101-pfQC-cyclase dead

Plasmid

#184878

Purpose

expresses soluble plasmodium falciparum QC protein with a histag, cyclase dead mutant

Depositor

Ferenc Scheeren

Article

Kolli et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

pfQC-cyclase dead

Use

Tags

His tag

Expression

Bacterial

Mutation

F77A & Q79A

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pCSmEn2n4ERT(#234)

Plasmid

#184082

Purpose

cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

6his-myc-chkEn2(n4)-ERT2

Use

Synthetic Biology

Tags

6his-Myc

Expression

Mammalian

Mutation

chkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…

Promoter

sCMV+SP6

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCSmEn2SRERT(#231)

Plasmid

#184080

Purpose

cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

6his-myc-chkEn2(SR)-ERT2

Use

Synthetic Biology

Tags

6his-Myc

Expression

Mammalian

Mutation

chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …

Promoter

sCMV+SP6

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22UbhaEng2aE4PCh(#271)

Plasmid

#184085

Purpose

cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

3HA-ZfEng2a-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

3xHA

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pT22UbhaEng2bE4PCh(#272)

Plasmid

#184086

Purpose

cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

3HA-ZfEng2b-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

3xHA

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22gCfpUbm5En2E4PCh(#251)

Plasmid

#184083

Purpose

cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-chkEn2-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22Ubm5Eng2bE4PCh(#284)

Plasmid

#184088

Purpose

cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-ZfEng2b-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22UbHEn2E4PCh(#262)

Plasmid

#184084

Purpose

cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

3HA-chkEn2-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

3xHA

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT22Ubm5Eng2aE4PCh(#283)

Plasmid

#184087

Purpose

cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

5myc-ZfEng2a-ERT2-P2A-mCherry

Use

Zebrafish transgenesis (blue eyes)

Tags

5xMyc

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSmEn25EERT(#232)

Plasmid

#184081

Purpose

cyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesis

Depositor

Sophie Vriz

Article

Rampon et al Development. 2015 May 15;142(10):1840-9.

Insert

6his-myc-chkEn2(5E)-ERT2

Use

Synthetic Biology

Tags

6his-Myc

Expression

Mammalian

Mutation

chkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …

Promoter

sCMV+SP6

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.25_S6

Plasmid

#173960

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 6 (S6) from SOX9 enhancer cluster EC1.25

Use

Luciferase

Tags

Expression

Mutation

None

Promoter

SV40 promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.35_S1

Plasmid

#173954

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region 1 (S1) from SOX9 enhancer cluster EC1.35

Use

Luciferase

Tags

Expression

Mutation

None

Promoter

SV40 promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-noSV40-humanEC1.35_S1-2

Plasmid

#173953

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer regions 1 and 2 (S1 and S2) from SOX9 enhancer cluster EC1.35

Use

Luciferase

Tags

Expression

Mutation

None

Promoter

SV40 promoter

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge