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CP2-TCC

Plasmid

#179679

Purpose

Complementary plasmid

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd2] T7 RNAP-TCC linker-degron

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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p5E-QUAS5x

Plasmid

#155113

Purpose

5' entry vector containing QUAS5x promoter element.

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUAS5x

Use

Gateway 5' entry vector

Tags

Expression

Mutation

Promoter

QUAS5x-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pJB01

Plasmid

#111957

Purpose

Expresses His10-RFP-Halo; construct generated for pull-down and localization experiments

Depositor

Matthew Good

Article

Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

Insert

RFP

Use

Tags

HaloTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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Hp MTAN

Plasmid

#69802

Purpose

Expression of H. pylori J99 methylthioadenosine nucleosidase

Depositor

Vern Schramm

Article

Wang et al Biochemistry. 2012 Sep 4;51(35):6892-4.

Insert

Methylthioadenosine nucleosidase

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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Spy0469

Plasmid

#159996

Purpose

Expresses Streptococcus pyogenes Spy0469 (24-389) in bacterial cells

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

Spy0469 (24-389)

Use

Tags

His6-tag and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4a G12V

Plasmid

#83169

Purpose

Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12V mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G12V

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-LssmOrange

Plasmid

#96937

Purpose

Retroviral plasmid containing LssmOrange for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Long Stokes Shift Monomeric Orange

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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ss-cfSGFP2

Plasmid

#37535

Depositor

Ikuo Wada

Article

Suzuki et al PLoS One. 2012;7(5):e37551. Epub 2012 Ma

Insert

ss-cfSGFP2 (cysteine-free SGFP2)

Use

Tags

signal sequence of α1-antitrypsin (M1-K34)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pJK240

Plasmid

#71127

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Use

Tags

GyrA intein and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_tdTomato

Plasmid

#62407

Purpose

MXS_chaining vector with tdTomato

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

tdTomato

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-mVenus

Plasmid

#67719

Purpose

Multisite gateway entry clone for adding C-terminal fusions of mVenus (no ATG)

Depositor

Rob Parton

Article

Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

Insert

mVenus

Use

Gateway multisite 3' entry clone

Tags

Expression

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pENTR4-FKBP12DD N-term C (w385-10)

Plasmid

#17416

Purpose

Gateway entry vector for Degron, N-term, frame C

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

FKBP12DD (L106P)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pSY45

Plasmid

#48355

Purpose

Targets TbMCM-BP for Cre-lox mediated knockout in T. brucei

Depositor

George Cross

Article

Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

Insert

loxP-SAS-HYG-Ty1-TK-loxP flanked by TbMCM-BP-targeting homologies

Use

Cre/Lox; Knockout in t. brucei

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

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M26I DJ-1 pET15b

Plasmid

#60677

Purpose

Recombinant protein expression of Park7

Depositor

Mark Wilson

Article

Lakshminarasimhan et al Biochemistry. 2008 Feb 5;47(5):1381-92.

Insert

M26I DJ-1 (PARK7 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

M26I

Promoter

T7

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
Hs.KRAS4a G12C

Plasmid

#83167

Purpose

Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G12C mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

G12C

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET28b-pchG WT

Plasmid

#87107

Purpose

expresses PchG WT, the thiazolinyl imine reductase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Meneely et al Biochemistry. 2016 Sep 27;55(38):5423-33

Insert

pchG WT

Use

Tags

his-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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MXS_TagBFP

Plasmid

#62400

Purpose

MXS_chaining vector with TagBFP

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

TagBFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_iRFP670

Plasmid

#62411

Purpose

MXS_chaining vector with iRFP670

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

iRFP670

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_mAzamiGreen

Plasmid

#62404

Purpose

MXS_chaining vector with mAzamiGreen

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

mAzamiGreen

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEN_hUmiRc2

Plasmid

#25747

Purpose

Entry vector for cloning miR30-based shRNA driven by human U6 promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only

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