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Addgene

We narrowed to 8,865 results for: BLI;

Showing: 4051 - 4100 of 8865 results
  1. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Insert
    MDH-2 (mdh-2 Nematode)
    Use
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pIND4-GFP-MmXYRS

    Plasmid
    #222745
    Purpose
    Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinker
    Depositor
    Inserts
    sfGFP
    MmXYRS
    Mm Pyl tRNA
    Use
    Tags
    6xHis
    Expression
    Mutation
    Contains the following substitutions: V346A, W348…
    Promoter
    rrnB
    Available since
    Sept. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. H2B-Electra2

    Plasmid
    #218929
    Purpose
    Electra2 fused to human H2B protein for nuclear localization
    Depositor
    Insert
    H2B-Electra2 (H2B Human, Entacmaea quadricolor, Synthetic)
    Use
    Synthetic Biology
    Tags
    Electra2 and H2B
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. LvPolyUb

    Plasmid
    #218982
    Purpose
    L.variegatus polyubiquitin-C promoter
    Depositor
    Insert
    L.variegatus polyubiquitinC promoter
    Use
    Sea urchin
    Tags
    Expression
    Mutation
    Promoter
    L.variegatus polyubiquitinC promoter
    Available since
    Aug. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pet28a+_Yjjg

    Plasmid
    #220228
    Purpose
    Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar
    Depositor
    Insert
    Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
    Use
    Tags
    Histag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 18, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. MUT_S1-H1,NR

    Plasmid
    #218395
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site
    Depositor
    Insert
    Modified CHO DHFR minigene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetracycline-responsive promoter
    Available since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
  8. 1167A

    Plasmid
    #218233
    Purpose
    HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene
    Depositor
    Insert
    putative metabolite transport protein
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    May 16, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pET28-Flag-RBM24

    Plasmid
    #208621
    Purpose
    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)
    Depositor
    Insert
    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    Use
    Tags
    6xHis and Flag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pET-29b(+)_notufA-EmEndoF2-CBD-His

    Plasmid
    #218197
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2
    Depositor
    Insert
    EndoF2
    Use
    Tags
    CBD-His
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-335
    Promoter
    T7
    Available since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  11. pAG426-GAL-Prepro-3x-Beta-Amyloid

    Plasmid
    #181730
    Purpose
    Galactose inducible expression of Prepro-3x-Beta-Amyloid
    Depositor
    Insert
    Prepro-3x-Beta-Amyloid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  12. pAG426-GAL-Kar2-Beta-Amyloid

    Plasmid
    #181707
    Purpose
    Galactose inducible expression of Kar2-Beta-Amyloid
    Depositor
    Insert
    Kar2-Beta-Amyloid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. E3_pENTR_L3-L2_NI_G1333-DddA11-N

    Plasmid
    #191609
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  14. E3_pENTR_L3-L2_NN_G1333-DddA11-N

    Plasmid
    #191610
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  15. E1_pENTR_L1-L4_HD_G1333-DddA11-C

    Plasmid
    #191599
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  16. E1_pENTR_L1-L4_NG_G1333-DddA11-C

    Plasmid
    #191600
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  17. E1_pENTR_L1-L4_NI_G1333-DddA11-C

    Plasmid
    #191601
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  18. E1_pENTR_L1-L4_NN_G1333-DddA11-C

    Plasmid
    #191602
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. E3_pENTR_L3-L2_HD_G1333-DddA11-N

    Plasmid
    #191607
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  20. E3_pENTR_L3-L2_NG_G1333-DddA11-N

    Plasmid
    #191608
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  21. pT7CFE1_polio_VP12A

    Plasmid
    #212988
    Purpose
    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Insert
    Poliovirus type I Mahoney VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Expression
    Mutation
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  22. pcDNA3-hFCER1G-VenusFlag

    Plasmid
    #211899
    Purpose
    Expresses human FCER1G in mammalian cells
    Depositor
    Insert
    FCER1G (FCER1G Human)
    Use
    Tags
    VenusFlag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  23. pNHA_OROV_NSs

    Plasmid
    #208934
    Purpose
    Expresses N-terminally HA-tagged Oropouche orthobunyavirus NSs protein from CMV promoter in mammalian cells
    Depositor
    Insert
    NSs
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Deleted 69 bp at 5' end (including ATG) and …
    Promoter
    CMV
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
  24. pPB-CAG-NPHP1

    Plasmid
    #209769
    Purpose
    Human NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Insert
    NPHP1 (NPHP1 Human, Synthetic)
    Use
    Piggybac transposon
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
  25. pCR8GW-HA-Wnk

    Plasmid
    #203967
    Purpose
    HA-Wnk basic construct
    Depositor
    Insert
    Wnk (most similar to RO variant) (Wnk Fly)
    Use
    Unspecified
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  26. pENTR3cDual-Fray

    Plasmid
    #203977
    Purpose
    Enrty clone for Gateway cloning
    Depositor
    Insert
    Frayed (fray Fly)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  27. PVALB mA_BCN14.1.1C9_LC_Mouse Ig Kappa

    Plasmid
    #182889
    Purpose
    Mammalian expression of PVALB mouse mAb LC gene
    Depositor
    Insert
    anti-PVALB (human) light chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  28. PVALB mAb_BCN14.1.1C9_LC_Rat Ig Kappa

    Plasmid
    #182891
    Purpose
    Mammalian expression of PVALB rat mAb LC gene
    Depositor
    Insert
    anti-PVALB (human) light chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  29. PVALB mAb_BCN14.1.1A2_HC_Mouse IgG2a

    Plasmid
    #182892
    Purpose
    Mammalian expression of PVALB mouse mAb HC gene
    Depositor
    Insert
    anti-PVALB (human) heavy chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  30. PVALB mAb_BCN14.1.1A2_LC_Mouse Ig Kappa

    Plasmid
    #182893
    Purpose
    Mammalian expression of PVALB mouse mAb LC gene
    Depositor
    Insert
    anti-PVALB (human) light chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
  31. pcDNA3.1-Bid-GFP

    Plasmid
    #191938
    Purpose
    Expression of BID-GFP chimera
    Depositor
    Insert
    Bid-GFP (Bid Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
  32. pLIB_BSSHI_6xHisTEV-WIPI2d

    Plasmid
    #190863
    Purpose
    Plasmid for the expression of purification of WIPI2d: SMC Internal reference: SMC1216
    Depositor
    Insert
    WIPI2 (WIPI2 Human)
    Use
    Tags
    Expression
    Bacterial and Insect
    Mutation
    SNP C to G at position 4562 (Val)
    Promoter
    Available since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  33. pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Plasmid
    #170788
    Purpose
    Expresses pET-15b(+) Mortierella verticillate (delta prenylation motif)
    Depositor
    Insert
    Mortierella verticillate PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
  34. pCW57.1-GFP_mmu-miR-16-5p_sponge

    Plasmid
    #132624
    Purpose
    Expresses a TurboGFP transcript with microRNA sponge for mmu-miR-16-5p as 3'UTR
    Depositor
    Insert
    stop codon, mmu-miR-16-5p sponge sequence and polyA signal
    Use
    Lentiviral; Doxycycline inducible
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    Aug. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
  35. p159-RV-syn-dio-mScarlet-T2A

    Plasmid
    #185700
    Purpose
    Retroviral vector expression cre-dependent membrane tagged mScarlet-T2A driven by the rat Synapsin promoter (with XhoI and PacI at 3'end of T2A to clone a gene of interest)
    Depositor
    Insert
    mScarlet
    Use
    Retroviral
    Tags
    mScarlet palmitoylation with T2A on 3'-end
    Expression
    Mutation
    Promoter
    Synapsin
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
  36. pGEMHE-TNKS-2-M1054V

    Plasmid
    #138538
    Purpose
    For making TNKS-2-M1054V RNA
    Depositor
    Insert
    Tankyrase 2 (TNKS2 Human)
    Use
    In vitro transcription
    Tags
    FLAG
    Expression
    Mutation
    Changed methionine 1054 to valine
    Promoter
    T7
    Available since
    June 22, 2022
    Availability
    Academic Institutions and Nonprofits only
  37. pAAV-CaMKIIa-hM4D(Gi)-mCherry-WPRE-pA(Short)

    Plasmid
    #183704
    Purpose
    AAV to express hM4D(Gi)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit
    Depositor
    Insert
    hM4D(Gi)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    CaMKIIa
    Available since
    May 4, 2022
    Availability
    Academic Institutions and Nonprofits only
  38. pAAV-CaMKIIa-hM3D(Gq)-mCherry-WPRE-pA(Short)

    Plasmid
    #183705
    Purpose
    AAV to express hM3D(Gq)-mCherry fusion protein. Viral genome 3' UTR modified to bring the viral genome below the optimal AAV packaging limit
    Depositor
    Insert
    hM3D(Gq)
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    CaMKIIa
    Available since
    May 3, 2022
    Availability
    Academic Institutions and Nonprofits only
  39. loxP-T2A-Myr-Casp8-ERT2-rox

    Plasmid
    #182362
    Purpose
    Shuttle vector for T2A-Myr-Casp8-ERT2
    Depositor
    Insert
    loxP-T2A-Myr-Casp8-ERT2-rox
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  40. loxP-T2A-Myr-DeadCasp8-ERT2-rox

    Plasmid
    #182361
    Purpose
    Shuttle vector for T2A-Myr-DeadCasp8-ERT2
    Depositor
    Insert
    loxP-T2A-Myr-DeadCasp8-ERT2-rox
    Use
    Cre/Lox and Mouse Targeting; Dre / rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  41. pMSP1D12W/2Y

    Plasmid
    #124275
    Purpose
    Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
    Depositor
    Insert
    Membrane scaffold protein
    Use
    Tags
    7xHis-tag
    Expression
    Bacterial
    Mutation
    Two tryptophans changed to two tyrosines
    Promoter
    T7
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  42. pCDZ2FE_Rev3_1873-2025

    Plasmid
    #105672
    Purpose
    Expresses Flag-tagged fragment (amino acids 1873-2025) of human Rev3 protein
    Depositor
    Insert
    Rev3L (REV3L Human)
    Use
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  43. pcDNA3.1-EOLA1/CXORF40A

    Plasmid
    #180776
    Purpose
    Expresses EOLA1/CXORF40A in mammalian cells
    Depositor
    Insert
    EOLA1 (EOLA1 Human)
    Use
    Tags
    SBP-Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
  44. SDCBPA-c020

    Plasmid
    #175484
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, G111-V288.
    Depositor
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  45. STX4AA-c005

    Plasmid
    #175487
    Purpose
    Protein expression in bacterial cells. Coiled-coil domains, M1-L275.
    Depositor
    Insert
    STX4 (RUNX1T1 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
  46. CAG-loxP-3xpA-loxP-sfGFP+HsbG syntron

    Plasmid
    #172477
    Purpose
    sfGFP with a synthetic intron derived from the human beta-globin gene
    Depositor
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  47. CAG-loxP-3xpA-loxP-sfGFP+pCI syntron

    Plasmid
    #172478
    Purpose
    sfGFP with a synthetic intron from the pCI expression vector (Promega)
    Depositor
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  48. CAG-loxP-3xpA-loxP-sfGFP+MmIghe syntron

    Plasmid
    #172475
    Purpose
    sfGFP with a synthetic intron derived from the mouse Ighe gene
    Depositor
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  49. CAG-loxP-3xpA-loxP-sfGFP+RbG syntron

    Plasmid
    #172476
    Purpose
    sfGFP with a synthetic intron derived from the rabbit beta-globin gene
    Depositor
    Insert
    sfGFP
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
Showing: 4051 - 4100 of 8865 results