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pJRoC30-LacAnc100

Plasmid

#234653

Purpose

Expresses LacAnc100 variant in yeast

Depositor

Miguel Alcalde Galeote, Alice Ting

Article

Lee et al Nat Chem Biol. 2025 Aug 1. doi: 10.1038/

Insert

LacAnc100

Tags

HRV 3C protease site-GSG linker-8xHis tag

Expression

Yeast

Promoter

Gal1p

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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FUS LC 8Y->S

Plasmid

#226606

Purpose

8 tyrosines to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 8Y->S (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

8 tyrosines to serine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-Trim21-OLLAS

Plasmid

#237438

Purpose

Expresses mouse Trim21 ubiquitin ligase tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

Depositor

Michael Lampson

Article

Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

Insert

Trim21 (Trim21 Mouse)

Tags

OLLAS

Expression

Mammalian

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)

Plasmid

#238167

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

GFP

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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XZ390 (IVT) CMV-TO-T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3

Plasmid

#233425

Purpose

IVT template for LIDAR reporter mRNA; mCherry as marker, rtetR-NZF as output

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

T7-mCherry-SGc(stem-1(3xMS2))cSG-rTetR-NZF-NLS-hybridUTR3

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40

Plasmid

#233368

Purpose

hSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_on-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40

Plasmid

#233367

Purpose

hSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA_T/A-mEGFP

Use

AAV

Tags

NES and mEGFP

Promoter

hSyn1

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_off-mEGFP

Plasmid

#233356

Purpose

CMV driven expression of the inactive kinase activity recorder negative control Kinprola_off in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_off-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_PKA_T/A-mEGFP

Plasmid

#233354

Purpose

CMV driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_PKA_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Kinprola_JNK_T/A-mEGFP

Plasmid

#233360

Purpose

CMV driven expression of the inactive JNK activity recorder negative control with T/A mutation Kinprola_JNK_T/A in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

Insert

NES-Kinprola_JNK_T/A-mEGFP

Tags

NES and mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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