We narrowed to 23,752 results for: Spr

1. CENPJ C8.1 gRNA

   Plasmid

   #90619

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CENPJ

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CENPJ (Guide Designation C8.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CENPE C4.1 gRNA

   Plasmid

   #90615

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CENPE

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CENPE (Guide Designation C4.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CENPE C3.1 gRNA

   Plasmid

   #90614

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CENPE

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CENPE (Guide Designation C3.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CENPB C2.1 gRNA

   Plasmid

   #90613

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CENPB

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CENPB (Guide Designation C2.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CDT1 B9.1 gRNA

   Plasmid

   #90608

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDT1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CDT1 (Guide Designation B9.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CDK1 B8.1 gRNA

   Plasmid

   #90605

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CDK1 (Guide Designation B8.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CDK1 B7.1 gRNA

   Plasmid

   #90604

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CDK1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CDK1 (Guide Designation B7.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BUB3 A9.1 gRNA

   Plasmid

   #90556

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human BUB3

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   BUB3 (Guide Designation A9.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CASC5 A11.1 gRNA

   Plasmid

   #90564

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CASC5

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   CASC5 (Guide Designation A11.1)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CASC5 A12.1 gRNA

    Plasmid

    #90565

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CASC5

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CASC5 (Guide Designation A12.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CENPA B12.1 gRNA

    Plasmid

    #90611

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CENPA

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CENPA (Guide Designation B12.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. BUB1B A7.1 gRNA

    Plasmid

    #90554

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BUB1B

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BUB1B (Guide Designation A7.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. BUB1B A8.1 gRNA

    Plasmid

    #90555

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BUB1B

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BUB1B (Guide Designation A8.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AURKB A4.1 gRNA

    Plasmid

    #90545

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human AURKB

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    AURKB (Guide Designation A4.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. BUB1 A5.1 gRNA

    Plasmid

    #90552

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BUB1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BUB1 (Guide Designation A5.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. BUB1 A6.1 gRNA

    Plasmid

    #90553

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human BUB1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    BUB1 (Guide Designation A6.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CCNB1 B4.1 gRNA

    Plasmid

    #90577

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CCNB1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CCNB1 (Guide Designation B4.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CCNB1 B3.1 gRNA

    Plasmid

    #90576

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CCNB1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CCNB1 (Guide Designation B3.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. DBF4 C9.1 gRNA

    Plasmid

    #90646

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DBF4

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DBF4 (Guide Designation C9.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. DSN1 C11.1 gRNA

    Plasmid

    #90656

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DSN1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DSN1 (Guide Designation C11.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. DSN1 C12.1 gRNA

    Plasmid

    #90657

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DSN1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    DSN1 (Guide Designation C12.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CENPF C6.1 gRNA

    Plasmid

    #90617

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human CENPF

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    CENPF (Guide Designation C6.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ESPL1 D7.1 gRNA

    Plasmid

    #90682

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ESPL1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    ESPL1 (Guide Designation D7.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GINS1 D11.1 gRNA

    Plasmid

    #90694

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human GINS1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    GINS1 (Guide Designation D11.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pY036_ATP1A1_G3

    Plasmid

    #86617

    Purpose

    Expresses the ATP1A1 G3 crRNA in combination with AsCpf1-3xHA to target ATP1A1 exon 4. U6-crRNA(ATP1A1 G3)-CBh-AsCpf1

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

    Insert

    ATP1A1 G3 crRNA + AsCpf1-3xHA
    

    Use

    CRISPR; Co-selection via nhej using ouabain

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PX458_MIER3_2

    Plasmid

    #86324

    Purpose

    Encodes gRNA for 3' target of human MIER3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against MIER3 (MIER3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PX458_KDM3A_1

    Plasmid

    #86321

    Purpose

    Encodes gRNA for 3' target of human KDM3A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KDM3A (KDM3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

28. PX458_HMG20A_2

    Plasmid

    #86319

    Purpose

    Encodes gRNA for 3' target of human HMG20A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against HMG20A (HMG20A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. PX458_KLF11_1

    Plasmid

    #86314

    Purpose

    Encodes gRNA for 3' target of human KLF11

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KLF11 (KLF11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PX458_MIER3_1

    Plasmid

    #86323

    Purpose

    Encodes gRNA for 3' target of human MIER3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against MIER3 (MIER3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. PX458_KDM3A_2

    Plasmid

    #86322

    Purpose

    Encodes gRNA for 3' target of human KDM3A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KDM3A (KDM3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. PX458_HMG20A_1

    Plasmid

    #86318

    Purpose

    Encodes gRNA for 3' target of human HMG20A

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against HMG20A (HMG20A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PX458_KLF11_2

    Plasmid

    #86315

    Purpose

    Encodes gRNA for 3' target of human KLF11

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KLF11 (KLF11 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PX458_KAT7_1

    Plasmid

    #86309

    Purpose

    Encodes gRNA for 3' target of human KAT7

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against KAT7 (KAT7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pT7-gRNA:Asip

    Plasmid

    #60968

    Purpose

    Expression vector of rat Asip guide RNA

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

    Insert

    Agouti signaling protein gRNA (Asip Rat)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

36. pT7-gRNA:kit-1

    Plasmid

    #60969

    Purpose

    Expression vector of rat Kit-1 guide RNA

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

    Insert

    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

37. pT7-gRNA:kit-2-1

    Plasmid

    #60970

    Purpose

    Expression vector of rat Kit-2-1 guide RNA

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

    Insert

    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pT7-gRNA:kit-2-2

    Plasmid

    #60971

    Purpose

    Expression vector of rat Kit-2-2 guide RNA

    Depositor

    Tomoji Mashimo

    Article

    Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

    Insert

    v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog gRNA (Kit Rat)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

39. UAS-u(XS)Cas9

    Plasmid

    #127382

    Purpose

    Expresses Cas9 at very high level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

    Plasmid

    #104563

    Purpose

    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)

    Depositor

    Keith Joung

    Article

    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

    Insert

    dLbCpf1 (D832A)
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Mutation

    D832A

    Promoter

    CAG

    Available since

    Jan. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pHR-SFFV-dCas9-BFP-KRAB

    Plasmid

    #46911

    Purpose

    Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS, tagBFP and a KRAB domain

    Depositor

    Jonathan Weissman, Stanley Qi

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-BFP-KRAB fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2xNLS, BFP, HA, and KRAB domain

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid

    #139987

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

    Plasmid

    #163024

    Purpose

    Expression of CRISPRi with U6-gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 polyA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    hEF-1a

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)

    Plasmid

    #204619

    Purpose

    Expression vector of Cascade and Cas3. Puromycin selectable.

    Depositor

    Akitsu Hotta

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    Cas7, Cas5, Cas8, Cas11, Cas6, and Cas3
    

    Use

    CRISPR

    Tags

    Each protein has C- and N-terminal SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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46. PRKAG3 gRNA (BRDN0001145578)

    Plasmid

    #77295

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. PRKAG3 gRNA (BRDN0001147132)

    Plasmid

    #77296

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. PRKAG3 gRNA (BRDN0001146704)

    Plasmid

    #77294

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAG3

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAG3 (PRKAG3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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50. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102886

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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