We narrowed to 13,711 results for: cas9

1. eSpCas9(1.1)-T2A-Puro

   Plasmid

   #101039

   Purpose

   Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

   Depositor

   Andrea Németh

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3xFLAG and T2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCas9/VRQR-sgRNA (BbsI)

   Plasmid

   #129725

   Purpose

   Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MO91-dCas9-AID(P182X)

   Plasmid

   #83260

   Purpose

   Expresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.

   Depositor

   Xing Chang

   Article

   Ma et al Nat Methods. 2016 Dec;13(12):1029-1035.

   Insert

   dCas9-AID P182X
   

   Use

   Retroviral

   Tags

   3xFLAG-NLS and FLAG

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9; P182X in AID

   Promoter

   MSCV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

4. px551-mSncg-0.27kb-HA-SpCas9

   Plasmid

   #153208

   Purpose

   Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

   Depositor

   Yang Hu

   Article

   Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

   Insert

   Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Truncated mSncg (0.27kb)

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Sth1Cas9n cytosine base editor

   Plasmid

   #194209

   Purpose

   Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

   Depositor

   Chase Beisel

   Article

   Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

   Insert

   Sth1Cas9-D9A nickase
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Changed Aspartate 9 to Alanine.

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-TRE-DIO-Cas9

   Plasmid

   #166601

   Purpose

   Encodes Cre-dependent Cas9 under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   Cas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-FLEX-SaCas9-U6-sgSlc18a2

   Plasmid

   #124849

   Purpose

   Mutagenesis of Slc18a2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Slc18a2 (Slc18a2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

   Plasmid

   #217662

   Purpose

   expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

   Plasmid

   #148859

   Purpose

   Mammalian Expression of HsXRN1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsXRN1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHAGE-EFS-dCas9-GFP

    Plasmid

    #64104

    Purpose

    Expression of Sp dCas9-GFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-dCas9-T2A-GFP

    Plasmid

    #193781

    Purpose

    Tricistronic expression of dCas9-EGFP-BSD

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    dead Cas9
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with existing Cas9 promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

    Plasmid

    #124862

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PET-B-evoSpCas9-NLS-6xHis

    Plasmid

    #207390

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p221z-CAS9p-TagRFP-t35s

    Plasmid

    #118386

    Purpose

    Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    CAS9p-TagRFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAC152-dual-dCas9VP64-sgExpression

    Plasmid

    #48238

    Purpose

    Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    HA Tag, HA-Tag, and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A

    Promoter

    Available since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHAGE-TO-dCas9-P2A-HSA

    Plasmid

    #121936

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    dCas9-P2A-HSA
    

    Use

    CRISPR and Lentiviral

    Tags

    HSA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-Dual-iCas9-EFS

    Plasmid

    #167501

    Purpose

    Expression of dual inducible Cas9 with EFS promoter

    Depositor

    Jake Liang

    Article

    Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

    Insert

    Cas9
    

    Use

    CRISPR, Lentiviral, and Luciferase

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. hAAVS1-gs4_EF1a-Cas9-2A-GFP

    Plasmid

    #118414

    Purpose

    CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    gRNA AAVS1 (AAVS1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV.minCMVe-SCP1-SaCas9-W3SynpA

    Plasmid

    #231370

    Purpose

    SCP1 promoter-driven SaCas9 with truncated CMV enhancer and short synthetic terminator sequence.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    SaCas9
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge