We narrowed to 13,947 results for: cas9

1. pAAV2ss-U6-sgHTT1-7sk-sgCas9

   Plasmid

   #190900

   Purpose

   AAV-KamiCas9 vector expressing sgGFP and human HTT sgRNAs

   Depositor

   Nicole Déglon

   Article

   Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

   Insert

   sgRNA (HTT spCas9, Human)
   

   Use

   AAV and CRISPR

   Promoter

   U6 and 7sk

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC154-dual-dCas9VP160-sgExpression

   Plasmid

   #48240

   Purpose

   Dual expression construct expressing both dCas9VP160 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA-Tag, HA-tag, and VP160

   Expression

   Mammalian

   Mutation

   D10A H840A

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS2699-CMV-Nme2Cas9-E932R

   Plasmid

   #228720

   Purpose

   Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2Cas9-E932R nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS2696-CMV-Nme2SmuCas9-D56R

   Plasmid

   #228717

   Purpose

   Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2SmuCas9-D56R nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEJS2697-CMV-Nme2SmuCas9-E932R

   Plasmid

   #228718

   Purpose

   Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2SmuCas9-E932R nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS2698-CMV-Nme2Cas9-D56R

   Plasmid

   #228719

   Purpose

   Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Nme2Cas9-D56R nuclease
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lenti-Dual-iCas9-CMV

   Plasmid

   #167500

   Purpose

   Expression of dual inducible Cas9 with CMV promoter

   Depositor

   Jake Liang

   Article

   Park et al Mol Ther Nucleic Acids. 2022 Apr 27;28:6

   Insert

   Cas9
   

   Use

   CRISPR, Lentiviral, and Luciferase

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-FLEX-SaCas9-U6-sgGria1

   Plasmid

   #124853

   Purpose

   Mutagenesis of Gria1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria1 (Gria1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-eSpCas9-NLS-6xHis

   Plasmid

   #207379

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   eSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   K848A, K1003A, R1060A

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX393 Gluconacetobacter diazotrophicus Cas9

    Plasmid

    #68330

    Purpose

    Gluconacetobacter diazotrophicus Cas9

    Depositor

    Feng Zhang

    Insert

    GdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. Dual-CRISPR-Cas9-U6-H1 (pBP48)

    Plasmid

    #218932

    Purpose

    The backbone dual-CRISPR plasmid containing Cas9 that is used for making dual-CRISPR plasmids.

    Depositor

    Baoxu Pang

    Article

    Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    2A-Puro and 3XHA

    Expression

    Mammalian

    Available Since

    July 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE EF1α dCas9-VP64

    Plasmid

    #50918

    Purpose

    Constitutive dCas9-VP64 lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and VP64

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    EF1alpha

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX383 Lactobacillus johnsonii Cas9

    Plasmid

    #68320

    Purpose

    Lactobacillus johnsonii Cas9

    Depositor

    Feng Zhang

    Insert

    LjCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEX-SaCas9-U6-sgSlc17a7

    Plasmid

    #124846

    Purpose

    Mutagenesis of Slc17a7

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a7 (Slc17a7 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX386 Lactobacillus farciminis Cas9

    Plasmid

    #68323

    Purpose

    Lactobacillus farciminis Cas9

    Depositor

    Feng Zhang

    Insert

    LfCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. U6-sgH11-eCas9-T2A-BlastR

    Plasmid

    #172492

    Purpose

    For CRISPR-assisted HDR, Hipp11 locus

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Insert

    , BlastR and sgRNA targeting the Hipp11 (H11) safe harbor locus
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. HiFi-nCas9-NG_P2A-mCherry

    Plasmid

    #219936

    Purpose

    Mammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systems

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    Sp-HiFi-nCas9(D10A)-NG_P2A-mCherry
    

    Use

    CRISPR

    Tags

    SV40 bpNLS and SV40 bpNLS-P2A-mCherry

    Expression

    Mammalian

    Mutation

    HiFi-nCas9(D10A/R691A)

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAVi U6_gRNA CMV_SadCas9-KRAB

    Plasmid

    #214609

    Purpose

    All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

    Depositor

    Ralf Gilsbach

    Article

    Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

    Inserts

    gRNA
    SadCas9
    

    Use

    AAV

    Tags

    NP NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, N580A

    Promoter

    CMV and U6

    Available Since

    March 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-SpCas9-P2A-puro

    Plasmid

    #214358

    Purpose

    Expresses SpCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 and puromycin resistance genes
    

    Expression

    Mammalian

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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