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We narrowed to 5,049 results for: golden gate

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Showing: 1661 - 1680 of 5049 results

  1. pHEE-R-bar9si

    Plasmid
    #222834
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, EC1 promoter, bialaphos and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222834-plasmid-map-sequence-id-446485

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  2. pHEE-R-hyg5A9si

    Plasmid
    #222835
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, hygromycin and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222835-plasmid-map-sequence-id-446486

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  3. pAIP4

    Plasmid
    #233686
    Purpose
    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.
    Depositor
    Christopher Bahl
    Insert
    Smt3 (SMT3 )
    Tags
    HisTag
    Promoter
    T7
    Available Since
    April 7, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    233686-plasmid-map-sequence-id-468017

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  5. pHEE-R-bar5A9si

    Plasmid
    #222837
    Purpose
    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, bialaphos and red fluorescent seed selection)
    Depositor
    Hidenori Takeuchi
    Type
    Empty backbone
    Use
    CRISPR
    Available Since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222837-plasmid-map-sequence-id-446488

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  6. pAIP4_4WDC

    Plasmid
    #234153
    Purpose
    Heterologous protein expression of DELTA-actitoxin-Afr1a (DELTA-AITX-Afr1a) (Fragaceatoxin C) (fraC) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4WDC
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234153-plasmid-map-sequence-id-464119

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  7. pUC-GW-Kan-L0-TaSEP1B6(int)

    Plasmid
    #242006
    Purpose
    L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum SEPALLATA1-6 (SEP1-6) B-subgenome homoeolog (TraesCS5B02G396700)
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242006-plasmid-map-sequence-id-504502

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  8. pRDB_031

    Plasmid
    #245331
    Purpose
    Cas12a CRISPRko all-in-one positive control; targets CD47, CD63
    Depositor
    John Doench, David Root
    Insert
    Cas12a, sgRNA CD47, CD63 (CD47 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245331-plasmid-map-sequence-id-488051

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  9. pRDB_245

    Plasmid
    #245332
    Purpose
    Cas12a CRISPRko positive control guide; targets CD46
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD46 (CD46 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245332-plasmid-map-sequence-id-488043

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  10. pRDA_837

    Plasmid
    #245328
    Purpose
    Cas9 CRISPRko positive control guide; targets CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245328-plasmid-map-sequence-id-488040

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  11. pRDB_247

    Plasmid
    #245334
    Purpose
    Cas12a CRISPRko positive control guide; targets CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245334-plasmid-map-sequence-id-488032

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  12. pMV_AA097

    Plasmid
    #245319
    Purpose
    Cas12a CRISPRko positive control; targets CD46, CD47, CD55
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD46, CD47, CD55 (CD46 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245319-plasmid-map-sequence-id-488024

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  13. pAIP4_6WEY

    Plasmid
    #234234
    Purpose
    Heterologous protein expression of replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6WEY (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234234-plasmid-map-sequence-id-464198

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  14. pAIP4_3D1B

    Plasmid
    #234230
    Purpose
    Heterologous protein expression of RNA-induced transcriptional silencing complex protein tas3 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3D1B (tas3 Fission Yeast)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234230-plasmid-map-sequence-id-464195

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  15. pAIP4_5HIH

    Plasmid
    #234205
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5HIH (orf1ab Middle East respiratory syndrome-related coronavirus)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234205-plasmid-map-sequence-id-464170

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  16. pAIP4_5EIP

    Plasmid
    #234188
    Purpose
    Heterologous protein expression of RNA binding exosome specificity factor Mmi1 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5EIP (mmi1 Fission Yeast)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234188-plasmid-map-sequence-id-464153

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  17. pAIP4_3ONH

    Plasmid
    #234179
    Purpose
    Heterologous protein expression of ubiquitin-activating enzyme E1-like in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3ONH (UBA2 Budding Yeast)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234179-plasmid-map-sequence-id-464144

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  18. pAIP4_1HKA

    Plasmid
    #234169
    Purpose
    Heterologous protein expression of 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1HKA (folK Escherichia coli)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234169-plasmid-map-sequence-id-464135

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  19. pAIP4_6BIO

    Plasmid
    #234152
    Purpose
    Heterologous protein expression of clan CA, family C40, NlpC/P60 superfamily cysteine peptidase in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6BIO (TVAG_298080 Trichomonas vaginalis)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234152-plasmid-map-sequence-id-464118

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  20. pAIP4_2H2Z

    Plasmid
    #234147
    Purpose
    Heterologous protein expression of replicase polyprotein 1a (pp1a) (ORF1a polyprotein) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2H2Z (ORF1ab Severe acute respiratory syndrome-related coronavirus)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234147-plasmid-map-sequence-id-464113

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Showing: 1661 - 1680 of 5049 results