-
Plasmid#38775DepositorInsertActin promoter and intron
UsePlant transformationTagsnos 3' terminator and sGFP (S65T)ExpressionMutationPromoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFH33
Plasmid#125885PurposeLevel0 Wheat TaU3 Pol III promoter with transcription Start-ADepositorInsertLevel0 Wheat TaU3 Pol III promoter with transcription Start-A
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-mCh-GFPi
Plasmid#21905DepositorInsertGFPi
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2009AvailabilityAcademic Institutions and Nonprofits only -
pICSL30003
Plasmid#50304DepositorInsertCDS, no stop codon, mCherry variant of RFP (Discosoma sp.)
UseTagsExpressionPlantMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPSdown
Plasmid#149417PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the 3'-UTR of the GFP reporter gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSup2
Plasmid#149418PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted about 1.4 kb upstream of the minimal promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL80004
Plasmid#50325DepositorInsertCDS, plant codon-optimsed variant of turbo RFP (Entacmaea quadricolor)
UsePlant expressionTagsExpressionMutationBsaI/BbsI sites removed by point-mutationPromoterAvailable sinceFeb. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mNeonGreen(2x)
Plasmid#197919PurposeHomo-dimer expression vector with plasma membrane localization signalDepositorInsertglycosylphosphatidylinositol-mNeonGreen-NeonGreen
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-mNeonGreen
Plasmid#197918PurposeMonomer expression vector with plasma membrane localization signalDepositorInsertglycosylphosphatidylinositol-mNeonGreen
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPSdown2
Plasmid#149419PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted downstream of the GFP reporter gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJOG130
Plasmid#105336PurposeGateway entry clone generationDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXf20pemIK
Plasmid#126577PurposeVector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stabilityDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH69
Plasmid#128189PurposeLevel1 ZmUBIp::ABE7.10 base changer (human codon optimized):NOStDepositorInsertZmUBIp::ABE7.10 base changer (human codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK220
Plasmid#71123PurposeExpression of E. coli pantotheinate kinase (H6PanK, R175H mutant) in E. coliDepositorInsertpantothenate kinase
UseTagsH6ExpressionBacterialMutationArg175 is mutated to a HisPromoterT7Available sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLSLGUS
Plasmid#51517PurposepLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmidDepositorInsertsGUS
3kb filler sequence
UsePlant t-dna plasmidTagsNLS and flagExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFH37
Plasmid#126011PurposeLevel0 Rice OsU6-2 Pol III promoter with transcription Start-GDepositorInsertLevel0 Rice OsU6-2 Pol III promoter with transcription Start-G
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
p35SZ
Plasmid#51513PurposepMDC32 with Zif268:FokI followed by approximately 3kb of filler sequenceDepositorInsertsZif268:FokI
3kb filler sequence
UsePlant expression t-dnaTagsNLSExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-Gamillus(2x)
Plasmid#197921PurposeHomo-dimer expression vector with plasma membrane localization signalDepositorInsertglycosylphosphatidylinositol-Gamillus-Gamillus
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
GPI-Gamillus(2x)
Plasmid#197915PurposeHomo-dimer expression vector with plasma membrane localization signalDepositorInsertglycosylphosphatidylinositol-Gamillus-Gamillus
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
myr-palm-Gamillus
Plasmid#197920PurposeMonomer expression vector with plasma membrane localization signalDepositorInsertglycosylphosphatidylinositol-Gamillus
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceApril 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397C_ABE
Plasmid#189643Purpose16S rRNA Right_G1397C_ABEDepositorInsertTALE and DddAtox and TadA 8e
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AatII-pRPS5A-PTP-3Xflag-16S rRNA Right_G1397N
Plasmid#189644Purpose16S rRNA Right_G1397NDepositorInsertTALE and DddAtox
UseTALENTagsExpressionPlantMutationPromoterRPS5AAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
AatII-pRPS5A-PTP-3Xflag- psa A Right _G1397C_ABE
Plasmid#189648Purposepsa A Right _G1397C_ABEDepositorInsertTALE and DddAtox and TadA 8e
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRPS5A-PTP-3Xflag-rbc L 294 Left_G1397 C-ABE
Plasmid#189650Purposerbc L 294 Left_G1397 C-ABEDepositorInsertTALE and DddAtox and TadA 8e
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH39
Plasmid#126013PurposeLevel0 Rice OsU3 Pol III promoter with transcription Start-ADepositorInsertLevel0 Rice OsU3 Pol III promoter with transcription Start-A
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXf20pemIK-GW
Plasmid#126576PurposeVector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability, Gateway cloning compatibleDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJOG131
Plasmid#105337PurposeGateway entry clone generationDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ142-ZmUbi-tRNA
Plasmid#158578PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB402-er-mCherry
Plasmid#186288PurposeBinary vector for the expression of er-mCherry (SP-mCherry-HDEL) in plants (for Agrobacterium-mediated genetic transformation and visualization of endoplasmic reticulum)DepositorInserter-mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAXY0001
Plasmid#179834Purposebinary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoterDepositorInserteYGFPuv
UseBinary vector for plant transformationTagsExpressionPlantMutationPromoter35SAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLSLGFP.R
Plasmid#51501PurposeSingle component LSL vector with GFP. Rep is expressed from the complementary sense LIR promoter. GFP is between LIR and SIR and is driven from a 2X35S promoter. Rep is within the repliconDepositorInsertGFP
UsePlant t-dna plasmidTagsExpressionMutationPromoter2x35SAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
GAL4BD-VP16/pDON207
Plasmid#201230PurposeGAL4-VP16 transcription factorDepositorInsertGAL4BD-VP16
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGreen2x35SLUC
Plasmid#108228PurposePlant expression as translational fusions to FLUC with RLUC normalizationDepositorTypeEmpty backboneUseLuciferaseTagsFirefly LuciferaseExpressionPlantMutationPromoter2x35SAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA Scramble
Plasmid#162011PurposeLentiviral negative control vector containing scramble shRNADepositorInsertscramble shRNA
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETAipi
Plasmid#53277PurposeContains ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertidi
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAXY0002
Plasmid#179835Purposebinary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoterDepositorTypeEmpty backboneUseBinary vector for plant transformationTagsExpressionPlantMutationPromoterAvailable sinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-StayGold-9
Plasmid#197734PurposePhytobrick (MoClo) Level 0 PartDepositorInsertStayGold GFP
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b - PA
Plasmid#84863Purposebacterial expression of Bacillus anthracis protective antigenDepositorInsertPA
UseTagspelB signal sequenceExpressionBacterialMutationPromoterT7Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTA7001-DEST
Plasmid#71745PurposeGATEWAY compatible derivative of plant binary vector pTA7001 from Nam Chua's laboratory at The Rockefeller University, Dexamethasone inducibleDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterGAL4Available sinceFeb. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
SFG.CNb30_opt.IRES.eGFP
Plasmid#22493DepositorInsertCodon optimised Calcineurin B mutant 30 (PPP3R1 Human)
UseRetroviralTagsExpressionMutationL124T; K125-LA-InsPromoterAvailable sinceDec. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-IPT-9
Plasmid#197746PurposePhytobrick (MoClo) Level 0 PartDepositorInsertIsopentyl transferase (IPT)
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFBP_promoter_acceptor
Plasmid#189920PurposeBinary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZY101
Plasmid#73932PurposeThis binary vector, also named PTF101, is used as a standard plant transformation vector. For more information, please see http://plantsci.missouri.edu/muptcf/pzy101.htmlDepositorInsertbar
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
vHB8
Plasmid#193454PurposeExpression of GFP-Cas9-NLS and gRNA in A. castellaniiDepositorInsertGFP-Cas9-NLS
UseCRISPRTagsGFPExpressionMutationPromoterGAPDHAvailable sinceJan. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP2 MCR-1
Plasmid#118404PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.DepositorInsertMCR-1
UseTagsExpressionBacterialMutationPromoterpLacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC-GAMMAipi
Plasmid#53278PurposeHas ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coliDepositorInsertcrtYm
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLSL
Plasmid#51493PurposeT-DNA destination plasmid. Contains the bean yellow dwarf virus cis-acting elements (LIR and SIR) in an LIR-SIR-LIR orientation. Contains attR1 and attR2 sites between the LIR and SIR sequence.DepositorTypeEmpty backboneUsePlant t-dna plasmidTagsExpressionMutationPromotervirion-sense LIR and 2x35SAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ145-ZmUbi-tRNA
Plasmid#158403PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ135-tRNA2.0; assembly of 5 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134B2.0
Plasmid#167158PurposeGolden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-D156R (ttLbCas12a)
Plasmid#195366PurposeA temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12aDepositorInsertLbCas12a-D156R (ttLbCas12a)
UseCRISPR; Gateway compatible lbcas12a-d156r (ttlbca…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only