We narrowed to 1,843 results for: FAST
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Plasmid#194043PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
TagsHis10 and mEGFP,ExpressionInsectMutationY795APromoterPolyhedrinAvailable SinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RcSxph Y558A/I782A
Plasmid#194044PurposeProtein expression and characterizationDepositorInsertRana catesbeiana Saxiphilin
TagsHis10 and mEGFP,ExpressionInsectMutationY558A/I782APromoterPolyhedrinAvailable SinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
RNF169-Halo HRD
Plasmid#207083PurposeHomologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.DepositorInsertHaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINER3R
Plasmid#185883PurposeIn vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
ExpressionYeastMutationNoneAvailable SinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIT7A6
Plasmid#185896PurposeIn vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pINER4R
Plasmid#185884PurposeIn vivo gene amplification (HapAmp) of nerolidol synthetic module locus (25 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
ExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK Fus
Plasmid#186145PurposeFor expression of recombinant FAK using baculovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
TagsHis6ExpressionInsectMutationC-terminal linker swapped with FUS IDRPromoterpolyhedrin promoterAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lck
Plasmid#186130PurposeFor expression of recombinant Lck in SF9 cells.DepositorAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
FAK Pyk2
Plasmid#186146PurposeFor expresseion of recombinant FAK using baccolovirus in SF9 cellsDepositorInsertPTK2 (PTK2 Mouse)
TagsHis6ExpressionInsectMutationC-terminal linker swapped with Pyk2 linkerPromoterpolyhedrin promoterAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
Mdn1-deltaC
Plasmid#171603PurposeExpresses S. pombe Mdn1 a.a. 1-3911 in insect cells.DepositorInsertMdn1
TagsHis6 tagExpressionInsectMutationC-terminal deletion; a.a. 1-3911 onlyAvailable SinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSR657
Plasmid#65214PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-2 cap
UseAAVExpressionInsectPromoterp10 and polyhedrinAvailable SinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSR646
Plasmid#65215PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-6 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP
Plasmid#35511PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsEYFPExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSR660
Plasmid#65216PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-8 cap
UseAAVExpressionInsectPromoterP10 and polyhedrinAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor
Plasmid#86843PurposeHR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.DepositorInsertFLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
TagsFLAG-HaloTagExpressionMammalianPromoterEndogenous TERT promoterAvailable SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR651
Plasmid#65213PurposeBaculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cellsDepositorInsertsmodified AAV-2 rep78
modified AAV-1 cap
UseAAVExpressionInsectPromoterp10 and polyhedrinAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123A)-mCherry
Plasmid#35506PurposeAAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123APromoterCaMKIIaAvailable SinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
p130Cas Y15F
Plasmid#186154PurposeFor expressing recombinant p130Cas in insect cells.DepositorInsertBCAR1 (Bcar1 Rat)
TagsHis6 (Tev)ExpressionInsectMutation15Y residues mutated to AlaninePromoterpolyhedrinAvailable SinceJune 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(E17K)
Plasmid#86563PurposeExpresses human full-length Akt1(E17K) mutantDepositorAvailable SinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -