We narrowed to 3,583 results for: golden gate

1. pJet1.2 ermB cassette

   Plasmid

   #117121

   Purpose

   Erythromycin resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Erythromycin resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJJB1578

   Plasmid

   #218620

   Purpose

   Express Pex22, Pex6, Pex15, and Pex8

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   Pex22
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJet1.2 cat cassette

   Plasmid

   #117119

   Purpose

   Chloramphenicol resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Chloramphenicol resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJet1.2 kanR cassette

   Plasmid

   #117122

   Purpose

   Kanamycin resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Kanamycin resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBR_attB(bxb)_ccdB_lox_v2

   Plasmid

   #198040

   Purpose

   Bxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

   Type

   Empty backbone

   Use

   Cre/Lox and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR8/GW:U6

   Plasmid

   #85981

   Purpose

   Golden Gate Level 0 cassette encoding the U6 promoter

   Depositor

   Thomas Mock

   Article

   Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

   Insert

   T. pseudonana U6 promoter
   

   Use

   CRISPR; Diatom (t. pseudonana)

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Jan. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJPC210

   Plasmid

   #234532

   Purpose

   Int13 Recombinase switch; plant T-DNA

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

   Insert

   RUBY
   

   Use

   Tags

   Expression

   Plant

   Mutation

   none

   Promoter

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJPC063

   Plasmid

   #234533

   Purpose

   TRV2 expressing Int13

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

   Insert

   Integrase 13 recombinase
   

   Use

   Recombinase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJPC062

   Plasmid

   #234534

   Purpose

   TRV2 expressing CinH

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

   Insert

   CinH Recombinase
   

   Use

   Recombinase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJPC430

    Plasmid

    #234529

    Purpose

    CinH Recombinase switch; plant T-DNA

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Physiol. 2025 Mar 1;197(3):kiaf073

    Insert

    RUBY
    

    Use

    Tags

    Expression

    Plant

    Mutation

    none

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. G555

    Plasmid

    #216483

    Purpose

    Expression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.

    Depositor

    Christophe Danelon

    Insert

    plsB, plsC, cdsA, pssA
    

    Use

    Cell-free expression

    Tags

    Expression

    Mutation

    Promoter

    T7 for each gene

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGGZ003

    Plasmid

    #48869

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA left border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGZ001

    Plasmid

    #48868

    Purpose

    Empty destination vector for creating a GreenGate plant transformation plasmid with the plant resistance cassette at the T-DNA right border.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Type

    Empty backbone

    Use

    Golden gate compatible plant transformation vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBTK1050

    Plasmid

    #209540

    Purpose

    BTK Type 1 homology arm for Golden Gate assembly

    Depositor

    Jeffrey Barrick

    Article

    Lariviere et al bioRxiv 2023.09.19.558440

    Insert

    Upstream homology arm for recombination into the S. alvi wkB2 genome w/in the SALWKB2_RS11215/SALWKB2_RS11220 intergenic region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. GG-dest

    Plasmid

    #69538

    Purpose

    Destination plasmid for gRNA concatenation Golden Gate cloning

    Depositor

    Timo Otonkoski

    Article

    Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJet1.2 specR cassette

    Plasmid

    #117120

    Purpose

    Spectinomycin resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Spectinomycin resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EC10161

    Plasmid

    #154060

    Purpose

    Level 0.5 Golden Gate backbone, containing loxP sites.

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Type

    Empty backbone

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGGB003

    Plasmid

    #48821

    Purpose

    Provides a default dummy as GreenGate N-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    B-Dummy
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. BCJJ125

    Plasmid

    #117515

    Purpose

    Cas9_3 (with intron) Golden Gate Level 0

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BsaI:AATG:Cas9_3(intron):GCTT:BsaI
    

    Use

    CRISPR

    Tags

    2xFLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGGF005

    Plasmid

    #48846

    Purpose

    Provides a hygromycin resistance cassette as GreenGate module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    pUBQ10::HygromycinR::tOCS
    

    Use

    Golden gate compatible cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge