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TOPO-BC17

Plasmid

#84411

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC17

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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TOPO-BC18

Plasmid

#84412

Purpose

Encodes illumina barcodes for allele frequency sequencing (FREQ-Seq-Squared)

Depositor

J.J. Emerson

Article

Zhao et al G3 (Bethesda). 2023 Sep 30;13(10):jkad16

Insert

BC18

Use

Barcoding plasmids

Promoter

SP6/T7

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJMP2373

Plasmid

#160079

Purpose

Z. mobilis CRISPRi, promoter F, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2371

Plasmid

#160078

Purpose

Z. mobilis CRISPRi, promoter E, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2369

Plasmid

#160077

Purpose

Z. mobilis CRISPRi, promoter D, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2132

Plasmid

#160075

Purpose

Z. mobilis CRISPRi, promoter B, empty guide

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2093

Plasmid

#160074

Purpose

Z. mobilis CRISPRi, promoter B, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-HiGlpG- S116A, H196A

Plasmid

#158539

Purpose

Bacterial expression of rhomboid protease catalytic mutant HiGlpG-S116A,H196A

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

HiGlpG

Tags

GST tag

Expression

Bacterial

Mutation

S116A;H169A

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-delN-EcGlpG

Plasmid

#158537

Purpose

Bacterial expression of rhomboid protease EcGlpG without N terminus

Depositor

Sinisa Urban

Article

Mihaljevic et al Structure. 2020 Aug 10. pii: S0969-2126(

Insert

delta N-terminus EcGlpG

Tags

GST tag

Expression

Bacterial

Mutation

N-terminal deletion (1-86)

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMW-PahZ1 KT-1

Plasmid

#141134

Purpose

Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).

Depositor

Mitch Weiland

Article

Bolay et al Journal of Chemical Education

Insert

PahZ1

Tags

6x His

Expression

Bacterial

Mutation

Not the full-length gene. PahZ1 fragment starts a…

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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