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SFB-XPF-C236R

Plasmid

#247896

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged XPF C236R

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

XPF (ERCC4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

C236R

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SFB-XPF-P379S

Plasmid

#247897

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged XPF P379S

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

XPF (ERCC4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

P379S

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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SFB-XPF-R153P

Plasmid

#247893

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged XPF R153P

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Nucleic Acids Res. 2019 Nov 4;47(19):101

Insert

XPF (ERCC4 Human)

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Mutation

R153P

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX307-SFB-ZNF451

Plasmid

#247909

Purpose

Mammalian expression construct for N-terminal S protein-Flag-Streptavidin binding peptide (SFB)-tagged ZNF451

Depositor

Junjie Chen, Huimin Zhang

Article

Zhang et al Sci Adv. 2023 Dec 8;9(49):eadi6681. doi:

Insert

ZNF451 (ZNF451 Human)

Use

Lentiviral

Tags

S protein-Flag-Streptavidin binding peptide (SFB)

Expression

Mammalian

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CD59

Plasmid

#222307

Purpose

Synchronize the trafficking of CD59 from the ER.

Depositor

Franck Perez

Article

Fourriere et al J Cell Biol. 2019 Jul 1;218(7):2215-2231

Insert

Streptavidin-KDEL and CD59 fused to SBP-EGFP (CD59 Human)

Expression

Mammalian

Mutation

signal peptide removed

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBacHTA-NEDD4L_W1-L-W2-HECT

Plasmid

#187724

Purpose

Express His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 153-418+582-975 (NEDD4L Human)

Tags

His-TEV

Expression

Insect

Mutation

deletion of residues 1-152 and 419-581

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (381-975)_pcDNA3.1(+)

Plasmid

#187725

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-380

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L (492-975)_pcDNA3.1(+)

Plasmid

#187726

Purpose

Express his-tagged NEDD4L W3-W4-HECT in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 1-491

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)

Plasmid

#187727

Purpose

Express his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 226-382 removed (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 226-382

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)

Plasmid

#187728

Purpose

Express his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L with residues 418-495 removed (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Mammalian

Mutation

deletion of residues 418-495

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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