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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,800 results for: golden gate

Showing: 1701 - 1720 of 3800 results

  1. pSF522

    Plasmid
    #222588
    Purpose
    BB3 ZWF1 tet-on overexpression cassette with homologous flanks for integration at pyrG locus
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    ZWF1
    Use
    A. niger
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Promoter
    tet-on
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222588-plasmid-map-sequence-id-450454

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  2. pSF2

    Plasmid
    #222590
    Purpose
    gsdA disruption cassette via integration of pyrG from A. nidulans
    Depositor
    Matthias Steiger
    Article
    Matthias et al PLOS One
    Insert
    pyrG
    Use
    A. niger
    Expression
    Bacterial
    Mutation
    Internal BsaI and BpiI sites removed from insert
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    222590-plasmid-map-sequence-id-443810

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  3. pAIP4_4IPC

    Plasmid
    #234229
    Purpose
    Heterologous protein expression of replication protein A 70 kDa DNA-binding subunit in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4IPC (RPA1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234229-plasmid-map-sequence-id-464194

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  4. pAIP4_6F2Q

    Plasmid
    #234224
    Purpose
    Heterologous protein expression of galectin-3 (Gal-3) (CBP 35) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    6F2Q (LGALS3 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234224-plasmid-map-sequence-id-464189

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  5. pAIP4_2JHX

    Plasmid
    #234218
    Purpose
    Heterologous protein expression of rho GDP-dissociation inhibitor 1 (Rho GDI alpha) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2JHX (ARHGDIA Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234218-plasmid-map-sequence-id-464183

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  6. pAIP4_5HFD

    Plasmid
    #234189
    Purpose
    Heterologous protein expression of disks large homolog 4 (PSD-95) (SAP90) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5HFD (Dlg4 Rat)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234189-plasmid-map-sequence-id-464154

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  7. pAIP4_5MC7

    Plasmid
    #234155
    Purpose
    Heterologous protein expression of coatomer subunit zeta-1 (Zeta-1-coat protein) (Zeta-1 COP) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    5MC7 (COPZ1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234155-plasmid-map-sequence-id-464121

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  10. pRDB_092

    Plasmid
    #231110
    Purpose
    C>T base editor
    Depositor
    John Doench, David Root
    Insert
    TadA-CDd-nCas9-SpG-UGI
    Use
    CRISPR and Lentiviral
    Mutation
    D10A
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    231110-plasmid-map-sequence-id-459684

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  12. pVP293

    Plasmid
    #222062
    Purpose
    GreenGate CD bridge module containing filler sequence, backbone domesticated of Esp3I sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Insert
    Filler
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    Backbone Esp3i sites have been domesticated
    Available Since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222062-plasmid-map-sequence-id-437925

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  13. pVP294

    Plasmid
    #222063
    Purpose
    GreenGate DE bridge module containing filler sequence, backbone domesticated of Esp3I sites.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Insert
    Filler
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    Backbone Esp3i sites have been domesticated
    Available Since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222063-plasmid-map-sequence-id-447319

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  15. pVP076

    Plasmid
    #222028
    Purpose
    Empty spectinomycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Type
    Empty backbone
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    All Esp3i sites domesticated
    Available Since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222028-plasmid-map-sequence-id-447309

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  16. pVP077

    Plasmid
    #222029
    Purpose
    Empty Kanamycin resistant GreenGate destination vector based on pGGP-AG. Domesticated of Esp3I sites in the backbone and chloramphenicol resistance gene.
    Depositor
    Wayne Parrott
    Article
    Pennetti et al PLoS One. 2024 Sep 18;19(9):e0306008. do
    Type
    Empty backbone
    Use
    Synthetic Biology; Greengate compatible cloning v…
    Mutation
    All Esp3i sites domesticated
    Available Since
    Dec. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    222029-plasmid-map-sequence-id-447312

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Showing: 1701 - 1720 of 3800 results