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We narrowed to 5,049 results for: golden gate

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Showing: 1701 - 1720 of 5049 results

  1. MUT_S1-H1

    Plasmid
    #218394
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218394-plasmid-map-sequence-id-433909

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  2. PB_gRNA_S.pyogenes_scaffold_BB

    Plasmid
    #226429
    Purpose
    PiggyBac transposon vector backbone for cloning of sgRNA compatible with S.pyogenes dCas9 enzyme. BbsI Golden Gate site upstream of the scaffold for protospacer insertion. Puromycin selection marker.
    Depositor
    Alon Goren
    Type
    Empty backbone
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226429-plasmid-map-sequence-id-458099

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  3. pAIP4_2WWE

    Plasmid
    #234210
    Purpose
    Heterologous protein expression of PI3K-C2-gamma in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    2WWE (PIK3C2G Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234210-plasmid-map-sequence-id-464175

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  5. pTwist-Kan-HC-L0-TaBSS1-5PU

    Plasmid
    #242007
    Purpose
    L0 Pro+5U Golden Gate part of 5'UTR, core promoter, and upstream regulatory regions of Triticum aestivum MANY-NODED DWARF 1 (MND1) B-subgenome homoeolog (TraesCS7B02G413900)
    Depositor
    Cristobal Uauy
    Insert
    TaBSS1-5PU
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242007-plasmid-map-sequence-id-504528

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  6. pMB.MBP.JARID2

    Plasmid
    #247018
    Purpose
    Expression of MBP-JARID2 in insect cells, MBP tags are C3-cleavable (PreScission Protease)
    Depositor
    Chen Davidovich
    Insert
    JARID2 (JARID2 Human)
    Tags
    MBP
    Expression
    Insect
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247018-plasmid-map-sequence-id-493374

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  7. pMV_AA100

    Plasmid
    #245322
    Purpose
    Cas9 CRISPRko all-in-one positive control; targets CD55
    Depositor
    John Doench, David Root
    Insert
    Cas9, sgRNA CD55 (CD55 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245322-plasmid-map-sequence-id-488050

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  8. pMV_AA104

    Plasmid
    #245326
    Purpose
    Cas9 CRISPRa positive control guide; targets CD4
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD4 (CD4 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245326-plasmid-map-sequence-id-488034

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  9. pTwist-Kan-HC-L0-TaBSS1-3D

    Plasmid
    #242008
    Purpose
    L0 3U* Golden Gate part of 3' UTR and downstream regulatory regions of Triticum aestivum MANY-NODED DWARF 1 (MND1) B-subgenome homoeolog (TraesCS7B02G413900)
    Depositor
    Cristobal Uauy
    Insert
    TaBSS1-3D
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242008-plasmid-map-sequence-id-487882

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  10. pAttBFNeo

    Plasmid
    #244923
    Purpose
    attB Neo Resistant plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244923-plasmid-map-sequence-id-485479

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  11. pAttBFNeo2

    Plasmid
    #244924
    Purpose
    attB Neo Resistant plasmid for RMI targeting an attP site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244924-plasmid-map-sequence-id-485477

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  12. pAttPF3Neo

    Plasmid
    #244930
    Purpose
    attP Neo Resistant plasmid for RMI targeting an attB site
    Depositor
    Michael Nonet
    Article
    Nonet et al bioRxiv 2024.03.01.583017
    Type
    Empty backbone
    Use
    Phic31 recombinase mediated insertion (rmi)
    Expression
    Worm
    Promoter
    none
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    244930-plasmid-map-sequence-id-485475

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  14. pSG1016-2xBsaI-SaKKH_P2A_EGFP

    Plasmid
    #239461
    Purpose
    Codon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker.
    Depositor
    Alexis Komor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    239461-plasmid-map-sequence-id-476832

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  16. pAIP4_1T3Y

    Plasmid
    #234228
    Purpose
    Heterologous protein expression of coactosin-like protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1T3Y (COTL1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234228-plasmid-map-sequence-id-464193

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  17. pAIP4_3TOW

    Plasmid
    #234201
    Purpose
    Heterologous protein expression of multivesicular body subunit 12B (ESCRT-I complex subunit MVB12B) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3TOW (MVB12B Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234201-plasmid-map-sequence-id-464166

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  18. pAIP4_1KQX

    Plasmid
    #234181
    Purpose
    Heterologous protein expression of cellular retinol-binding protein type II in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1KQX (rbp2a Zebrafish)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234181-plasmid-map-sequence-id-464146

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  19. pAIP4_1AKO

    Plasmid
    #234162
    Purpose
    Heterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1AKO (xthA Escherichia coli K-12)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234162-plasmid-map-sequence-id-464128

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  20. pAIP4_4NFB

    Plasmid
    #234161
    Purpose
    Heterologous protein expression of paired immunoglobulin-like type 2 receptor alpha in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4NFB (PILRA Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234161-plasmid-map-sequence-id-464127

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Showing: 1701 - 1720 of 5049 results