We narrowed to 5,134 results for: golden gate

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pJET-TtrpC

Plasmid

#213467

Purpose

Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Terminator of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PgpdA

Plasmid

#213466

Purpose

Vector containing the gpdA promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of the glyceraldehyde-3-phosphate dehydrogenase

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PtrpC

Plasmid

#213465

Purpose

Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

Plasmid

#178986

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

Plasmid

#178990

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1397-C

Plasmid

#174563

Purpose

mitochondrial-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-C

Tags

FLAG

Expression

Plant

Promoter

PcUbi

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1397-N

Plasmid

#174562

Purpose

mitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-N

Tags

HA

Expression

Plant

Promoter

PcUbi

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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cp-DdCBE G1397-C

Plasmid

#174559

Purpose

Chloroplast-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-C

Tags

FLAG

Expression

Plant

Promoter

PcUbi

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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cp-DdCBE G1397-N

Plasmid

#174558

Purpose

Chloroplast-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Article

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-N

Tags

HA

Expression

Plant

Promoter

PcUbi

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pL0-BvCYP76AD1

Plasmid

#162529

Purpose

Beta vulgaris cytochrome P450 76AD1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

Depositor

Sam Brockington

Article

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

CYP76AD1

Use

Puc19-derived

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CB0026

Plasmid

#162285

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CB0246

Plasmid

#162286

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEPMY1CB0001

Plasmid

#162317

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0283

Plasmid

#162288

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0282

Plasmid

#162287

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0025

Plasmid

#162283

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pNB1-LOX

Plasmid

#154058

Purpose

Level 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosT

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

LoxP-flanked GUS and TtNAM-B1

Use

Synthetic Biology

Expression

Bacterial

Mutation

The TtNAM-B1 gene sequence was domesticated to re…

Promoter

ZmUbi

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0104

Plasmid

#117569

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtIPCS1 {AT3G54020} in A. thaliana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sa_gRNA_AtIPCS1 {AT3G54020} A. thaliana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0093

Plasmid

#117568

Purpose

Level1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting AtIPCS1 {AT3G54020} in A. thaliana

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Promoter_AtU6-26 + sgRNA_AtIPCS1 {AT3G54020} A. thaliana

Expression

Plant

Promoter

AtU6-26 (pICSL90002)

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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