We narrowed to 5,246 results for: golden gate

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pXTK074 [CDS_mRFP_PartType6]

Plasmid

#229311

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_mRFP_PartType6

Depositor

Michael Springer

Publication

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

mRFP

Expression

Bacterial

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK075 [CDS_sfGFP_PartType6]

Plasmid

#229312

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains CDS_sfGFP_PartType6

Depositor

Michael Springer

Publication

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

sfGFP

Expression

Bacterial

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK014 [Marker_SacB_PartType4]

Plasmid

#229251

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Marker_SacB_PartType4

Depositor

Michael Springer

Publication

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

SacB

Expression

Bacterial

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK019 [Origin_pBBR1_PartType1]

Plasmid

#229256

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_pBBR1_PartType1

Depositor

Michael Springer

Publication

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

pBBR1 Origin

Expression

Bacterial

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK020 [Origin_p15A_PartType1]

Plasmid

#229257

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_p15A_PartType1

Depositor

Michael Springer

Publication

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

p15A Origin

Expression

Bacterial

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pXTK023 [Origin_pSC101hs_PartType1]

Plasmid

#229260

Purpose

XanthoMoClo Golden Gate Part Plasmid for cloning, contains Origin_pSC101hs_PartType1

Depositor

Michael Springer

Publication

Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

Insert

pSC101hs Origin

Expression

Bacterial

Available since

March 14, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Publication

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA19_sgRNA-C0_M13ps

Plasmid

#231410

Purpose

M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

Depositor

Alfonso Jaramillo

Publication

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-C0

Use

Synthetic Biology

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pJET-TtrpC

Plasmid

#213467

Purpose

Vector containing the TrpC terminator for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Publication

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Terminator of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PgpdA

Plasmid

#213466

Purpose

Vector containing the gpdA promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Publication

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of the glyceraldehyde-3-phosphate dehydrogenase

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-PtrpC

Plasmid

#213465

Purpose

Vector containing the TrpC promoter for Golden Gate cloning in the Fg vector

Depositor

Kim Hammond-Kosack

Publication

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Promoter of tryptophan synthase of Aspergillus nidulans

Expression

Bacterial

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Publication

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

Plasmid

#178986

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

Plasmid

#178990

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1397-C

Plasmid

#174563

Purpose

mitochondrial-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-C

Tags

FLAG

Expression

Plant

Promoter

PcUbi

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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mt-DdCBE G1397-N

Plasmid

#174562

Purpose

mitochondrial-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-N

Tags

HA

Expression

Plant

Promoter

PcUbi

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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cp-DdCBE G1397-C

Plasmid

#174559

Purpose

Chloroplast-targeting DdCBE (split DddA, G1397-C) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-C

Tags

FLAG

Expression

Plant

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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cp-DdCBE G1397-N

Plasmid

#174558

Purpose

Chloroplast-targeting DdCBE (split DddA, G1397-N) Golden Gate destination vector

Depositor

Jin-Soo Kim

Publication

Kang et al Nat Plants. 2021 Jul;7(7):899-905. doi:

Insert

DdCBE G1397-N

Tags

HA

Expression

Plant

Promoter

PcUbi

Available since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pL0-BvCYP76AD1

Plasmid

#162529

Purpose

Beta vulgaris cytochrome P450 76AD1 coding sequence in pICH41308 MoClo Golden Gate level 0 acceptor for CDS1 modules.

Depositor

Sam Brockington

Publication

Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

Insert

CYP76AD1

Use

Puc19-derived

Expression

Bacterial

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CB0026

Plasmid

#162285

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Publication

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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