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p8552 pLIX-PTPN14 ΔPPXY3/4

Plasmid

#221645

Purpose

Doxycycline-inducible overexpression of human PTPN14 ΔPPXY3/4 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY3/4 (PTPN14 Human)

Use

Lentiviral

Tags

HA

Mutation

deletion of the third and fourth PPXY motifs as d…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8215 pHAGE N-V5 PTPN14 ΔPPXY1/2

Plasmid

#221646

Purpose

Constitutive ectopic expression of human PTPN14 ΔPPXY1/2 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY1/2 (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

deletion of the first and second (tandem) PPXY mo…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8180 pHAGE N-V5 PTPN14 ΔPPXY3/4

Plasmid

#221647

Purpose

Constitutive ectopic expression of human PTPN14 ΔPPXY3/4 mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 ΔPPXY3/4 (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

deletion of the third and fourth PPXY motifs as d…

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8189 pHAGE N-V5 PTPN14 C1121S

Plasmid

#221648

Purpose

Constitutive ectopic expression of human PTPN14 C1121S mutant

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

PTPN14 C1121S (PTPN14 Human)

Use

Lentiviral

Tags

V5

Mutation

C1121S mutation at the putative catalytic site

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8391 LentiCRISPRv2 Neo sgPTPN14-1

Plasmid

#221651

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-1 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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p8392 LentiCRISPRv2 Neo sgPTPN14-3

Plasmid

#221652

Purpose

Expression of sgRNA targeting the locus of human PTPN14

Depositor

Elizabeth White

Article

Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

Insert

sgPTPN14-3 (PTPN14 Human)

Use

CRISPR and Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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TLNRD1(FL)_pEGFP-C1

Plasmid

#224070

Purpose

Mamalian expression of full-length TLNRD1 fused to an N-terminal EGFP

Depositor

Ben Goult

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

tlnrd1 (TLNRD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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TLNRD1(4H)_pEGFP-N3

Plasmid

#224067

Purpose

Mamalian expression of the 4-helix bundle of TLNRD1 (residues 143-273) fused to a C-terminal EGFP

Depositor

Ben Goult

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

tlnrd1 (TLNRD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 26, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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hU6-DR_BsmBI_DR-EFS-RfxCas13d-NLS-2A-Puro-WPRE CasRx pre-gRNA backbone

Plasmid

#219823

Purpose

EF1α-driven expression of RfxCas13d in mammalian cells. For cloning of pre-guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. (Adapted from plasmid #138147)

Depositor

Philipp Maass

Insert

RfxCas13d

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a core promoter

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP-GFP-emerin

Plasmid

#222415

Purpose

To express emerin with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

Emerin (EMD Human)

Tags

FKBP-GFP

Expression

Mammalian

Available Since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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