We narrowed to 2,117 results for: D10

1. AAV-hSyn-SpdCas9-W3SL

   Plasmid

   #210708

   Purpose

   This Plasmid express hSyn promoter driven SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   hSyn

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP

   Plasmid

   #127560

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Cas9-T2A-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   EGFP-D102G see depositor comments below

   Promoter

   Available since

   July 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-CamKIIa-monSTIM1

   Plasmid

   #161796

   Purpose

   Lentiviral vector expressing monSTIM1 in neurons.

   Depositor

   Won Do Heo

   Article

   Kim et al Nat Commun. 2020 Jan 10;11(1):210. doi:

   Insert

   EGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   CamKIIa

   Available since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBabe_puro_DEST_Flag_FNDC3B

   Plasmid

   #45269

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   FNDC3B (FNDC3B Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pBJG1 T7-8xHis-OGT 5N5A

   Plasmid

   #154280

   Purpose

   Bacterial expression of full length human OGT with 5N5A TPR mutations (inefficient substrate binding). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

   Depositor

   Suzanne Walker

   Article

   Levine et al J Am Chem Soc. 2018 Mar 14;140(10):3510-

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   8x His, HRV3C protease site, and T7 leader

   Expression

   Bacterial

   Mutation

   N322A, N356A, N390A, N424A, N458A mutations; bact…

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-BE3RA-P2A-GFP-PGK-Puro

   Plasmid

   #110868

   Purpose

   Lentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-mAID-BE3

   Plasmid

   #113420

   Purpose

   Expresses mAID-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   mAID-BE3 (Aicda Mouse, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLY152

   Plasmid

   #130950

   Purpose

   dcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)

   Depositor

   Baojun Wang

   Article

   Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

   Inserts

   dcas9
   tetR
   sfgfp
   

   Use

   Synthetic Biology

   Tags

   ASV tag

   Expression

   Bacterial

   Mutation

   Mutations D10A and H840A on WT cas9. Synonymous m…

   Promoter

   PpspA-2G6 and Ptet

   Available since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. T7pr-His6-MBP-TEV-dCas9-BirA*

   Plasmid

   #159994

   Purpose

   Bacterial expression of dCas9-BirA*

   Depositor

   Jacob Corn

   Article

   Wang et al Mol Cell. 2020 Jul 16;79(2):221-233.e5.

   Insert

   dCas9-BirA*
   

   Use

   Tags

   6X-His, MBP, 3X-FLAG, NLS

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   T7

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-dSpCas9-HF1-VP64-6xHis

    Plasmid

    #92118

    Purpose

    Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-hA3A-eBE-Y132D

    Plasmid

    #113429

    Purpose

    Expresses hA3A-eBE-Y132D in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-eBE-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_Y132D

    Promoter

    CMV

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUHD-HA-AML1-ETO

    Plasmid

    #12430

    Depositor

    Dong-Er Zhang

    Article

    Rhoades et al Blood. 2000 Sep 15. 96(6):2108-15.

    Insert

    AML1-ETO (RUNX1, RUNX1T1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    BstXI-SacII fragment in pUHD-AML1-ETO replaced wi…

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pCMV_UdgX-EE-UdgX-NG-nCas9-RBMX

    Plasmid

    #163571

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9-RBMX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Promoter

    Available since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRK5 beta4 1355T

    Plasmid

    #16038

    Depositor

    Filippo Giancotti

    Article

    Nikolopoulos et al Mol Cell Biol. 2005 Jul . 25(14):6090-10

    Insert

    beta4 (ITGB4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1355T: amino acids 1-1355

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-hA1-BE3

    Plasmid

    #113418

    Purpose

    Expresses hA1-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA1-BE3 (APOBEC1 Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCMV_RBMX-eA3A-UdgX-NG-nCas9

    Plasmid

    #163551

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

    Promoter

    Available since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-BE3-P2A-GFP-PGK-Puro

    Plasmid

    #110867

    Purpose

    Lentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GGH-bio-His

    Plasmid

    #52066

    Purpose

    Expresses full-length Gamma-glutamyl hydrolase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    GGH (GGH Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV_UdgX-EE-UdgX-NG-nCas9

    Plasmid

    #163570

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    UdgX-EE-UdgX-NG-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

    Promoter

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hu-TLR4 299 snp-flag pDEST40

    Plasmid

    #42647

    Purpose

    destination vector for expression of human TLR4 299 SNP in mammalian cells

    Depositor

    Scott Friedman

    Article

    Guo et al Hepatology. 2009 Mar;49(3):960-8. doi: 1

    Insert

    TLR4 299 SNP (TLR4 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    D299G

    Promoter

    CMV

    Available since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only