We narrowed to 2,231 results for: D10

1. pJT173_GalL_A3A-R128A-BE3

   Plasmid

   #145122

   Purpose

   Expressing base editor A3A-R128A-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(R128A)-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   A3A(R128A); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT122_GalL_cCDA1-VQRBE3

   Plasmid

   #145087

   Purpose

   Expressing base editor cCDA1-VQRBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-VQRBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A, D1135V, R1335Q, T1337R)

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT113_GalL_cCDA1-xBE3

   Plasmid

   #145079

   Purpose

   Expressing base editor cCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-xBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. miniABEMax V82G nSaCas9

   Plasmid

   #135365

   Purpose

   Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   miniABEMax V82G nSaCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG RpH-LAMP1-3xFLAG

   Plasmid

   #163018

   Purpose

   expresses ratiometric sensor of lysosomal lumenal pH

   Depositor

   Massimiliano Stagi

   Article

   Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

   Insert

   RpH-LAMP1-3xFLAG (Lamp1 Mouse)
   

   Tags

   3XFLAG

   Expression

   Mammalian

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Str-KDEL-SBP-EGFP-LAMP1

   Plasmid

   #222317

   Purpose

   Synchronize the trafficking of LAMP1 from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LAMP1 fused to SBP-EGFP (LAMP1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Str-KDEL_SBP-EGFP-LAMP2

   Plasmid

   #222321

   Purpose

   Synchronize the trafficking of LAMP2 from the ER.

   Depositor

   Franck Perez

   Article

   Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

   Insert

   Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. dCas9-KRAB-MeCP2

   Plasmid

   #110821

   Purpose

   Improved dCas9 repressor-dCas9-KRAB-MeCP2

   Depositor

   George Church, Alejandro Chavez

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available Since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. Inducible Caspex expression

   Plasmid

   #97421

   Purpose

   For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics

   Depositor

   Samuel Myers, Steven Carr

   Article

   Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4

   Inserts

   Caspex
   T2A-Gfp
   

   Use

   CRISPR

   Tags

   3xFLAG and V5

   Expression

   Mammalian

   Mutation

   dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-Lyso-TORCAR

    Plasmid

    #64929

    Purpose

    Lysosome-targeted FRET biosensor for monitoring mTORC1 kinase activity; targeted to cytosolic face of lysosomal membrane.

    Depositor

    Jin Zhang

    Article

    Zhou et al Cell Rep. 2015 Mar 11. pii: S2211-1247(1

    Insert

    LAMP1-TORCAR (LAMP1 Human, Synthetic)
    

    Tags

    Cerulean, Full-length LAMP1, and YPet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry

    Plasmid

    #122132

    Purpose

    Express a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoter

    Depositor

    Stanley Qi

    Article

    Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

    Insert

    dCas9-10X GCN4-P2A-mCherry
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    dCas9 D10A & H840A

    Promoter

    TRE3G

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. ACBE-Q

    Plasmid

    #204608

    Purpose

    Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

    Available Since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Cbh_v5 AAV-CBE N-terminal

    Plasmid

    #137175

    Purpose

    AAV genome: expresses the N-terminal of v5 AAV-CBE from the Cbh promoter, and U6-sgRNA

    Depositor

    David Liu

    Article

    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

    Insert

    v5 AAV-CBE N-terminal; U6-protospacer
    

    Use

    AAV

    Mutation

    Cas9 D10A

    Promoter

    Cbh

    Available Since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-SpRYc-ABE8e-EGFP

    Plasmid

    #208340

    Purpose

    Expresses SpRYc-ABE8e in mammalian cells

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    ecTadA(8e)-nSpRYc
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CMV

    Available Since

    Aug. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. dCas9-KRAB

    Plasmid

    #110820

    Purpose

    Conventional dCas9 repressor-dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-hHDAC3

    Plasmid

    #98591

    Purpose

    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

    Depositor

    Zhaolan Zhou

    Article

    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

    Insert

    dCAS9-HDAC3 (HDAC3 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1A

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-hSyn-SpdCas9-W3SL

    Plasmid

    #210708

    Purpose

    This Plasmid express hSyn promoter driven SpdCas9

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    S. Pyogenes dCas9
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    hSyn

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-CAGGS-dCas9-KRAB

    Plasmid

    #110822

    Purpose

    PiggyBac compatible plasmid expressing dCas9-KRAB

    Depositor

    George Church, Alejandro Chavez

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CAGGS

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGFP-mTet1s pc3901

    Plasmid

    #201700

    Purpose

    Expresses of GFP-tagged mTet1s in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s (Tet1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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