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pcDNA3.1-HaloAKAR2.0-T2A-EGFP

Plasmid

#216488

Purpose

Expression of chemigenetic PKA biosensor (high basal brightness, medium dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.0

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-MmBorealin-OLLAS

Plasmid

#237439

Purpose

Expresses mouse Borealin tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

Depositor

Michael Lampson

Article

Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

Insert

Borealin (Cdca8 Mouse)

Tags

OLLAS

Expression

Mammalian

Promoter

T7

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGCS-3xHA-TMEM230

Plasmid

#225512

Purpose

TMEM230 expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Tags

3xHA

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGCS-3xHA-TMEM230triplemut

Plasmid

#225513

Purpose

TMEM230triplemut expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

TMEM230 (TMEM230 Human)

Tags

3xHA

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pGCS-3xFlag-ATP11B

Plasmid

#225511

Purpose

ATP11B expression vector

Depositor

Wade Harper

Article

Gonzalez-Lozano et al Nature. 2025 Jul;643(8070):252-261. doi:

Insert

ATP11B (ATP11B Human)

Tags

3xFlag

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Dot1LCD-CatMut

Plasmid

#235591

Purpose

Dox-inducible expression of control catalytic-mutant Dot1l CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Dot1l (Dot1l Mouse)

Use

CRISPR

Mutation

GS163-164RC

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Myc-rDMRdeltaTEAD

Plasmid

#235695

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Myc lacking Tead-binding motif with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Myc (Myc Mouse)

Use

Luciferase

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE Flag HA ITPA

Plasmid

#237998

Purpose

Inducible lentiviral expression of ITPA

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

ITPA (ITPA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE Flag HA MPG Catalytic Inactive

Plasmid

#237807

Purpose

Lentiviral vector expressing Catalytic Inactive MPG

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

MPG (MPG Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Cyr61-rDMR

Plasmid

#235692

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Cyr61 with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Cyr61 (Ccn1 Mouse)

Use

Luciferase

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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