We narrowed to 6,181 results for: nls

1. pmyc-GFP-TNRC6A-(925-934)-NLS-mut

   Plasmid

   #42020

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   contain aa925-934, R930A, R931A, R932A, R934A

   Promoter

   CMV

   Available Since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEJS2782 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

   Plasmid

   #240518

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion with restriction enzyme sites in the 3' UTR for use as microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Tags

   FLAG and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS1355 CMV-AcrIIC3-FLAGNLS-mCherry CMV-BFP

   Plasmid

   #226114

   Purpose

   Mammalian expression of AcrIIC3-mCherry fusion without microRNA target sites in the 3' UTR as a control reporter of microRNA repression. BFP expressed separately as a transfection control.

   Depositor

   Erik Sontheimer

   Article

   Kraus et al RNA. 2025 May 28:rna.080566.125. doi: 10

   Insert

   AcrIIC3
   

   Tags

   FLAG and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTol2-Ubi-lexPR-nls-GFP-pA (JDW 1316)

   Plasmid

   #229811

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus (1st generation)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   LexA transactivator-LexA-Operon-nls-GFP
   

   Use

   Tol2 based expression vector

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME nls EGFP WPRE bGH pA (JDW 488)

   Plasmid

   #224540

   Purpose

   A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   nls-EGFP-WPRE-bGH-pA
   

   Use

   Gateway cloning

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

   Plasmid

   #217970

   Purpose

   Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

   Depositor

   Robert Piper

   Article

   Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

   Insert

   2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
   

   Expression

   Mammalian

   Available Since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4

   Plasmid

   #219711

   Purpose

   Level 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.

   Depositor

   Jennifer Nemhauser

   Insert

   GG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
   

   Use

   Synthetic Biology

   Available Since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211844

   Purpose

   Plant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   3xNLS-HA and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   UBIQUITIN10

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CAG-EGFPnls barcode-210-SV40 polyA

   Plasmid

   #190876

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFPnls-barcode210
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLIK mCh-V5-NES-EGFP-EGFR NLS zeo

    Plasmid

    #192337

    Purpose

    Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-EGFR NLS

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    mCh-V5-NES-EGFP-EGFR NLS (EGFR Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK mCh-V5-NES-EGFP-Erbb2 NLS zeo

    Plasmid

    #192339

    Purpose

    Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-Erbb2 NLS

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    mCh-V5-NES-EGFP-Erbb2 NLS (Erbb2 Rat)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSLIK mCh-V5-NES-EGFP-SV40 NLS zeo

    Plasmid

    #192340

    Purpose

    Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-SV40 NLS

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    mCh-V5-NES-EGFP-SV40 NLS
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-NLS-ccdb-DddA(G1333C)-UGI-Puro

    Plasmid

    #173232

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-NLS-ccdb-DddA(G1333N)-UGI-Puro

    Plasmid

    #173233

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-NLS-ccdb-DddA(G1397C)-UGI-Puro

    Plasmid

    #173234

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-NLS-ccdb-DddA(G1397N)-UGI-Puro

    Plasmid

    #173235

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBK96 pHomL-ConLS-GFPdropout-ConR1-HomR (AmpR)

    Plasmid

    #179080

    Purpose

    Parent vector for for creating new markerless yeast integration cassettes with different promoters/genes/tags/terminators

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

    Plasmid

    #136884

    Purpose

    Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    engrailed repressor domain (en Fly)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. TetO-FUW-FLAG-NLS-VP64-MCS_N-term

    Plasmid

    #136886

    Purpose

    Doxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    VP64 activator domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TetO-FUW-FLAG-NLS-KRAB-MCS_N-term

    Plasmid

    #136888

    Purpose

    Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

    Depositor

    Moritz Mall

    Article

    Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

    Insert

    KRAB repressor domain
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Aug. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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