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p-mCherry

Plasmid

#249067

Purpose

Expresses nucleus-localized mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.

Depositor

John Dueber, David Schaffer

Article

Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:

Insert

mCherry

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Tet-pLKO-puro-shSF3B1

Plasmid

#248800

Purpose

Knockdown of endogenous SF3B1 with shRNA targeting 3'UTR

Depositor

Maayan Salton

Article

Baker et al RNA. 2025 Dec 24:rna.080661.125. doi: 10

Insert

shRNA targeting SF3B1 3'UTR

Use

Lentiviral

Promoter

hPGK

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd1/2 gRNA

Plasmid

#246562

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd1/2 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd1/2 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE-Puro-BirA

Plasmid

#246557

Purpose

Retroviral expression vector for secreted E. coli BirA, C-terminal FLAG tag

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

BirA

Use

Retroviral

Tags

FLAG

Promoter

MMLV LTR

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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HP17 sfGFP::P8

Plasmid

#235448

Purpose

Helper plasmid with sfGFP gene inserted at gene VIII locus

Depositor

Yolanda Schaerli

Article

Kusumawardhani et al Nat Commun. 2025 Apr 15;16(1):3569. doi:

Insert

Coding part of the M13KO7 helper phage genome, except gene VIII

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-HphMX6

Plasmid

#240973

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; HphMX6 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET-hisTdT

Plasmid

#216674

Purpose

Expresses his-tagged N-terminus truncated TdT in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

terminal deoxynucleotidyl transferase

Tags

his-tag

Expression

Bacterial

Mutation

1-138 truncation

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-C.a.URA3

Plasmid

#240970

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; C.a.URA3 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8d∆60-TRP1

Plasmid

#240968

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; TRP1 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-Pho8∆60-K.I.LEU2

Plasmid

#240969

Purpose

To endogenously tag S. cerevisiae proteins at their C-terminus with Pho8∆60; K.l.LEU2 selection marker

Depositor

Fulvio Reggiori

Article

Valdbjorn Kanne et al Autophagy Rep. 2025 May 31;4(1):2511724.

Insert

PHO8∆60 (PHO8 Budding Yeast)

Expression

Bacterial

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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