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HA-VHL W117R-pBabe-puro

Plasmid

#19239

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tryptophan 117 to Arginin

Available Since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

Plasmid

#167898

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

Plasmid

#134863

Purpose

Expresses EGFP-tagged endoplasmic reticulum marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

RAMP4 (SERP1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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shHIF2α(#2) (HIF2α-#3-pSUPER-retro-GFP/Neo)

Plasmid

#22132

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

synthethic oligonucleotide

Use

RNAi and Retroviral

Expression

Mammalian

Mutation

synthethic oligonucleotide encoding shRNA targeti…

Available Since

Feb. 1, 2010

Availability

Academic Institutions and Nonprofits only

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mCherry-TIS11B CPM2

Plasmid

#121143

Purpose

Mammalian expression of mCherry tagged TIS11B CPM2 (charge pattern mutant 2)

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Insert

ZFP36L1 (TIS11B) charge pattern mutant 2 (VEGFA Human)

Expression

Mammalian

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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px330-UFM1 sgRNA2

Plasmid

#134634

Purpose

contains sgRNA targeting human UFM1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 sgRNA2 (UFM1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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px330-UFM1 sgRNA1

Plasmid

#134633

Purpose

contains sgRNA targeting human UFM1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 sgRNA1 (UFM1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_mcherry

Plasmid

#167886

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
pCNL-N1_vinculin

Plasmid

#65691

Purpose

Expresses Vinculin-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

vinculin (Vcl Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
mADAM17deltaMP in pQCXIP cFLAG

Plasmid

#19144

Depositor

Zena Werb

Article

Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

Insert

ADAM17 (Adam17 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

delta 189-378

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pCaRNA3-eIFiso4E120(+)

Plasmid

#86092

Purpose

This vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.

Depositor

Aiming Wang

Article

Cui et al Plant Biotechnol J. 2016 Aug 27. doi: 10

Insert

eIFiso4E

Expression

Plant

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

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hGFAP R239C

Plasmid

#169476

Purpose

Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

EGFP-hGFAP (GFAP Human)

Tags

EGFP

Expression

Mammalian

Mutation

Changed R 239 to C

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pETconNK-TEM1(S70A,D179G)

Plasmid

#81166

Purpose

pETconNK with TEM1(S70A,D179G) beta-lactamase

Depositor

Timothy Whitehead

Article

Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

Insert

TEM-1 beta-lactamase

Tags

c-myc

Expression

Bacterial and Yeast

Mutation

Deleted the sec transport tag. Plasmid encodes H2…

Promoter

GAL1 and gal1

Available Since

Feb. 21, 2017

Availability

Academic Institutions and Nonprofits only

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px330-UFL1 sgRNA2

Plasmid

#134636

Purpose

contains sgRNA targeting human UFL1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFL1 sgRNA2 (UFL1 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRZ-mNanog-YNL

Plasmid

#65717

Purpose

mNanog reporter plasmid (expresses YNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

murine Nanog promoter and YNL

Use

Lentiviral and Luciferase

Available Since

July 22, 2015

Availability

Academic Institutions and Nonprofits only

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px330-UFL1 sgRNA1

Plasmid

#134635

Purpose

contains sgRNA targeting human UFL1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFL1 sgRNA1 (UFL1 Human)

Expression

Mammalian

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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XE241 Rat frizzled-1-GFP-CS2P+

Plasmid

#16817

Depositor

Randall Moon

Article

Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

Insert

frizzled-1 (Fzd1 Rat)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pDNA3-Cerulean-FHA1-NES

Plasmid

#138202

Purpose

Encodes N-terminal (PAABD) fragment of FRET-based bimolecular kinase activity reporters (BimKARs); cytosol targeted.

Depositor

Jin Zhang

Article

Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

Insert

Cerulean-FHA1-NES

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-mCherry_SNAP_SNAPV_PACT_Dead

Plasmid

#173073

Purpose

Dead SNAP tag at the centrosome

Depositor

Heidi Hehnly

Article

Bucko et al Elife. 2019 Dec 24;8. pii: 52220. doi: 1

Insert

mCherry-SNAP-SNAP-Varied-PACT

Tags

mCherry-SNAP-SNAP-Varied

Expression

Mammalian

Mutation

AA144 in both SNAP tags changed from a cysteine t…

Promoter

CMV IE94

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEYFP-Sec16A

Plasmid

#36156

Purpose

Expresses Sec16A as a YFP fusion

Depositor

David Stephens

Article

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Insert

Sec16A (SEC16A Human)

Tags

EYFP

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only