We narrowed to 5,293 results for: pcDNA3.1+

1. pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

   Plasmid

   #171435

   Purpose

   Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   Lifeact-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-KozATG-dL5-2XG4S-mCer3

   Plasmid

   #73207

   Purpose

   Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian cells, penetrates nucleus. (MBIC5, dL5**, FAP)

   Depositor

   Marcel Bruchez

   Article

   Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

   Insert

   KozATG-dL5-2XG4S-mCer3
   

   Use

   Tags

   The FAP and mCerulean3 are fused with 2 copies of…

   Expression

   Mammalian

   Mutation

   The dL5** FAP is E50D, L89S, also known as E52D/L…

   Promoter

   CMV

   Available since

   March 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. (133) pcDNA3.1-Nup62-Flag-EPEA

   Plasmid

   #160744

   Purpose

   Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   Nup62-Flag-EPEA
   

   Use

   Tags

   EPEA and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 FLAG-SIV-MAC251-vpx

   Plasmid

   #115842

   Purpose

   Encodes codon optimized FLAG tagged SIVMAC251-Vpx

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   SIVMAC251-Vpx
   

   Use

   Tags

   3x-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1-HsPcdh21-Myc-Flag-His

   Plasmid

   #210818

   Purpose

   Mammalian overexpression vector for C-terminally Myc and Flag and His tagged human Pchd21 (WT)

   Depositor

   Luke Chao

   Article

   Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

   Insert

   Protocadherin 21 (CDHR1 Human)
   

   Use

   Tags

   Myc, 3x Flag, 10x His

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5

   Plasmid

   #225244

   Purpose

   Mammalian expression of iPASnFR-L fused to Rab5

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-Flag-H2A K118-119R

   Plasmid

   #63564

   Purpose

   Expression of human H2A with K118R and K119R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 118 and Lysine 119 are now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1V5-His Snk/Plk2 T239A

   Plasmid

   #22219

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   single amino acid substitution mutant, threonine …

   Promoter

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

9. Human NCKX2-AS-long-FlagTag (pcDNA3.1+)

   Plasmid

   #75203

   Purpose

   Mammalian expression of SLC24A2

   Depositor

   Jonathan Lytton

   Article

   Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

   Insert

   SLC24A2 (SLC24A2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   alternatively spliced exon present

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3.1-Flag-H2A K13-15R

    Plasmid

    #63563

    Purpose

    Expression of human H2A with K13R and K15R tagged with Flag

    Depositor

    Titia Sixma

    Article

    Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

    Insert

    H2A (H2AC20 Human)
    

    Use

    Tags

    Flag (MDYKDDDDKVPRIQ)

    Expression

    Mammalian

    Mutation

    Lysine 13 and Lysine 15 are now Arginine

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

    Plasmid

    #171428

    Purpose

    Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    FKBP-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

    Plasmid

    #69906

    Purpose

    Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ciRS7 (CDR1-AS Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-2xFLAG-2xSTREP (empty vector)

    Plasmid

    #172604

    Purpose

    Empty vector for the cloning of an N-terminal 2xFLAG-2xSTREP-tagged gene of interest, and expression in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Use

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid

    #112157

    Purpose

    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

    Depositor

    Ben Giepmans

    Article

    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

    Insert

    anti-GFP FLIPPER-body
    

    Use

    Tags

    His and Thrombin cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 CARD8-TEV-GFP-FLAG

    Plasmid

    #169991

    Purpose

    Mammalian purification construct for CARD8

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    TEV-GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 T21R-vhhGFP4-T2A-mCherry

    Plasmid

    #225587

    Purpose

    Mammalian expression vector for TRIM21 RING-vhhGFP4 anti-GFP degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

    Depositor

    William McEwan

    Article

    Benn et al Science. 2024 Aug 30;385(6712):1009-1016

    Insert

    T21R-vhhGFP4-T2A-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-KozATG-dL5-2XG4S-actin

    Plasmid

    #73262

    Purpose

    Expresses dL5(E52D)-actin fusion within mammalian cells. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Yan et al Chemphyschem. 2014 Mar 17;15(4):687-95.

    Insert

    KozATG-dL5-2XG4S-actin (ACTB Human, Synthetic)
    

    Use

    Tags

    The FAP and actin are fused with 2 copies of a G4…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

    Plasmid

    #86885

    Purpose

    C-terminal part of the split-BioID

    Depositor

    Mathieu Bollen

    Article

    Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

    Insert

    BirA* (AA141-321)- HA- NIPP1]
    

    Use

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    BirA Amino Acids 141-321

    Promoter

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 2xFLAG-2xSTREP RAD51 (F129A)

    Plasmid

    #158952

    Purpose

    Express human RAD51(F129A) mutant in mammalian cells

    Depositor

    Michele Pagano

    Article

    Marzio et al Mol Cell. 2019 Jan 17;73(2):224-237.e6.

    Insert

    RAD51 (RAD51 Human)
    

    Use

    Tags

    2X FLAG 2X STREP

    Expression

    Mammalian

    Mutation

    changed phenylalanine 129 to alanine

    Promoter

    CMV

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1 CMV mCherry-eGFP BGH

    Plasmid

    #92280

    Purpose

    Control FRET construct; tandem mCherry-eGFP fusion

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    mCherry-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge